In vivo role of novel innate immune factors during viral infection

• Project/Area Number: 22790450
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Hokkaido University
• Principal Investigator: OSHIUMI Hiroyuki 北海道大学, 大学院・医学研究科, 講師 (50379103)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 自然免疫 / ウイルス / インターフェロン / 核酸 / ユビキチン / インフルエンザ / C型肝炎 / シグナル / RIG-I / I型インターフェロン / Riplet / 免疫 / 炎症

Research Abstract

Innate immune response is essential for the protection against viral infection, such as novel flu or Hepatitis C virus. We have previously isolated a novel protein, Riplet. We constructed Riplet deficient mice and examined its phenotype. We found that Riplet is essential for type I interferon production, which possesses strong antiviral activity, in response to viral infection in vivo.

Research Products

• [Journal Article] Ubiquitin-mediated modulation of the cytoplasmic viral RNA sensor RIG-I (2012)
  • Author(s): Oshiumi, H., M.Matsumoto, T.Seya
  • Journal Title: J.Biochem. Volume: 151 Issue: 1 Pages: 5-11
  • DOI: 10.1093/jb/mvr111
  • NAID: 10031166037
  • Peer Reviewed
• [Journal Article] The Toll-like receptor 3-mediayed antiviral response is important for protection against poliovirus infection in poliovirus receptor transgenic mice (2012)
  • Author(s): Abe, Y., K.Fujii, N.Nagata, O.Takeuchi, S.Akira, H.Oshiumi, M.Matsumoto, T.Seya, S.Koike
  • Journal Title: J.Virol Volume: 86 Issue: 1 Pages: 185-194
  • DOI: 10.1128/jvi.05245-11
  • Peer Reviewed
• [Journal Article] Toll-like receptor 3 signaling converts tumor-supporting myeloid cells to tumoricidal effectors (2012)
  • Author(s): H.Shime
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Issue: 6 Pages: 2066-2071
  • DOI: 10.1073/pnas.1113099109
  • Peer Reviewed
• [Journal Article] DDX60, a DHXD/H box helicase, is a novel antiviral factor promoting RIG-I-like receptor-mediated signaling (2011)
  • Author(s): Miyashita, M., H.Oshiumi, M.Matsumoto, T.Seya
  • Journal Title: Mol.Cell Biol Volume: 31 Issue: 18 Pages: 3802-3819
  • DOI: 10.1128/mcb.01368-10
  • Peer Reviewed
• [Journal Article] The TLR3-TICAM-1 pathway is mandatory for innate immune responses to poliovirus infection. (2011)
  • Author(s): Oshiumi, H., M. Okamoto, K. Fujii, T. Kawanishi, M. Matsumoto, S. Koike, and T. Seya.
  • Journal Title: J. Immunol. Volume: 187 Issue: 10 Pages: 5320-5327
  • DOI: 10.4049/jimmunol.1101503
  • Peer Reviewed
• [Journal Article] Development of mouse hepatocyte lines permissive for hepatitis C virus (HCV) (2011)
  • Author(s): Aly, H.H., H.Oshiumi, H.Shime, M.Matsumoto, T.Wakita, K.Shimotohno, T.Seya
  • Journal Title: Plos ONE Volume: 6 Issue: 6 Pages: e21284-e21284
  • DOI: 10.1371/journal.pone.0021284
  • NAID: 120003169041
  • Peer Reviewed
• [Journal Article] The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection (2010)
  • Author(s): Oshiumi H, Miyashita M, Inoue N, Okabe M, Matsumoto M, Seya T
  • Journal Title: Cell Host Microbe Volume: 8 Issue: 6 Pages: 496-509
  • DOI: 10.1016/j.chom.2010.11.008
  • NAID: 120002725554
  • Peer Reviewed
• [Journal Article] The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune response to RNA virus infection (2010)
  • Author(s): 押海裕之
  • Journal Title: Cell Host & Microbe Volume: 8 Pages: 496-509
  • NAID: 120002725554
  • Peer Reviewed
• [Journal Article] Hepatitis C virus core protein abrogates the DDX3 function that enhances IPS-1-mediated IFN-beta induction (2010)
  • Author(s): 押海裕之
  • Journal Title: Plos One Volume: 5
  • NAID: 120002697703
  • Peer Reviewed
• [Journal Article] DEAD BOX3(DDX3) helicases binds the RIG-I adaptor IPS-1 to up-regulate IFN-beta inducing potential (2010)
  • Author(s): 押海裕之
  • Journal Title: European Journal of Immunology Volume: 40 Pages: 940-948
  • Peer Reviewed
• [Presentation] ウイルスによる自然免疫活性化とその抑制機構 (2012)
  • Author(s): 押海裕之
  • Organizer: 日本薬学会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-31
• [Presentation] ウイルスによる自然免疫活性化とその抑制機構 (2012)
  • Author(s): 押海裕之
  • Organizer: 日本薬学会
  • Place of Presentation: 北海道大学(札幌市)(招待講演)
  • Year and Date: 2012-03-31
• [Presentation] The ubiquitin ligase Riplet is essential for RIG-I dependent type I interferon production in response to RNA virus infection (2011)
  • Author(s): 押海裕之
  • Organizer: 日本分子生物学会
  • Place of Presentation: バシフィコ横浜(神奈川)
  • Year and Date: 2011-12-14
• [Presentation] The ubjquitin ligase Riplet is essential for RIG-I dependent type I interferon production in response to RNA virus infection (2011)
  • Author(s): 押海裕之
  • Organizer: 日本分子生物学会
  • Place of Presentation: バシフィコ横浜(神奈川)
  • Year and Date: 2011-12-14
• [Presentation] Regulatory mechanism of RIG-I by Riplet ubiquitin ligase (2011)
  • Author(s): 押海裕之
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-27
• [Presentation] Regulatory mechanism of RIG-I by Riplet ubiquitin ligase (2011)
  • Author(s): 押海裕之
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ(千葉県)(招待講演)
  • Year and Date: 2011-11-27
• [Presentation] Riplet ubiquitin ligase is essential for RIG-I dependent type I interferon production (2011)
  • Author(s): 押海裕之
  • Organizer: Keystone Symposium
  • Place of Presentation: Santa Fe Community Convention center (アメリカ合衆国)
  • Year and Date: 2011-02-15
• [Presentation] TICAM-1/TRIF, a TLR3 adaptor, is essential for protection against poliovirus infection. (2010)
  • Author(s): 押海裕之
  • Organizer: 国際免疫学会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-08-23
• [Presentation] 細胞質内ウイルスRNA認識センサーRIG-Iをユビキチン化するRiplet分子のノックアウトマウス作成と機能解析 (2010)
  • Author(s): 押海裕之
  • Organizer: インターフェロンサイトカイン学会
  • Place of Presentation: 北九州国際会議場(福岡県)
  • Year and Date: 2010-06-25
• [Remarks]
  • URL: http://www.hucc.hokudai.ac.jp/~e20536/