Male contraceptive vaccine using the sperm and the spermatid in mice

• Project/Area Number: 22790572
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Public health/Health science
• Research Institution: Tokyo Medical University
• Principal Investigator: NAITO Munekazu 東京医科大学, 医学部, 講師 (10384984)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 精巣 / 精子形成 / 男性不妊 / 人口爆発 / ワクチン / 避妊ワクチン / 自己免疫 / 精子形成障害 / 自己抗原 / 炎症 / 自己抗体 / 精巣血液関門 / 男性不妊症 / 人口問題

Research Abstract

Experimental autoimmune orchitis(EAO) is a model of immunological inflammation demonstrating development of male infertility. We tried to develop the male contraceptive vaccine using murine EAO model induced by the immunization of sperm and spermatid antigens. The results showed that five kinds of protein from candidating 18 genes were adequate for induction of EAO. Moreover, we clarified the pathology of post-active inflammation stage of EAO. These results lead to the development of male contraceptive vaccine.

Research Products

• [Journal Article] Multiple renal vessels associated with testicular vessels (2011)
  • Author(s): Naito M, Terayama H, Hirai S, Ning Q, Yi SQ, Miyaki T, Itoh M
  • Journal Title: Surgical and Radiologic Anatomy Volume: 33(7) Pages: 637-9
• [Journal Article] Experimental model of autoimmune orchitis with abdominal placement of donor's testes, epididymes and vasa deferentia in recipient mice. (2011)
  • Author(s): Terayama H, Itoh M, Naito M, Hirai S, Qu N, Kuerban M, Musha M
  • Journal Title: Journal of Reproductive Immunology Volume: 90(2) Pages: 195-201
• [Journal Article] Multilayered structure of the basal lamina of the tubuli recti in normal mice (2011)
  • Author(s): Tainosho S, Naito M, Hirai S, Terayama H, Qu N, Itoh M
  • Journal Title: Medical Molecular Morphology Volume: 44 Pages: 34-38
  • NAID: 10029036540
• [Journal Article] Combined treatment with bortezomib plus bafilomycin A1 enhances cytocidal effect along with induction of endoplasmic reticulum(ER) stress in U266 myeloma cells : Crosstalk among proteasome, autophagy-lysosome, and ER Stress (2011)
  • Author(s): Kawaguchi T, Miyazawa K, Moriya S, Ohtomoa T, Che X, Naito M, Itoh M, Tomoda A
  • Journal Title: International journal of oncology Volume: 33 Pages: 643-654
• [Journal Article] A left testicular artery arising from a middle mesenteric artery (2011)
  • Author(s): Naito M, Yi SQ, Terayama H, Hirai S, Qu N, Itoh M
  • Journal Title: Clinical anatomy Volume: 24 Pages: 266-267
• [Journal Article] Intra-testicular expression of mRNAs of both interferon γ and tumor necrosis factor α is significantly increased in experimental autoimmune orchitis in mice (2011)
  • Author(s): Terayama H, Naito M, Qu N, Hirai S, Kitaoka M, Ogawa Y, Itoh M
  • Journal Title: The journal of reproduction and development Volume: 57 Pages: 296-302
  • NAID: 10028275659
• [Journal Article] Post-inflammation stage of autoimmune orchitis induced by immunization with syngeneic testicular germ cells alone in mice (2011)
  • Author(s): Naito M
  • Journal Title: Medical Molecular Morphology Volume: (In press)
  • NAID: 10030573640
  • Peer Reviewed
• [Journal Article] Intra-testicular expression of mRNAs of both interferon γ and tumor necrosis factor α is significantly increased in experimental autoimmune or chitis in mice (2011)
  • Author(s): Terayama H
  • Journal Title: The journal of reproduction and development Volume: 57 Pages: 296-302
  • Peer Reviewed
• [Journal Article] Experimental model of autoimmune orchitis with abdominal placement of donor's testes, epididymes and vasa deferentia in recipient mice (2011)
  • Author(s): Terayama H
  • Journal Title: Journal of Reproductive Immunology Volume: 90 Pages: 195-201
  • Peer Reviewed
• [Journal Article] Xenogeneic and endogenous spermatogenesis following transplantation of rat germ cells into testes of immunocompetent mice (2011)
  • Author(s): Qu N
  • Journal Title: Reproduction, Fertility and Development Volume: (In press)
