X-ray crystallographic analysis of an ABC transporter

• Project/Area Number: 22790512
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied pharmacology
• Research Institution: Kyoto University
• Principal Investigator: KODAN Atsushi 京都大学, 物質.細胞統合システム拠点, 研究員 (80360543)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 生体分子 / 蛋白質 / 薬学 / 癌 / ABC蛋白質 / X線結晶構造解析 / 創薬

Research Abstract

Understanding the mechanism of action of ABC proteins and developing new therapeutic strategies that target ABC proteins would be considerably accelerated with the three-dimensional structures. Crystal structure of an exporter-type ABC transporter from a unicellular eukaryote was determined by the multiwavelength anomalous dispersion(MAD) method using the X-ray diffraction data measured at SPring-8. The resolution obtained in this study is best among those of eukaryotic ABC proteins ever reported to date.

Research Products

• [Journal Article] Deleting two C-terminalα-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP (2010)
  • Author(s): Kanako Terakado, Atsushi Kodan, Hiroaki Nakano, Yasuhisa Kimura, Kazumitsu Ueda, Toru Nakatsu, and Hiroaki Kato
  • Journal Title: Acta Crystallographica Section D. Biological Crystallography Volume: D66 Issue: 3 Pages: 319-323
  • DOI: 10.1107/s0907444909055504
  • Peer Reviewed
• [Journal Article] Deleting two C-terminal α-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP. (2010)
  • Author(s): Terakado, K., Kodan, A., Nakano, H., Kimura, Y., Ueda, K., Nakatsu, T., Kato, H.
  • Journal Title: Acta Crystallographica Section D-Biological Crystallography Volume: D66 Pages: 319-323
  • Peer Reviewed
• [Presentation] X-ray crystallographic analysis of an exporter-type ABC transporter (2012)
  • Author(s): Atsushi Kodan
  • Organizer: ABC2012, the 4th FEBS Special Meeting
  • Place of Presentation: オーストリア(インスブルク)
  • Year and Date: 2012-02-29
• [Presentation] X-ray crystallographic analysis of an exporter-type ABC transporter (2012)
  • Author(s): Atsushi Kodan, Tomohiro Yamaguchi, Toru Nakatsu, Keita Sakiyama, Akane Fujioka, Kazumitsu Ueda, and Hiroaki Kato
  • Organizer: the 4th FEBSSpecial Meeting"ATP-Binding Cassette Proteins : From Multidrug Resistance to Genetic Disease
  • Place of Presentation: Innsuburuck, Austria
• [Presentation] 好熱性紅藻由来の基質排出型ABCトランスポーターの基質特異性の解析/Substrate specificity analysis of an exporter type ABC transporter from athermophilic eukaryote (2011)
  • Author(s): 山口知宏、崎山慶太、佐藤友美、小段篤史、中津亨、植田和光、加藤博章
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: 吹田
  • Year and Date: 2011-06-09
• [Presentation] Characterization of multi-drugexporter activity of an ABC transporter from a thermophiliceukaryote (2011)
  • Author(s): 山口知宏、崎山慶太、地主遼平、佐藤友美、小段篤史、中津亨、植田和光、加藤博章
  • Organizer: Cyanidioschyzon merolae日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-30
• [Presentation] X-ray crystallographic analysis of an exporter-type ABC transporter (2011)
  • Author(s): Atsushi Kodan, Tomohiro Yamaguchi, Toru Nakatsu, Keita Sakiyama, Akane Fujioka, Kazumitsu Ueda, and Hiroaki Kato
  • Organizer: Joint International Symposium"ABC2011 in Kyoto-ABC Proteins/Membrane Meso-domains/ES-iPS cells"
  • Place of Presentation: Kyoto, Japan
• [Presentation] 好熱性紅藻由来ABCトランスポーターの多剤排出活性の特徴 (2010)
  • Author(s): 山口知宏、崎山慶太、池口圭司、地主遼平、佐藤友美、渡辺文太、小段篤史、中津亨、平竹潤、植田和光、加藤博章
  • Organizer: 第32回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2010-11-29
• [Presentation] 好熱性紅藻由来ABCトランスポーターの多剤排出活性の特徴 (2010)
  • Author(s): 山口知宏、崎山慶太、池口圭司、地主遼平、佐藤友美、渡辺文太、小段篤史、中津亨、平竹潤、植田和光、加藤博章
  • Organizer: 第32回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 富山、富山国際会議場
  • Year and Date: 2010-11-29
• [Presentation] 好熱性紅藻Cyanidioschyzonmerolae由来のABCトランスポーターの発現、精製、及び機能解析 (2010)
  • Author(s): 崎山慶太、山口知宏、小段篤史、中津亨、佐藤友美、植田和光、加藤博章
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-18
• [Presentation] 好熱性紅藻Cyanidioschyzon merolae由来のABCトランスポーターの発現、精製、及び機能解析 (2010)
  • Author(s): 崎山慶太、山口知宏、小段篤史、中津亨、佐藤友美、植田和光、加藤博章
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌、札幌コンベンションセンター
  • Year and Date: 2010-06-18
• [Presentation] Functional role of the linker region in the mechanism of human multidrug efflux transporter P-glycoprotein (2010)
  • Author(s): Tomomi Sato, Atsushi Kodan, YasuhisaKimura, Kazumitsu Ueda, Toru Nakatsu, and Hiroaki Kato
  • Organizer: The 5th Seoul-Kyoto-Osaka(SKO) Joint Symposium on Pharmaceutical Sciences for Young Scientist
  • Place of Presentation: Osaka, Japan