Cell therapy based on high-efficiency delivery of mesenchymal stem cells into the injured brain
| Project/Area Number |
22790508
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied pharmacology
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 骨髄間葉系幹細胞 / 脳血管内皮細胞 / 血液脳関門 / リアルタイムイメージング / 細胞治療 / in vitro BBBモデル / 脳細胞傷害 / MAPキナーゼ / 血管内皮細胞 / グリーンラット |
|---|
| Research Abstract |
The goal of this study is to achieve high-efficiency delivery of intravenously injected mesenchymal stem cells(MSCs) into the injured brain. We developed an in vitro culture system consisting of rat brain microvascular endothelial cells(BMECs) and bone marrow-derived MSCs. Live cell imaging using this system revealed that MSCs transmigrated across BMEC monolayers via a paracellular route. In addition, inhibition of MAP kinases affected transmigration of MSCs across BMEC monolayers.
|
Report
(3 results)
Research Products
(10 results)
