Development of new method for the three-dimensional analysis of transplanted islets

• Project/Area Number: 22790617
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: Kyoto University
• Principal Investigator: FUJIMOTO Hiroyuki 京都大学, 医学研究科, 助教 (50437274)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 代替医療 / 糖尿病 / 膵島移植 / OPT / 三次元解析 / イメージング

Research Abstract

I established a novel imaging technique suitable for analysis of transplanted islets in liver using an optical projection tomography(OPT) method. A three-dimensional tomographic image of the transplanted islets in liver was reconstructed. The number of islets transplanted and the number of transplanted islets observed using OPT showed good correlation. The OPT method was used to compare the numbers of transplanted islets in mouse syngeneic and allogeneic transplantation models. In mice transplanted with allogeneic islets, the number and the volume of transplanted islets was significantly reduced in hyperglycemic condition of allogeneic transplantation. These results indicate that OPT imaging and analysis may be a useful tool to quantitatively and sterically evaluation of transplanted islets in liver at the cellular level.

Research Products

• [Journal Article] Three-diiensional ex vivo imaging and analysis of intraportal islet transplants (2011)
  • Author(s): Fujimoto H, Toyoda K, Okitsu T, Liu X, Mukai E, Zhuang X, Uemoto S, Mochizuki N, Inagaki N
  • Journal Title: Transplant International Volume: 24 Issue: 8 Pages: 839-844
  • DOI: 10.1111/j.1432-2277.2011.01271.x
  • Peer Reviewed
• [Presentation] Non-Invasive SPECT Imaging of Pancreatic Islets Targeting Glucagon-Like Peptide-1 Receptors (2011)
  • Author(s): Fujimoto, H., Toyoda, K., Kimura, H., Ogawa, Y., Mukai, E., Xiaotohg, Z., Kawashima, H., Matsuda, H., Hirao, K., Saji, H., Inagaki, N
  • Organizer: American Diabetes Association 71th Scientific Sessions
  • Place of Presentation: サンディエゴ(アメリカ)
  • Year and Date: 2011-06-26
• [Presentation] Non-Invasive SPECT Imaging of Pancreatic Islets Targeting Glueagon-Like Peptide-1 Receptors (2011)
  • Author(s): Fujimoto, H., Toyoda, K., Kimura, H., Ogawa, Y., Mukai, E., Xiaotohg, Z., Kawashima, H, Matsuda, H., Hirao, K.,Saji, H., Inagaki, N.
  • Organizer: erican Diabetes Association 71th Scientific Sessio
  • Place of Presentation: San Diego, CA(アメリカ)
  • Year and Date: 2011-06-26
• [Presentation] Development of Non-Invasive PET Probe for Quantifying Pancreatic s-Cell Mass Using Fluorine-18-Labeled Exendin-4 (2011)
  • Author(s): Toyoda K, Kimura H, Fujimoto H, Xiaotong Z, Mukai E, Ogawa E, Hirao K, Matsuda H, Kawashima H, Ueda M, Temma T, Saji H and Inagaki N
  • Organizer: American Diabetes Association 71th Scientific Sessions
  • Place of Presentation: サンディエゴ(アメリカ)
  • Year and Date: 2011-06-25
• [Presentation] Development of Non-Invasive PET Probe for Quantifying Pancreatic β-Cell Mass Using Fluorine-18-Labeled Exendin-4 (2011)
  • Author(s): Toyoda K, Kimura H, Fujimoto H, Xiaotong Z, Mukai E, Ogawa E, Hirao K,Matsuda H, Kawashima H, Ueda M, Temma T, Saji H, Inagaki N
  • Organizer: erican Diabetes Association 71th Scientific Sessio
  • Place of Presentation: San Diego, CA(アメリカ)