  • Peer Reviewed
• [Journal Article] Post-inflammation stage of autoimmune orchitis induced by immunization with syngeneic testicular germ cells alone in mice. (2011)
  • Author(s): Naito M
  • Journal Title: Medical Molecular Morphology Volume: (In press)
  • NAID: 10030573640
  • Peer Reviewed
• [Journal Article] Intra-testicular expression of mRNAs of both interferon γ and tumor necrosis factor α is significantly increased in experimental autoimmune or chitis in mice. (2011)
  • Author(s): Terayama H
  • Journal Title: The journal of reproduction and development Volume: (In press)
  • Peer Reviewed
• [Journal Article] The Changes of Cortactin p80/85 Isoform Profiles and Tyrosine Phosphorylation Status during Spermatogenesis in the Mouse Testis. (2011)
  • Author(s): Miyaso H
  • Journal Title: Journal of Andrology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Combined treatment with bortezomib plus bafilomycin A1 enhances cytocidal effect along with induction of endoplasmic reticulum (ER) stress in U266 myeloma cells : Crosstalk among proteasome, autophagy-lysosome, and ER Stress. (2011)
  • Author(s): Kawaguchi T
  • Journal Title: International journal of oncology Volume: (In press)
  • Peer Reviewed
• [Journal Article] A left testicular artery arising from a middle mesenteric artery. (2011)
  • Author(s): Naito M
  • Journal Title: Clinivcal anatomy Volume: (In press)
  • Peer Reviewed
• [Journal Article] Multilayered structure of the basal lamina of the tubuli recti in normal mice. (2011)
  • Author(s): Tainosho S
  • Journal Title: Medical Molecular Morphology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Multiple renal vessels associated with testicular vessels. (2011)
  • Author(s): Naito M
  • Journal Title: Journal of Andrology Volume: (In press)
  • Peer Reviewed
• [Journal Article] The Changes of Cortactin p80/85 Isoform Profiles and Tyrosine Phosphorylation Status during Spermatogenesis in the Mouse Testis (2010)
  • Author(s): Miyaso H, Komiyama M, Matsuno Y, Naito M, Hirai S, Itoh M, Mori C
  • Journal Title: Journal of Andrology Volume: 31 Pages: 507-518
• [Journal Article] Developmental ontogeny of autoantigens associated with localized autoimmunity in murine testis and epididymis (2010)
  • Author(s): Qu N, Naito M, Terayama H, Hirai S, Li J, Ogawa Y, Kitaoka M, Itoh M
  • Journal Title: Journal of Reproductive Immunology Volume: 87 Pages: 45-51
• [Journal Article] Distribution of ghrelin-producing cells in stomach and the effects of ghrelin administration in the house musk shrew(Suncus murinus) (2010)
  • Author(s): Li J, Yi SQ, Terayama H, Naito M, Hirai S, Qu N, Wang HX, Yi N, Ozaki N, Itoh M
  • Journal Title: Neuro Endocrinology Letters Volume: 31 Pages: 406-412
• [Journal Article] Difference in abundance of blood and lymphatic capillaries in the murine epididymis (2010)
  • Author(s): Hirai S, Naito M, Terayama H, Ning Q, Miura M, Itoh M
  • Journal Title: Medical Molecular Morphology Volume: 43 Pages: 37-42
  • NAID: 10026443189
• [Journal Article] Effects of splenectomy on spontaneously chronic pancreatitis in aly/aly mice (2010)
  • Author(s): Wang H, Yi S, Li J, Terayama H, Naito M, Hirai S, Ning Q, Yi N and Itoh M
  • Journal Title: Clinical and Developmental Immunology Pages: 614890-614890
• [Journal Article] Cytoprotective effect of imatinib mesylate in non-BCR-ABL-expressing cells along with autophagosome formation (2010)
  • Author(s): Ohtomo T, Miyazawa K, Naito M, Moriya S, Kuroda M, Itoh M, Tomoda A
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 391 Pages: 310-315
• [Journal Article] Post-inflammation stage of autoimmune orchitis induced by immunization with syngeneic testicular germ cells alone in mice
  • Author(s): Naito M, Hirai S, Terayama H, Qu N, Kuerban M, Musha M, Kitaoka M, Ogawa Y, Itoh M
  • Journal Title: Medical Molecular Morphology Volume: (in press)
  • NAID: 10030573640
