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Detection of diverse aquatic microbes in blood and organs of drowning victims by a metagenomic approach

Research Project

Project/Area Number 22790598
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Legal medicine
Research InstitutionUniversity of Miyazaki

Principal Investigator

KAKIZAKI Eiji  宮崎大学, 医学部, 助教 (70284833)

Research Collaborator YUKAWA Nobuhiro  宮崎大学, 医学部, 教授 (30240154)
KOZAWA Shuji  宮崎大学, 医学部, 助教 (20379944)
HAYASHI Tetsuya  宮崎大学, フロンティア科学実験総合センター, 教授 (10173014)
OGURA Yoshitoshi  宮崎大学, フロンティア科学実験総合センター, 助教 (40363585)
UCHIYAMA Taketo  宮崎大学, 大学院・医学系研究科, 大学院生
NISHIDA Sho  宮崎大学, 大学院・医学獣医学総合研究科, 大学院生
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords法医学 / 溺死 / 水棲微生物 / プランクトン / 水棲細菌 / 珪藻 / 藍藻
Research Abstract

We used a high-throughput 454-pyrosequencing strategy to detect diverse aquatic microbes in the blood and organs of two drowning victims and obtained novel information about various microbes. This technique was very useful for determining the diversity of aquatic microbes such as bacteria including cyanobacteria, diatoms and other algae in drowning victims. This technique therefore has high potential as a novel molecular tool with which to examine whether an immersed victim has drowned, especially when the results of conventional diatom testing or other useful approaches are equivocal.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (5 results)

All 2012 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] Detection of diverse aquatic microbes in blood and organs of drowning victims : First metagenomic approach using high-throughput 454-pyrosequencing2012

    • Author(s)
      Eiji Kakizaki, Yoshitoshi Ogura, Shuji Kozawa, Sho Nishida, Taketo Uchiyama, Tetsuya Hayashi, Nobuhiro Yukawa
    • Journal Title

      Forensic Science International

      Volume: (in press) Pages: 10-10

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of diverse aquatic microbes in blood and organs of drowning victims : First metagenomic approach using high-throughput 454-pyrosequencing2012

    • Author(s)
      Kakizaki E, et al
    • Journal Title

      Forensic Science International

      Volume: (in press) Issue: 1-3 Pages: 135-146

    • DOI

      10.1016/j.forsciint.2012.02.010

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 水中死体の血中及び臓器中に存在する水棲微生物のメタゲノム解析:ピロシーケンス法を用いた群集構造解析2011

    • Author(s)
      柿崎英二, 小澤周二, 今村奈穂子, 湯川修弘
    • Organizer
      第95次日本法医学会総会
    • Place of Presentation
      コラッセふくしま(福島)
    • Year and Date
      2011-06-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 水中死体の血中及び臓器中に存在する水棲微生物のメタゲノム解析:ピロシーケンス法を用いた群集構造解析2011

    • Author(s)
      柿崎英二, ら
    • Organizer
      第95次日本法医学会学術全国集会
    • Place of Presentation
      コラッセふくしま(福島)
    • Year and Date
      2011-06-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 河口周辺水域で発見された水中死体8例における水棲微生物(珪藻・水棲細菌)の種類と溺死した水域の推定2010

    • Author(s)
      柿崎英二, 小澤周二, 内山岳人, 湯川修弘
    • Organizer
      第94次日本法医学会学術全国集会
    • Place of Presentation
      タワーホール船堀(東京)
    • Year and Date
      2010-06-24
    • Related Report
      2011 Final Research Report 2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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