• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The serum glycoprotein/peptide analysis for discovery of novel biomarkers of hepatocellular carcinoma.

Research Project

Project/Area Number 22790521
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionChiba University

Principal Investigator

SOGAWA Kazuyuki  千葉大学, 医学部・附属病院, 寄附研究部門教員 (50436440)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsプロテオミクス / 糖タンパク質 / 糖ペプチド / 原発性肝細胞癌 / レクチンビーズ / MALDI-TOF MS / 二次元電気泳動 / N型糖タンパク質 / N型糖ペプチド / 血清 / プロテオーム解析
Research Abstract

Changes in glycosylation, most notably fucosylation, have been associated with the development of hepatocellular carcinoma (HCC). In this report, the levels of growth/differentiation facter 5 (GDF5) was analyzed individually with currently used marker, AFP and PIVKA-II, for the ability to distinguish between a diagnosis of cirrhosis and HCC. This analysis was done on serum from 50 patients with cirrhosis and 50 serum samples from patients with cirrhosis plus HCC. The levels of GDF5 was significantly higher in patients with HCC compared with those with cirrhosis (P<0.01).

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (23 results)

All 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (9 results) Book (1 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] HPLCとMALDI-TOF MSによる血清糖鎖欠損トランスフェリンの評価2012

    • Author(s)
      曽川一幸、飯田史枝、川島祐介、山田真子、佐藤 守、角谷真知子、瀧澤弘隆、丸山勝也、和田芳直、野村文夫
    • Journal Title

      アルコールと医学生物学

      Volume: 31(In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased serum levels of pigment epithelium-derived factor by excessive alcohol consumption-Detection and identification by a three-step serum proteome analysis-2011

    • Author(s)
      Sogawa K, Kodera Y, Satoh M, Kawashima Y, Umemura H, Maruyama K, Takizawa H, Yokosuka O, Nomura F
    • Volume
      Vol.35、No.2
    • Pages
      211-217
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The measurement of a fibrinogen alpha C-chain 5.9 kDa fragment (FIC 5.9) using MALDI-TOF MS and a stable isotope-labeled peptide standard dilution、 Clinica Chimica Acta2011

    • Author(s)
      Sogawa K, Kodera Y, Noda K, Ishizuka Y, Yamada M, Umemura H, Maruyama K, Tomonaga T, Yokosuka O, Nomura F
    • Volume
      Vol.412 、No.11-12
    • Pages
      1094-1099
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fibrinogen alpha C-chain 5.9kDaフラグメント(FIC5.9)の線維化マーカーとしての評価2011

    • Author(s)
      曽川一幸, 野田健太, 梅村啓史, 清宮正徳, 金井文彦, 今関文夫, 瀧澤弘隆, 横須賀收, 野村文夫
    • Journal Title

      医学と薬学

      Volume: 66巻 Pages: 329-337

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fibrinogen alpha C-chain 5.9 kDaフラグメント(FIC5.9)の線維化マーカーとしての評価2011

    • Author(s)
      曽川一幸、野田健太、梅村啓史、清宮正徳、金井文彦、今関文夫、瀧澤弘隆、横須賀收、野村文夫
    • Journal Title

      医学と薬学

      Volume: 66 Pages: 329-337

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The measurement of a fibrinogen alpha C-chain 5.9 kDa fragment (FIC 5.9)using MALDI-TOF MS and a stable isotope-labeled peptide standard dilution2011

    • Author(s)
      Sogawa K, Kodera Y, Noda K, Ishizuka Y, Yamada M, Umemura H, Maruyama K, Tomonaga T, Yokosuka O, Nomura F
    • Journal Title

      Clinica Chimica Acta

      Volume: 412 Issue: 11-12 Pages: 1094-1099

    • DOI

      10.1016/j.cca.2011.02.029

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Use of the MALDI BioTyper^<TM> system with MALDI-TOF mass spectrometry for the rapid identification of microorganisms2011

    • Author(s)
      Sogawa K, Watanabe M, Sato K, Segawa S, Ishii C, Miyabe A, Murata S, Saito T, Nomura F
    • Journal Title

      Analytical and bioanalytical chemistry

      Volume: 400 Issue: 7 Pages: 1905-1911

    • DOI

      10.1007/s00216-011-4877-7

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased serum levels of pigment epithelium-derived factor by excessivealcohol consumption -Detection and identification by a three-step serumproteome analysis-2011

    • Author(s)
      Sogawa K, Kodera Y, Satoh M, Kawashima Y, Umemura H, Maruyama K, Takizawa H, Yokosuka O, Nomura F.
    • Journal Title

      Alcoholism Clinical and Experimental Research

      Volume: 35 Pages: 211-217

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] HPLC とMALDI-TOF MSによる血清糖鎖欠損トランスフェリンの評価2012

