Individual identification by discrimination of mismatch nucleotides using RNase A

Research Project

Project/Area Number	22790592
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Legal medicine
Research Institution	Asahikawa Medical College
Principal Investigator	ASARI Masaru 旭川医科大学, 医学部, 助教 (40360979)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	個人識別 / 一塩基多型 / RNA分解酵素A
Research Abstract	In 261G/deletion and 796C/A on the human ABO gene, we could genotype the SNP alleles based on the mismatches between template-probe duplexes using synthesized RNA, DNA/RNA chimeric probes, and RNase A. The lengths of probes and the positions of RNA bases corresponding to the SNP affected the sensitivity and specificity of genotyping. Furthermore, the genotypes in two loci were identified by probes designed with the different sizes.