Study on the mechanism of hypertension via endogenous digitalis-like factor in central nervous system

• Project/Area Number: 22790534
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Kansai Medical University
• Principal Investigator: YOSHIKA Masamichi 関西医科大学, 医学部, 助教 (70434834)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 内因性ジギタリス / 高血圧 / 生理活性物質 / 質量分析 / 脳 / 神経 / 視床下部 / 副腎 / 培養細胞 / アルドステロン / ELISA / HPLC

Research Abstract

Endogenous digitalis-like factor(EDLF) exist in the central nerve system as well as peripheral tissue. Ouabain is existed in hypothalamus and regulated by renin angiotensin aldosterone system(RAAS). A novel EDLF belonged to bufadienolide may be secreted from hypothalamus immortalized cell line. Moreover angiotensin should regulate to secret marinobufagenin and marinobufotoxin from adrenal gland.

Research Products

• [Journal Article] An ouabain-like factor is secreted from immortalized hypothalamic cells in an aldosterone-dependent manner (2011)
  • Author(s): Yoshika M, Komiyama Y, Takahashi H
  • Journal Title: Neurochem Int Volume: 59 Pages: 104-8
  • Peer Reviewed
• [Journal Article] The central mechanism underlying hypertension : a review of the roles of sodium ions, epithelial sodium channels, the renin-angiotensin-aldosterone system, oxidative stress and endogenous digitalis in the brain (2011)
  • Author(s): Takahashi H, Yoshika M, Komiyama Y, Nishimura M
  • Journal Title: Hypertens Res Volume: 34 Pages: 1147-60
  • NAID: 10030276766
  • Peer Reviewed
• [Journal Article] Isolation of marinobufotoxin from the supernatant of cultured PC12 cells (2011)
  • Author(s): Yoshika M, Komiyama Y, Takahashi H
  • Journal Title: Clin Exp Pharmacol Physiol Volume: 38 Pages: 344-7
  • Peer Reviewed
• [Journal Article] Ca2+entry mode of Na+/Ca2+exchanger as a new therapeutic target for heart failure with preserved ejection fraction (2011)
  • Author(s): Kamimura D, Ohtani T, Sakata Y, Komiyama Y, Yoshika M, Takahashi H, Komuro I, Yamamoto K, et al
  • Journal Title: Eur Heart J
  • Peer Reviewed
• [Journal Article] 内因性ジギタリス (2011)
  • Author(s): 吉賀正亨, 小宮山豊, 高橋伯夫
  • Journal Title: 検査と技術 Volume: 39 Pages: 561-564
• [Journal Article] The central mechanism underlying hypertension ; a review of the roles of sodium ions, epithelial sodium channels, the renin-angiotensin-aldosterone system, oxidative stress and endogenous digitalis in the brain (2011)
  • Author(s): Takahashi H, Yoshika M, Komiyama Y, Nishimura M
  • Journal Title: Hypertens Res Volume: 34 Issue: 11 Pages: 1147-1160
  • DOI: 10.1038/hr.2011.105
  • NAID: 10030276766
  • Peer Reviewed
• [Journal Article] An ouabain-like factor is secreted from immortalized hypothalamic_cells in an aldosteroue-dependent manner (2011)
  • Author(s): Yoshika M, Komiyama Y, Takahashi H
  • Journal Title: Neurochem Int Volume: 59 Issue: 2 Pages: 104-108
  • DOI: 10.1016/j.neuint.2011.04.019
  • Peer Reviewed
• [Journal Article] Ca2+ entry mode of Na+/Ca2+ exchanger as a new therapeutic target for heart failure with preserved ejection fraction (2011)
  • Author(s): Kamimura D, Komiyama Y, et al
  • Journal Title: Eur Heart J Volume: (Epub) Issue: 11 Pages: 1-9
  • DOI: 10.1093/eurheartj/ehr106
  • Peer Reviewed
• [Journal Article] An ouabain-like factor is secreted from immortalized hypothalamic cells in an aldosterone-dependent manner (2011)