  • Year and Date: 2011-06-25
• [Presentation] Radio synthesis and evaluation of F-18 Exendin-4 derivatives as a PET tracer for the imaging of GLP-1 receptors on pancreaticβ-cells (2011)
  • Author(s): Kimura H, Ogawa Y, Kawashima H, Toyoda K, Mukai E, Fujimoto H, Ono M, Hirao K, Inagaki N, Saji H
  • Organizer: SNM 2011 Annual Meeting
  • Place of Presentation: テキサス(アメリカ)
  • Year and Date: 2011-06-06
• [Presentation] Development of 123I labeled Exendin derivatives for the imaging of GLP-1 receptors on pancreaticβ-cell with SPECT (2011)
  • Author(s): Ogawa Y, Kimura H, Kawashima H, Toyoda K, Mukai E, Fujimoto H, Hirao K, Ono M, Inagaki N, Saji H
  • Organizer: SNM 2011 Annual Meeting
  • Place of Presentation: テキサス(アメリカ)
  • Year and Date: 2011-06-06
• [Presentation] Radiosynthesis and evaluation of F-18 Exendin-4 derivatives as a PET tracer for the imaging of GLP-1 receptors on pancreatic β-cells (2011)
  • Author(s): Kimura H, Ogawa Y, Kawashima H, Toyoda K,Mukai E, Fujimoto H, Ono M, Hirao K, Inagaki N, Saji H
  • Organizer: SNM 2011 Annual Meeting
  • Place of Presentation: SAN ANTONIO, TEXAS(アメリカ)
  • Year and Date: 2011-06-06
• [Presentation] Development of 1231 labeled Exendin derivatives for the imaging of GLP-1 receptors on pancreatic β-cell with SPECT (2011)
  • Author(s): Ogawa Y, Kimura H, Kawashima H, Toyoda K, Mukai E, Fujimoto H, Hirao K, Ono M, Inagaki N, Saji H
  • Organizer: SNM 2011 Annual Meeting
  • Place of Presentation: SAN ANTONIO, TEXAS(アメリカ)
  • Year and Date: 2011-06-06
• [Presentation] GLP-1受容体を標的とするSPECT用膵島イメージンググローブの開発 (2011)
  • Author(s): 藤本裕之、豊田健太郎、木村寛之、小川有、向英里、庄暁桐、河嶋秀和、平尾佳、松田洋和、佐治英郎、稲垣暢也
  • Organizer: 第53回日本糖尿病学会年次集会
  • Place of Presentation: 札幌
  • Year and Date: 2011-05-21
• [Presentation] マウス同系膵島移植において、ONO-1301はPGI2受容体を介する膵β細胞保護作用によって膵島生着を改善する (2011)
  • Author(s): 庄暁桐、豊田健太郎、劉喜宝、向英里、藤本裕之、酒井芳紀、古屋敷智之、松本邦夫、成宮周、稲垣暢也
  • Organizer: 第53回日本糖尿病学会年次集会
  • Place of Presentation: 札幌
  • Year and Date: 2011-05-21
• [Presentation] GLP-1受容体を標的とするSPECT用膵島イメージンググローブの開発 (2011)
  • Author(s): 藤本裕之、豊田健太郎、木村寛之、小川有、向英里、庄暁桐、河嶋秀和、平尾佳、松田洋和、佐治英郎、稲垣暢也
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌市教育文化会館(北海道)
  • Year and Date: 2011-05-21
• [Presentation] マウス同系膵島移植において、ONO-1301はPGI2受容体を介する膵β細胞保護作用によって膵島生着を改善する (2011)
  • Author(s): 庄暁桐、豊田健太郎、劉喜宝、向英里、藤本裕之、酒井芳紀、古屋敷智之、松本邦夫、成宮周、稲垣暢也
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌市教育文化会館(北海道)
  • Year and Date: 2011-05-21
• [Presentation] GLP-1受容体を標的とする非侵襲的膵島イメージングのためのPET用プローブの開発 (2011)
  • Author(s): 豊田健太郎、木村寛之、藤本裕之、小川佑、向英里、庄暁桐、河嶋秀和、平尾佳、松田洋和、佐治英郎、稲垣暢也
  • Organizer: 第53回日本糖尿病学会年次集会
  • Place of Presentation: 札幌
  • Year and Date: 2011-05-19
• [Presentation] GLP-1受容体を標的とする非侵襲的膵島イメージングのためのPET用プローブの開発 (2011)
  • Author(s): 豊田健太郎、木村寛之、藤本裕之、小川佑、向英里、庄暁桐、河嶋秀和、平尾佳、松田洋和、佐治英郎、稲垣暢也
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌市教育文化会館(北海道)
  • Year and Date: 2011-05-19
• [Presentation] 移植膵島の新規三次元イメージング技術の確立 (2010)
  • Author(s): 藤本裕之豊田健太郎向英里、庄暁桐稲垣暢也
  • Organizer: 第52回日本糖尿病学会年次集会
  • Place of Presentation: 岡山
  • Year and Date: 2010-05-29
• [Presentation] 移植膵島の新規三次元イメージング技術の確立 (2010)
  • Author(s): 藤本裕之
  • Organizer: 第52回日本糖尿病学会
  • Place of Presentation: 岡山 ラヴィール岡山
  • Year and Date: 2010-05-29