New drug sensitivity test for leukemia mimicking bone marrow microenvironment in hypoxia
Project/Area Number |
22790523
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Laboratory medicine
|
Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
ITOH Mai 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (70415545)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 臨床血液学 / 白血病 / 低酸素 / 骨髄環境 / 低酸素培養 / 白血病細胞 / 薬剤感受性試験 / 自己複製能 |
Research Abstract |
For treatment of leukemia, it is necessary to assess antileukemia agent by precise drug sensitivity test. We assumed that cell culture system in hypoxia mimicking bone marrow microenvironment is useful for assess the effect than the system in normoxia. In this study, we found that hypoxia culture system upregurated HIF protein expression as well as affected Notch, NF-kB, and mTOR signaling in leukemia stem cells. We established a basis for a new drug sensitivity test in hypoxia.
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Report
(4 results)
Research Products
(26 results)