Cell therapy based on high-efficiency delivery of mesenchymal stem cells into the injured brain

• Project/Area Number: 22790508
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied pharmacology
• Research Institution: Hokkaido University
• Principal Investigator: KATAYAMA Takahiro 北海道大学, 大学院・薬学研究院, 助教 (90399957)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 骨髄間葉系幹細胞 / 脳血管内皮細胞 / 血液脳関門 / リアルタイムイメージング / 細胞治療 / in vitro BBBモデル / 脳細胞傷害 / MAPキナーゼ / 血管内皮細胞 / グリーンラット

Research Abstract

The goal of this study is to achieve high-efficiency delivery of intravenously injected mesenchymal stem cells(MSCs) into the injured brain. We developed an in vitro culture system consisting of rat brain microvascular endothelial cells(BMECs) and bone marrow-derived MSCs. Live cell imaging using this system revealed that MSCs transmigrated across BMEC monolayers via a paracellular route. In addition, inhibition of MAP kinases affected transmigration of MSCs across BMEC monolayers.

Research Products

• [Journal Article] Mesenchymal stem cells transmigrate across brain microvascular endothelial cell monolayers through transiently formed inter-endothelial gaps (2011)
  • Author(s): Matsushita T, Kibayashi T, Katayama T, Yamashita Y, Suzuki S, Kawamata J, Honmou O, Minami M, Shimohama S
  • Journal Title: Neurosci Lett Volume: 502 Issue: 1 Pages: 41-45
  • DOI: 10.1016/j.neulet.2011.07.021
  • Peer Reviewed
• [Presentation] 骨髄間葉系幹細胞の血液脳関門通過機構における動態解析 (2012)
  • Author(s): 片山貴博
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2012-03-14
• [Presentation] Mesenchymal stem cells transmigrate across brain microvascular endothelial cell monolayers through the transiently formed interendothelial gaps Neuroscience2011 (2011)
  • Author(s): 片山貴博
  • Organizer: Society of Neuroscience 41st annual meeting Walter E
  • Place of Presentation: Washington Convention Center(USA)
  • Year and Date: 2011-11-14
• [Presentation] Mesenchymal stem cells transmigrate across brain microvascular endothelial cell monolayers through the transiently formed interendothelial gaps (2011)
  • Author(s): Takahiro Katayama
  • Organizer: Neuroscience2011, Society of Neuroscience 41st annual meeting
  • Place of Presentation: Walter E.Washington Convention Center (USA)
  • Year and Date: 2011-11-14
• [Presentation] In vitro血液脳関門モデルを用いた骨髄間葉系幹細胞の動態解析 (2011)
  • Author(s): 木林達也
  • Organizer: 第62回日本薬理学会北部会
  • Place of Presentation: 江陽グランドホテル(宮城県)
  • Year and Date: 2011-09-29
• [Presentation] インビトロ血液脳関門モデルを用いた骨髄間葉系幹細胞の脳実質内浸潤機構の解析 (2011)
  • Author(s): 片山貴博
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 瑠璃光(石川県)
  • Year and Date: 2011-09-27
• [Presentation] 骨髄間葉系幹細胞の脳実質内浸潤機構の解析 (2011)
  • Author(s): 松下隆司
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-15
• [Presentation] In vitro血液脳関門モデルを用いた骨髄間葉系幹細胞の動態解析 (2011)
  • Author(s): 木林達也
  • Organizer: 日本薬学会北海道支部第136回例会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-05-21
• [Presentation] インビトロ血液脳関門モデルを用いた骨髄幹細胞イメージング (2010)
  • Author(s): 松下隆司
  • Organizer: 第51回日本神経学会総会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2010-05-20
• [Presentation] インビトロ血液脳関門モデルを用いた骨髄幹細胞イメージング (2010)
  • Author(s): 松下隆司
  • Organizer: 第51回日本神経学会総会
  • Place of Presentation: 東京(東京国際フォーラム)
  • Year and Date: 2010-05-20