Comprehension of epidemiologic information and genomics of Mycobacterium tuberculosis clinical strains causing putative nationwide proliferation

• Project/Area Number: 22790585
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Public health/Health science
• Research Institution: Osaka City Institute of Public Health and Environmental Sciences
• Principal Investigator: WADA Takayuki 大阪市立環境科学研究所, 研究主任 (70332450)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
• Keywords: 感染症 / ゲノム / 衛生 / 社会医学 / 分子疫学 / 公衆衛生 / 結核 / 細菌 / ゲノム比較

Research Abstract

Genotyping of clinical isolates of Mycobacterium tuberculosis has been used to estimate their putative transmission routes. In this study, we focused on two genotypes of M. tuberculosis, pECT04 and pECT07, detected occasionally from broad areas in Japan. Totally twelve clinical isolates of the genotypes were genomically sequenced for comparative analysis. Results show that phylogenetic relation among pECT07 isolates was not associated with their geographic origins, which suggests their complicated transmission routes around Japan. Contrary to pECT04, many SNPs characteristic to two isolates of pECT04 were identified. These results mean that comparative genomics of clinical isolates can provide deep traceability of transmission of M. tuberculosis that has never achieved with present genotyping methods.

Research Products

• [Presentation] ゲノム比較に基づく結核菌臨床分離株の遺伝的多様性包囲網の構築 (2012)
  • Author(s): 和田崇之,岩本朋忠,長谷篤,前田伸司
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] ゲノム比較に基づく結核菌臨床分離株の遺伝的多様性包囲網の構築 (2012)
  • Author(s): 和田崇之、岩本朋忠, 他
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎)
• [Presentation] わが国における北京型結核菌の集団構造に即したVNTR型別データの考え方 (2011)
  • Author(s): 和田崇之,岩本朋忠
  • Organizer: 第85回日本感染症学会総会
  • Place of Presentation: 東京
• [Presentation] ホームレス結核患者由来株のVNTR型別解析:多発性大規模伝播と未知の伝播経路推定 (2011)
  • Author(s): 和田崇之,小向潤,有馬和代,團野桂,松本健二,吉田英樹,加藤仁一,岩本朋忠,田丸亜貴,吉田志緒美,下内昭,長谷篤
  • Organizer: 第86回日本結核病学会総会
  • Place of Presentation: 東京
• [Presentation] Characterization of genomic specificity and diversity observed in Mycobacterium tuberculosis in far-eastern Asia (2011)
  • Author(s): Takayuki Wada, Tomotada Iwamoto, Atsushi Hase, and Shinji Maeda
  • Organizer: FEMS Microbiology Congress 2011
  • Place of Presentation: Geneva
• [Presentation] わが国における北京型結核菌の集団構造に即したVNTR型別データの考え方 (2011)
  • Author(s): 和田崇之、岩本朋忠
  • Organizer: 第85回日本感染症学会総会
  • Place of Presentation: パークタワー東京(東京)
• [Presentation] ホームレス結核患者由来株のVNTR型別解析:多発性大規模伝播と未知の伝播経路推定 (2011)
  • Author(s): 和田崇之、小向潤, 他
  • Organizer: 第86回日本結核病学会総会
  • Place of Presentation: 日本教育会館(東京)
• [Presentation] Characterization of genomic specificity and diversity observed in Mycobacterium tuberculosis in far-eastern Asia (2011)
  • Author(s): Wada T, Iwamoto T, et al
  • Organizer: FEMS Microbiology Congress 2011
  • Place of Presentation: Plexpo Geneva Congress Center (Switzerland)