The effect of kakkonto in allergic immune response mediated by dendritic cells on intestinal mucosal immune system

• Project/Area Number: 22790616
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: University of Toyama
• Principal Investigator: YAMAMOTO Takeshi 富山大学, 和漢医薬学総合研究所, 助教 (70316181)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 東洋医学 / 葛根湯 / 腸管粘膜免疫 / 食物アレルギー / 制御性細胞 / 樹状細胞 / 腸管粘膜免疫系 / 制御性T細胞

Research Abstract

Kakkonto, a traditional Japanese herbal medicine, suppressed the occurrence of allergic symptoms in an FA mouse model. Furthermore, Puerariae radix extract and several isoflavones suppressed the occurrence of allergic symptoms in an FA mouse model. The proportion of CD4+Foxp3+regulatory T cells in lamina propria was significantly increased bykakkonto treatment and isoflavones treatment as compared with FA mouse model. Our data suggest that CD4+Foxp3+regulatory T cells induced by kakkonto and isoflavones had suppressive effects on intestinal mucosal immunity in FA mice.

Research Products

• [Journal Article] Oral Tolerance Induced by Transfer of Food Antigens via Breast Milk of Allergic Mothers Prevents Offspring from Developing Allergic Symptoms in a Mouse Food Allergy Model (2012)
  • Author(s): Yamamoto T, Tsubota Y, Kodama T, Kageyama-Yahara N, Kadowaki M
  • Journal Title: Clinical and Developmental Immunology Volume 2012(2012) Volume: 2012 Pages: 721085-721085
  • DOI: 10.1155/2012/721085
  • Peer Reviewed
• [Journal Article] Sphingosine kinase-1-dependent and -independent inhibitory effects of zanthoxyli fructus to attenuate the activation of mucosal mast cells and ameliorate food allergies in mice (2012)
  • Author(s): Wang X, Kageyama-Yahara N, Hayashi S, Yamamoto T, Kadowaki M.
  • Journal Title: Evid Based Complement Alternat Med Volume: 2012 Pages: 862743-862743
  • DOI: 10.1155/2012/862743
  • Peer Reviewed
• [Journal Article] Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde in hibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells (2011)
  • Author(s): Kageyama-Yahara N, Wang X, Katagiri T, Wang P, Yamamoto T, Tominaga M, Kadowaki M
  • Journal Title: Biochem Biophys Res Commun Volume: 416 Issue: 3-4 Pages: 283-8
  • DOI: 10.1016/j.bbrc.2011.11.014
  • Peer Reviewed
• [Journal Article] Rab5a Regulates Surface Expression of Fc.EPSILON.RI and Functional Activation in Mast Cells (2011)
  • Author(s): Kageyama-Yahara N, Suehiro Y, Yamamoto T, Kadowaki M
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 34 Issue: 5 Pages: 760-763
  • DOI: 10.1248/bpb.34.760
  • NAID: 130000657792
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] The Inhibitory Effect of Ergosterol, a Bioactive Constituent of a Traditional Japanese Herbal Medicine Saireito on the Activity of Mucosal-Type Mast Cells (2010)
  • Author(s): Kageyama-Yahara N, Wang P, Wang X, Yamamoto T, Kadowaki M.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 33 Issue: 1 Pages: 142-145
  • DOI: 10.1248/bpb.33.142
