Identification and clinical application of cancer stem cell inhibitory microRNA

• Project/Area Number: 22790626
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Tohoku University
• Principal Investigator: HAMADA Shin 東北大学, 病院, 医員 (20451560)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: miR-126 / ADAM9 / EMT / 膵癌 / microRNA / 膵癌幹細胞 / マイクロRNA / 幹細胞マーカー

Research Abstract

Current study focused on cancer stem cell inhibitory microRNA. The microRNA expression profiles in invasive ductal adenocarcinoma (IDA)and IPMN were assessed by microarray and miR-126 was identified as a down-regulated microRNA in IDA. Normal pancreas tissue retained miR-126 expression while its expression was lost in IDA. ADAM9, a putative target of miR-126 was highly expressed in IDA, suggesting mutually exclusive pattern. The miR-126/ADAM9 axis could be a novel therapeutic target against pancreatic cancer stem cell.

Research Products

• [Journal Article] MiR-126 acts as a tumor suppressor in pancreatic cancer cells via the regulation of ADAM9 (2012)
  • Author(s): Hamada S, Satoh K, Fujibuchi W, Hirota M, Kanno A, Unno J, Masamune A, Kikuta K, Kume K, Shimosegawa T
  • Journal Title: Mol Cancer Res. Volume: 10(1) Pages: 3-10
  • Peer Reviewed
• [Journal Article] The homeobox gene MSX2 determines chemosensitivity of pancreatic cancer cells via the regulation of transporter gene ABCG2 (2012)
  • Author(s): Hamada S, Satoh K, Hirota M, Kanno A, Umino J, Ito H, Masamune A, Kikuta K, Kume K, Shimosegawa T.
  • Journal Title: J Cell Physiol. Volume: 227(2) Pages: 729-738
  • Peer Reviewed
• [Journal Article] MiR-126 acts as a tumor suppressor in pancreatic cancer cells via the regulation of ADAM9 (2012)
  • Author(s): Hamada S, Satoh K, Fujibuchi W, Hirota M, Kanno A, Unno J, Masamune A, Kikuta K, Kume K, Shimosegawa T
  • Journal Title: Mol Cancer Res Volume: 10(1) Issue: 1 Pages: 3-10
  • DOI: 10.1158/1541-7786.mcr-11-0272
  • Peer Reviewed
• [Journal Article] The homeobox gene MSX2 determines chemosensitivity of pancreatic cancer cells via the regulation of transporter gene ABCG2 (2012)
  • Author(s): Hamada S, Satoh K, Hirota M, Kanno A, Umino J, Ito H, Masamune A, Kikuta K, Kume K, Shimosegawa T
  • Journal Title: J Cell Physiol Volume: 227(2) Issue: 2 Pages: 729-738
  • DOI: 10.1002/jcp.22781
  • Peer Reviewed
• [Journal Article] Calcium-binding protein S100P is a novel diagnostic marker of cholangiocarcinoma (2011)
  • Author(s): Hamada S, Satoh K, Hirota M, Kanno A, Ishida K, Umino J, Ito H, Kikuta K, Kume K, Masamune A, Katayose Y, Unno M, Shimosegawa T.
  • Journal Title: Cancer Sci. Volume: 102(1) Pages: 150-156
  • NAID: 10029288859
  • Peer Reviewed
• [Journal Article] Calcium-binding protein S100P is a novel diagnostic marker of cholang iocarcinoma. (2011)
  • Author(s): Hamada S, Satoh K, Hirota M, Kanno A, Ishida K, Umino J, Ito H, Kikuta K, Kume K, Masamune A, Katayose Y, Unno M, Shimosegawa T.
  • Journal Title: Cancer Sci. Volume: 102(1) Pages: 150-156
  • NAID: 10029288859
  • Peer Reviewed
• [Journal Article] Evaluation of MSX2 mRNA in brush cytology specimens distinguished pancreatic carcinoma from chronic pancreatitis. (2011)
  • Author(s): Satoh K, Hamada S, Kanno A, Ishida K, Ito H, Hirota M, Masamune A, Egawa S, Unno M, Shimosegawa T.
  • Journal Title: Cancer Sci. Volume: 102(1) Pages: 157-161
  • NAID: 10029288885
  • Peer Reviewed
• [Presentation] 膵癌特異的なマイクロRNA発現プロファイル解析からの悪性化因子の同定 (2011)
  • Author(s): 濱田晋, 佐藤賢一, 下瀬川徹
  • Organizer: JDDW2011
  • Place of Presentation: 福岡
  • Year and Date: 2011-10-23
• [Presentation] 膵癌特異的なマイクロRNA発現プロファイル解析からの悪性化因子の同定 (2011)
  • Author(s): 濱田晋, 佐藤賢一, 下瀬川徹
  • Organizer: JDDW 2011
  • Place of Presentation: 福岡
  • Year and Date: 2011-10-23
• [Presentation] 膵癌細胞株におけるEMT誘導因子MSX2の標的miRNAの検討 (2011)
  • Author(s): 濱田晋, 佐藤賢一, 廣田衛久, 菅野敦, 正宗淳, 菊田和宏, 粂潔, 海野純, 下瀬川徹
  • Organizer: 第42回膵学会
  • Place of Presentation: 弘前
  • Year and Date: 2011-07-29
• [Presentation] 膵癌の浸潤傾向を規定するmicroRNAの同定とその標的遺伝子の検討 (2011)
  • Author(s): 濱田晋, 佐藤賢一, 下瀬川徹
  • Organizer: 第97回消化器病学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-13
• [Presentation] THE TRANSCRIPTIONAL REGULATION OF CANCER STEM CELL-RELATED GENE ABCG2 BY THE HOMEOBOX GENE MSX2 (2011)
  • Author(s): Hamada S, Satoh K, Hirota M, Kanno A, Masamune A, Kikuta K, Kume K, Unno J, Shimosegawa T
  • Organizer: International Association of Pancreatology & Indian Pancreas Club 2011
  • Place of Presentation: Cochin, India
  • Year and Date: 2011-02-13
• [Presentation] Jagged1 contributes to the epithelial-mesenchymal transition as a target gene of TGF beta and BMP signals in pancreatic cancer cell line. (2010)
  • Author(s): S.Hamada, K.Satoh, M.Hirota, A.Kanno, H.Ito, T.Shimosegawa.
  • Organizer: Joint Meeting of the IAP and JPS 2010
  • Place of Presentation: 福岡
  • Year and Date: 2010-07-12