| Project/Area Number |
22790522
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Okayama University (2011)
Chiba University (2010) |
|---|
Principal Investigator |
|
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | プロテオーム / 癌 / 臨床検査 / 自己抗体 / 腫瘍マーカー |
|---|
| Research Abstract |
We constructed a novel proteomic analysis system for the detecting of autoantibodies from sera. This system is composed of first screening procedure of comparative western blotting and subsequent mass analysis. At first, we extracted proteins from four poorly-differenciated gastric cancer cell lines. Using comprehensive western blotting in which protein extracts were used as antigen, we analysed sera from gastric cancer patients and healthy controls. In this analysis, we detected several bands, and identified thirty proteins from these bands with mass spectrometry. Among them, two proteins were successfully validated. These proteins were considered to be novel biomarkers for poorly-differnciated gastric cancer
|
