In vivo analysis of molecular mechanism for pre-TCR-mediatedβ-selection

• Project/Area Number: 22790470
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Kyushu University
• Principal Investigator: ISHIKAWA Eri 九州大学, 生体防御医学研究所, 助教 (20546478)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リンパ球 / T細胞分化 / プレT細胞受容体 / 自発的シグナル / 免疫学

Research Abstract

The ligand for pre-TCR has not been identified for many years. To verifya model that we have recently proposed in vivo, we established mutant pre-TCRαchain(pTα) knock-in mice that lacks charged residues. From analysis of the mice, we first demonstrated that immature antigen receptor spontaneously induces signals through charged residues in pTαin vivo.

Research Products

• [Journal Article] Germ-line elimination of electric charge on pre-T-cell receptor(TCR) impairs autono mous signaling forβ-selection and TCR reper toire formation. (2010)
  • Author(s): Eri Ishikawa, Yasunobu Miyake, Hiromi tsu Hara, Takashi Saito and Sho Yamas aki
  • Journal Title: Proc. NatlAcad. Sci. USA Volume: 107 Pages: 19979-19984
  • Peer Reviewed
• [Journal Article] Germ-line elimination of electric charge on pre-T-cell receptor (TCR) impairs autonomous signaling for beta-selection and TCR repertoire formation. (2010)
  • Author(s): 石川絵里
  • Journal Title: Proc.Natl.Acad.Sci.USA. Volume: 107(46) Pages: 19979-19984
  • Peer Reviewed
• [Presentation] レパトア形成におけるβ-selectionの意義/The essential role ofβ-selection in TCR repertoire formation (2011)
  • Author(s): ISHIKAWA Eri, MIYAKE Yasunobu, SAITO Takashi and Yamasaki Sho TCR
  • Organizer: 第40回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2011-11-27
• [Presentation] TCRレパトア形成におけるβ-selectionの意義 (2011)
  • Author(s): 石川絵里
  • Organizer: 第40回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2011-11-27
• [Presentation] TCRレパトア形成におけるβ-selectionの意義 (2010)
  • Author(s): 石川絵里、三宅靖延、斉藤隆、山崎晶
  • Organizer: 第20回Kyoto T Cell Conference
  • Place of Presentation: 京都大学医学部芝蘭会館
  • Year and Date: 2010-06-04
• [Presentation] TCRレパトア形成におけるβ-selectionの意義 (2010)
  • Author(s): 石川絵里
  • Organizer: 第20回 Kyoto T Cell Conference
  • Place of Presentation: 京都大学医学部芝蘭会館
  • Year and Date: 2010-06-04
• [Remarks] 研究室ホームページ
  • URL: http://www.bioreg.kyushu-u.ac.jp/labo/molimm/