  • NAID: 130000140209
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] Pentagalloylglucose down-regulates mast cell surface FcepsilonRI expression in vitro and in vivo (2010)
  • Author(s): Kageyama-Yahara N, Suehiro Y, Maeda F, Kageyama S, Fukuoka J, Katagiri T, Yamamoto T, Kadowaki M.
  • Journal Title: FEBS Lett. Volume: 4 Issue: 1 Pages: 111-118
  • DOI: 10.1016/j.febslet.2009.11.007
  • Peer Reviewed
• [Journal Article] Pentagalloylglucose down-regulates mast cell surface FcepsilonRI expression in vitro and in vivo. (2010)
  • Author(s): Kageyama-Yahara N, Suehiro Y, Maeda F, Kageyama S, Fukuoka J, Katagiri T, Yamamoto T, Kadowaki M.
  • Journal Title: FEBS Lett Volume: 584 Pages: 111-118
  • Peer Reviewed
• [Presentation] Kadowaki M. Shikonin suppresses the activation of mucosal mast cells through the inhibition of nuclear orphan receptors NR4A expression. (2012)
  • Author(s): Hayashi S., Wang X., Umezaki M., Yamamoto T.
  • Organizer: 5th International Symposium on Scientific Research of Traditional Medicine
  • Place of Presentation: 富山
  • Year and Date: 2012-10-13
• [Presentation] 葛根成分および代謝物による食物アレルギー性消化器症状の治療効果 (2012)
  • Author(s): 山本 武, 門脇 真
  • Organizer: 第29回和漢医薬学会学術大会.
  • Place of Presentation: 東京
• [Presentation] 紫雲膏の主要活性成分シコニンの核内オーファン受容体NR4Aの遺伝子発現抑制を介したマスト細胞に対する作用 (2012)
  • Author(s): 王 暁宇, 林 周作, 山本 武, 門脇 真
  • Organizer: 第29回和漢医薬学会学術大会
  • Place of Presentation: 東京
• [Presentation] 葛根湯の粘膜免疫系での制御性T細胞誘導による食物アレルギー抑制作用. (2012)
  • Author(s): 門脇 真, 山本 武
  • Organizer: 第16回日本統合医療学会(招待講演)
  • Place of Presentation: 大阪
• [Presentation] 葛根成分および代謝物による食物アレルギー性消化器症状の治療効果. (2012)
  • Author(s): 山本武，門脇真
  • Organizer: 第29回和漢医薬学会学術大会
  • Place of Presentation: 東京
• [Presentation] 紫雲膏の主要活性成分シコニンの核内オーファン受容体NR4Aの遺伝子発現抑制を介したマスト細胞に対する作用. (2012)
  • Author(s): 王暁宇，林周作，山本武，門脇真
  • Organizer: 第29回和漢医薬学会学術大会
  • Place of Presentation: 東京
• [Presentation] Shikonin suppresses the activation of mucosal mast cells through the inhibition of nuclear orphan receptors NR4A expression. (2012)
  • Author(s): Hayashi S., Wang X., Umezaki M., Yamamoto T., Kadowaki M.
  • Organizer: 5th International Symposium on Scientific Research of Traditional Medicine
  • Place of Presentation: 富山
• [Presentation] 葛根湯の粘膜免疫系での制御性T細胞誘導による食物アレルギー抑制作用. (2012)
  • Author(s): 門脇真，山本武
  • Organizer: 第16回日本統合医療学会
  • Place of Presentation: 大阪
  • Invited
• [Presentation] 樹状細胞を介した葛根湯によるエフェクターT細胞の分化・増殖の抑制 (2011)
  • Author(s): 山本 武, 原優 加, 門脇 真
  • Organizer: 第28回和漢医薬学会学術大会
  • Place of Presentation: 富山
• [Presentation] 樹状細胞を介した葛根湯によるエフェクターT細胞の分化・増殖の抑制 (2011)
  • Author(s): 山本武
  • Organizer: 第28回和漢医薬学会学術大会
  • Place of Presentation: 富山県民会館(富山県)
• [Presentation] 食物アレルギー病態モデルにおける葛根湯の新規制御性T細胞を介した腸管粘膜免疫抑制作用 (2010)
  • Author(s): 山本 武, 藤原 加苗, 坪田 悠真, 門脇真
  • Organizer: 第27回和漢医薬学会学術大会.
  • Place of Presentation: 京都
• [Presentation] 食物アレルギー病態モデルにおける葛根湯の新規制御性T細胞を介した腸管粘膜免疫抑制作用 (2010)
  • Author(s): 山本武
  • Organizer: 第27回和漢医薬学会学術大会
  • Place of Presentation: 京都