• [Journal Article] Xenogeneic and endogenous spermatogenesis following transplantation of rat germ cells into testes of immunocompetent mice
  • Author(s): Qu N, Naito M, Li J, Terayama H, Hirai S, Itoh M
  • Journal Title: Reproduction, Fertility and Development Volume: (in press)
• [Journal Article] Preservation by Desiccation of Isolated Rat Hearts for 48 Hours using Carbon Monoxide(PCO=4, 000 hPa) and Oxygen(PO2=3, 000 hPa)
  • Author(s): Hatayama N, Naito M, Hirai S, Kozhima T, Seki K, Li X, Itoh M
  • Journal Title: Cell Transplant Volume: (in press)
• [Journal Article] Mucosal lymphatic vessels of the esophagus distant from the cancer margin : morphometrical analysis using 27 surgically removed specimens of squamous cell carcinoma located in the upper or middle thoracic esophagus
  • Author(s): Nishimori H, Hayashi S, Naito M, Murakami G, Fujita M, Hosokawa M
  • Journal Title: Okajimas Folia Anatomica Japonica Volume: (in press)
  • NAID: 130004773596
• [Journal Article] Autologus groin lymph node transfer for lymph network reconstruction after head-and-neck cancer resection and neck lymph node dissection : a case report
  • Author(s): Mihara M, Hayashi Y, Iida T, Moriguchi H, Hara H, Yamamoto T, Narushima M, Todokoro T, Uchida G, Hayami S, Sawamoto N, Naito M, Koshima I
  • Journal Title: Journal of Plastic, Reconstructive & Aesthetic Surgery Volume: (in press)
• [Presentation] 自己免疫性精子形成障害における微小環境の解析 (2011)
  • Author(s): 内藤宗和
  • Organizer: 第43回日本臨床分子形態学会総会・学術集会
  • Place of Presentation: 大阪大学
  • Year and Date: 2011-09-10
• [Presentation] Autoimmune orchitis is one of main causes of idiopathic male infertility? (2011)
  • Author(s): Munekazu Naito, Shuichi Hirai, Hayato Terayama, Ning Qu, Maimaiti Kuerban, Muhetaerjiang Musha, Yuki Ogawa, Masahiro Itoh
  • Organizer: 第167回東京医科大学医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-04
• [Presentation] Autoimmune orchitis is one of main causes of idiopathic male infertility? (2011)
  • Author(s): 内藤宗和
  • Organizer: 第167回東京医科大学医学会総会
  • Place of Presentation: 東京医科大学
  • Year and Date: 2011-06-04
• [Presentation] 自己免疫性精子形成障害における微小環境の解析 (2011)
  • Author(s): 内藤宗和
  • Organizer: 第43回日本臨床分子形態学会総会・学術集会
  • Place of Presentation: 大阪
• [Presentation] Pathology of post-inflammation stage of autoimmune orchitis induced by immunization with syngeneic testicular germ cells alone inmice (2011)
  • Author(s): Munekazu Naito, Shuichi Hirai, Hayato Terayama, Ning Qu, Masahiro Itoh
  • Organizer: 6th European Congress of Andrology
  • Place of Presentation: Greece
• [Presentation] Autoimmune orchitis is one of main causes of idiopathic male infertility? (2010)
  • Author(s): Munekazu Naito
  • Organizer: 第25回日本生殖免疫学会総会・学術集会
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-08-29
• [Presentation] ヒト特発性精子形成障害と類似した病理像を呈するマウス自己免疫性精巣炎の後期像 (2010)
  • Author(s): 内藤宗和
  • Organizer: 日本アンドロロジー学会第29回学術大会ならびに総会および第16回精子形成・精巣毒性研究会共同開催学会
  • Place of Presentation: 日本獣医生命科学大学
  • Year and Date: 2010-07-30
• [Presentation] Autoimmune orchitis is one of main causes of idiopathic male infertility? (2010)
  • Author(s): Munekazu Naito, Shuichi Hirai, Hayato Terayama, Ning Qu, Masahiro Itoh
  • Organizer: International Symposium for Immunology of Reproduction/25th Annual meeting of Japan Society for Immunology of Reproduction, Joint Meeting
  • Place of Presentation: 大阪
• [Presentation] ヒト特発性精子形成障害と類似した病理像を呈するマウス自己免疫性精巣炎の後期像 (2010)
  • Author(s): 内藤宗和, 平井宗一, 寺山隼人, 曲寧, 北岡三幸, 小川夕輝, 伊藤正裕
  • Organizer: 日本アンドロロジー学会第29回学術大会ならびに総会および第16回精子形成・精巣毒性研究会共同開催学会
  • Place of Presentation: 東京
• [Book] スンクスの生物学 (2011)
  • Author(s): 内藤宗和, 平井宗一, 寺山隼人, 伊藤正裕, 九郎丸正道, 木曽康郎
  • Publisher: 学会出版センター
• [Book] 「スンクスの生物学」IV-5:生殖器 (2011)
  • Author(s): 内藤宗和
  • Publisher: 学会出版センター
• [Book] スンクスの生物学 (2011)
  • Author(s): 内藤宗和
  • Total Pages: 4
  • Publisher: 学会出版センター
• [Remarks]
  • URL: http://www.tokyo-med.ac.jp/anatomy/index.html
• [Remarks]
  • URL: http://www.tokyo-med.ac.jp/anatomy/index.html
• [Remarks]
  • URL: http://www.tokyo-med.ac.jp/anatomy/index.html