  • Author(s): Yoshika M, Komiyama Y, Takahashi H
  • Journal Title: Neurochemistry International Volume: (in press)
  • Peer Reviewed
• [Journal Article] Ca2^+ entry mode of Na^+/Ca^<2+> exchanger as a new therapeutic target for heart failure with preserved ejection fraction (2011)
  • Author(s): Kamimura D, Sakata Y, Komiyama Y, Yoshika M, Takahashi H, Yamamoto K., et.al.
  • Journal Title: European Heart Journal Volume: (in press)
  • Peer Reviewed
• [Journal Article] Isolation of marinobufotoxin from the supernatant of cultured PC12 cells. (2011)
  • Author(s): Yoshika M, Komiyama Y, Takahashi H
  • Journal Title: Clinical and Experimental Pharmacology and Physiology Volume: 38 Pages: 334-337
  • Peer Reviewed
• [Presentation] 視床下部由来細胞株を用いた内因性ジギタリス様物質であるウアバイン分泌に関する検討 (2011)
  • Author(s): 吉賀正亨, 小宮山豊, 高橋伯夫
  • Organizer: 第9回腎と高血圧Update
  • Place of Presentation: 東京
  • Year and Date: 2011-12-03
• [Presentation] 視床下部由来細胞株を用いた内因性ジギタリス様物質であるウアバイン分泌に関する検討 (2011)
  • Author(s): 吉賀正亨, 小宮山豊, 高橋伯夫
  • Organizer: 第9回腎と高血圧Update
  • Place of Presentation: 品川プリンスホテル(東京)
  • Year and Date: 2011-12-03
• [Presentation] 新規内因性ジギタリスであるtelocinobufotoxinの昇圧作用の検討 (2011)
  • Author(s): 吉賀正亨, 小宮山豊, 高橋伯夫
  • Organizer: 第15回日本心血管内分泌代謝学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-26
• [Presentation] 新規内因性ジギタリスであるtelocinobufotoxinの昇圧作用の検討 (2011)
  • Author(s): 吉賀正亨, 小宮山豊, 高橋伯夫
  • Organizer: 第15回日本心血管内分泌代謝学会学術総会
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 2011-11-26
• [Presentation] 高血圧発症機序における内因性ジギタリスの臨床検査学的研究 (2011)
  • Author(s): 吉賀正亨, 小宮山豊, 高橋伯夫
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2011-11-18
• [Presentation] 高血圧発症機序における内因性ジギタリスの臨床検査学的研究 (2011)
  • Author(s): 吉賀正亨, 小宮山豊, 高橋伯夫
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山コンベンションセンター(岡山)
  • Year and Date: 2011-11-18
• [Presentation] 副腎細胞を用いたアンジオテンシン2受容体拮抗薬の内因性ジギタリス分泌に関する検討 (2011)
  • Author(s): 吉賀正亨, 小宮山豊, 高橋伯夫
  • Organizer: 第51回日本臨床化学会年次学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2011-08-27
• [Presentation] 副腎細胞を用いたアンジオテンシン2受容体拮抗薬の内因性ジギタリス分泌に関する検討 (2011)
  • Author(s): 吉賀正亨, 小宮山豊, 高橋伯夫
  • Organizer: 第51回日本臨床化学会年次学術集会
  • Place of Presentation: 札幌医科大学(札幌)
  • Year and Date: 2011-08-27
• [Presentation] 中枢神経系における内因性ジギタリス様物質であるOuabain分泌に関する検討 (2010)
  • Author(s): 吉賀正亨, 小宮山豊, 高橋伯夫
  • Organizer: 第33回日本高血圧学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2010-10-15
• [Presentation] EVIDENCE FOR A MARINOBUFAGENIN-LIKE IMMUNOREACTIVITY IN CENTRAL NERVOUS SYSTEM (2010)
  • Author(s): Masamichi Yoshika, Yutaka Komiyama, Hakuo Takahashi
  • Organizer: 14th International SHR Symposium Montreal
  • Place of Presentation: Canada
  • Year and Date: 2010-09-24
• [Presentation] EVIDENCE FOR A MARINOBUFAGENIN-LIKE IMMUNOREACTIVITY IN CENTRAL NERVOUS SYSTEM (2010)
  • Author(s): Masamichi Yoshika, Yutaka Komiyama, Hakuo Takahashi
  • Organizer: 14^<th> International SHR Symposium
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2010-09-24
• [Presentation] 高血圧発症機序における内因性ジギタリスの臨床検査学的研究 (2010)
  • Author(s): 吉賀正亨, 小宮山豊, 高橋伯夫
  • Organizer: 第57回日本臨床検査医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-10
• [Remarks]
  • URL: http://research.kmu.ac.jp/kmuhp/KgApp?kyoinId=ygdgbe
• [Remarks]
  • URL: http://research.kmu.ac.jp/kmuhp/KgApp?kyoinId=ygdgbe