Regulation of hepatitis C virus secretion by the Hrs-dependent exosomal pathway
Project/Area Number |
22790630
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Gastroenterology
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Research Institution | Miyagi Cancer Center Research Institute |
Principal Investigator |
TAMAI Keiichi 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 主任研究員 (40509262)
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | ESCRT / Hrs / exosomes / HCV |
Research Abstract |
We demonstrated that Hrs, an ESCRT-0 component, is critical for the budding of HCV through the exosomal secretion pathway. Hrs depletion caused reduced exosome production, which paralleled with the decrease of HCV replication in the host cell, and that in the culture supernatant. Furthermore, both the core protein and an envelope protein of HCV were detected in the intraluminal vesicles of MVBs. These results suggested that HCV secretion from host cells requires Hrs-dependent exosomal pathway in which the viral assembly is also involved.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Regulation of hepatitis C virus secretion by the Hrs-dependent exosomal pathway2012
Author(s)
Tamai K, Shiina M, Tanaka N, Nakano T, Yamamoto A, Kondo Y, Kakazu E, Inoue J, Fukushima K, Sano K, Ueno Y, Shimosegawa T, Sugamura K.
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Journal Title
Virology
Volume: 22(2)
Issue: 2
Pages: 377-85
DOI
Related Report
Peer Reviewed
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[Journal Article] Powered, The AAA-ATPase VPS4 Regulates Extracellular Secretion and Lysosomal Targeting ofα-Synuclein2011
Author(s)
Takafumi Hasegawa, M. Konno, T. Baba, N. Sugeno, A. Kikuchi, M. Kobayashi, E. Miura, N. Tanaka, K. Tamai, K. Furukawa, H. Arai, F. Mori, K. Wakabayashi, M. Aoki, Y. Itoyama, A. Takeda
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Journal Title
Related Report
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[Journal Article] Lymphotropic HCV strain can infect human primary naive CD4+ cells and affect their proliferation and IFN-γ secretion activity2011
Author(s)
Kondo Y, Ueno Y, Kakazu E, Kobayashi K, Shiina M, Tamai K, Machida K, Inoue J, Wakui Y, Fukushima K, Obara N, Kimura O, Shimosegawa T
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Journal Title
J Gastroenterol
Volume: 46(2)
Issue: 2
Pages: 232-41
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Four-year study of lamivudine and adefovir combination therapy in lamivudine-resistant hepatitis B patients : influence of hepatitis B virus genotype and resistance mutation pattern2011
Author(s)
J. Inoue, Y. Ueno, Y. Wakui, H. Niitsuma, K. Fukushima, Y. Yamagiwa, M. Shiina, Y. Kondo, E. Kakazu, K. Tamai, N. Obara, T. Iwasaki, T. Shimosegawa
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Journal Title
J Viral Hepat
Volume: 18
Issue: 3
Pages: 206-215
DOI
Related Report
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[Journal Article] Exosome secretion of dendritic cells is regulated by Hrs, an ESCRT-0 protein2010
Author(s)
Tamai K, Tanaka N, Nakano T, Kakazu E, Kondo Y, Inoue J, Shiina M, Fukushima K, Hoshino T, Sano K, Ueno Y, Shimosegawa T, Sugamura K
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Journal Title
Biochem Biophys Res Commun
Volume: 399(3)
Issue: 3
Pages: 384-90
DOI
Related Report
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[Journal Article] Possible involvement and the mechanisms of excess trans-fatty acid consumption in severe NAFLD in mice2010
Author(s)
N. Obara, K. Fukushima, Y. Ueno, Y. Wakui, O. Kimura, K. Tamai, E. Kakazu, J. Inoue, Y. Kondo, N. Ogawa, K. Sato, T. Tsuduki, K. Ishida, T. Shimosegawa
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Journal Title
J Hepatol
Volume: 53
Issue: 2
Pages: 326-334
DOI
Related Report
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[Journal Article] Hepatitis B virus replication could enhance regulatory T cell activity by producing soluble heat shock protein 60 from hepatocytes2010
Author(s)
Kondo Y, Ueno Y, Kobayashi K, Kakazu E, Shiina M, Inoue J, Tamai K, Wakui Y, Tanaka Y, Ninomiya M, Obara N, Fukushima K, Ishii M, Kobayashi T, Niitsuma H, Kon S, Shimosegawa T
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Journal Title
J Infect Dis.
Volume: 202(2)
Issue: 2
Pages: 202-13
DOI
Related Report
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