    • Author(s)
      曽川一幸, 飯田史枝, 佐藤守, 川島祐介, 山田真子, 角谷真知子, 丸山勝也, 和田芳直, 野村文夫
    • Organizer
      第31回アルコール医学生物学研究会学術集会
    • Place of Presentation
      ホテル日航金沢
    • Year and Date
      2012-01-28
    • Related Report
      2011 Final Research Report
  • [Presentation] HPLCとMALDI-TOF MSによる血清糖鎖欠損トランスフェリンの評価2012

    • Author(s)
      曽川一幸、飯田史枝、川島祐介、山田真子、佐藤 守、角谷真知子、瀧澤弘隆、丸山勝也、和田芳直、野村文夫
    • Organizer
      第31回アルコール医学生物学研究会学術集会
    • Place of Presentation
      金沢(ホテル日航金沢)
    • Year and Date
      2012-01-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 質量分析計を用いた細菌迅速同定法の臨床応目指して-Biotyperを利用した臨床検査現場からの報告-2011

    • Author(s)
      曽川一幸、渡邊正治、野村文夫
    • Organizer
      第36回日本医用マススペクトル学会年会
    • Place of Presentation
      大阪(ホテル阪急エキスポパーク)(招待講演)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 血清糖鎖欠損トランスフェリンの高感度測定を目指して2010

    • Author(s)
      曽川一幸, 飯田史枝, 佐藤守, 川島祐介, 山田真子, 角谷真知子, 丸山勝也, 和田芳直, 野村文夫
    • Organizer
      第30回アルコール医学生物学研究会学術集会
    • Place of Presentation
      久留米翠香園ホテル
    • Year and Date
      2010-11-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Measurement of 5.9 kDa fragment of fibrinogen alpha-C chain (FIC 5.9) by MALDI-TOF MS with stable-isotope labeled standard in patients with liver diseases2010

    • Author(s)
      Sogawa K, Kodera Y, Noda K, Umemura H, Matsushita K, Kojima R, Katayama K, Nomura F
    • Organizer
      HUPO 9th Annual World Congress
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2010-09-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Measurement of 5.9 kDa fragment of fibrinogen alpha-C chain (FIC 5.9) by MALDI-TOF MS with stable-isotope labeled standard in patients with liver diseases.2010

    • Author(s)
      Sogawa K, Kodera Y, Noda K, Umemura H, Matsushita K, Kojima R, Katayama K, Nomura F.
    • Organizer
      HUPO 9th Annual World Congress
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 消化器領域における多戦略的疾患プロテオミクス2010

    • Author(s)
      曽川一幸、高野重紹、清宮正徳、佐藤守、小寺義男、野村文夫
    • Organizer
      第57回日本臨床検査医学会学術集会
    • Place of Presentation
      東京(京王プラザホテル)
    • Year and Date
      2010-09-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 消化器領域における多戦略的疾患プロテオミクス2010

    • Author(s)
      曽川一幸, 高野重紹, 清宮正徳, 佐藤守, 小寺義男, 野村文夫
    • Organizer
      第57回日本臨床検査医学会学術集会
    • Place of Presentation
      京王プラザホテル
    • Year and Date
      2010-08-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 質量分析装置を用いた問題飲酒者診断法確立への基礎的検討2010

    • Author(s)
      曽川一幸, 野田健太, 小島良, 野村文夫
    • Organizer
      第59回日本医学検査学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-05-23
    • Related Report
      2011 Final Research Report
  • [Book] アルコールと医学生物学2010

    • Author(s)
      曽川一幸, 他
    • Publisher
      東洋書店
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.m.chiba-u.ac.jp/class/moldiag/clinical_proteomics_index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.m.chiba-u.ac.jp/class/moldiag/clinical_proteomics_index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.m.chiba-u.ac.jp/class/moldiag/clinical_proteomics_index.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] コフィリンとその自己抗体との複合体の免疫測定方法、それに用いるキットおよびそれを用いた癌判定方法2010

    • Inventor(s)
      曽川一幸, 小島良, 野田健太, 宮崎勝, 吉富秀幸, 高野重紹, 野村文夫, 佐藤守
    • Industrial Property Rights Holder
      曽川一幸, 小島良, 野田健太, 宮崎勝, 吉富秀幸, 高野重紹, 野村文夫, 佐藤守
    • Filing Date
      2010-05-28
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] Novel Biomarkers of Biliary Tract Cancer2010

    • Inventor(s)
      曽川一幸, 吉川真太郎, 横須賀收, 野村文夫
    • Industrial Property Rights Holder
      曽川一幸, 吉川真太郎, 横須賀收, 野村文夫
    • Filing Date
      2010-11-05
    • Related Report
      2011 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi