Analysis on molecular mechanisms of proliferation and differentiation of hepatic stem/progenitor cells and development of animal model for treatment of liver diseases using hepatic stem cells
Project/Area Number |
22790632
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Gastroenterology
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
KAKINUMA Sei 東京医科歯科大学, 大学院・医歯学総合研究科, 寄附講座教員 (30372444)
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Project Period (FY) |
2010 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 肝臓学 / 再生医学 / 移植 / 細胞・組織 / 発生・分化 / 肝疾患治療 / 肝細胞移植 |
Research Abstract |
Investigators of this research project have analyzed on the molecular mechanisms of differentiation and proliferation of primary hepatic stem/progenitor cells purified by cell surface markers. We have also studied whether modulation of several molecules improves therapeutic efficacy of transplantation using hepatic stem/progenitor cells. We found that Wnt5a and Wnt5a-related molecules control the biliary differentiation of hepatic stem/progenitor cells. Next, our data indicated that loss of MMP2 or MMP14 worsens the efficacy of transplantation using hepatic stem/progenitor cells. These studies contribute to the progress of regenerative medical science and autologous stem cell therapy against liver diseases.
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Report
(3 results)
Research Products
(38 results)
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[Journal Article] Identification of novel N-(morpholine-4-carbonyloxy) amidine compounds as potent inhibitors against hepatitis C virus replication2012
Author(s)
Kusano-Kitazume A, Sakamoto N, Okuno Y, Sekine-Osajima Y, Nakagawa M, KakinumaS, Kiyonashi K, Nitta S, Murakawa M, AzumaS, Nishimura-Sakurai Y, Hagiwara M, Watanabe M
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Journal Title
Antimicrob Agent Chemother
Volume: 56(3)
Pages: 1315-23
Related Report
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[Journal Article] Data mining model using simple and readily available factors could identify patients at high risk for hepatocellular carcinoma in chronic hepatitis2012
Author(s)
Kurosaki M, Hiramatsu N, Sakamoto M, Suzuki Y, Iwasaki M, Tamori A, Matsuura K, Kakinuma S, Sakaguchi F, Sakamoto N, Nakagawa M, Izumi N.
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Journal Title
C.J Hepatol
Volume: 56(3)
Pages: 602-8
Related Report
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[Journal Article] Ochanomizu Liver Conference Study Group : Association of ITPA gene variant and serum ribavirin concentration with blood cells decline in pegylated interferon-alfa plus ribavirin therapy for chronic hepatitis C2012
Author(s)
Nakagawa M, Sakamoto N, Watanabe T, Nishimura-Sakurai Y, Onozuka I, Azuma S, Kakinuma S, Nitta S, Kiyohashi K, Kusano-Kitazume A, Murakawa M, Yoshino K, Itsui Y, Tanaka Y, Mizokami M, Watanabe M
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Journal Title
Related Report
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[Journal Article] Age and total ribavirin dose is an independent predictor of relapse among early virological responders to peg-interferon plus ribavirin therapy in chronic hepatitis C revealed by data mining analysis2012
Author(s)
Kurosaki M, Hiramatsu N, Sakamoto M, Suzuki Y, Iwasaki M, Tamori A, Matsuura K, Kakinuma S, Sugauchi F, Sakamoto N, Nakagawa M, Yatsuhashi H, Izumi N.
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Journal Title
Antivir Ther
Volume: 17
Pages: 35-43
Related Report
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[Journal Article] Cholestatic liver fibrosis and toxin-induced fibrosis are exacerbated in matrix metalloproteinase-2 deficient mice2011
Author(s)
Kakinuma S, Onozuka I, Kamiya A, MiyoshiM, Sakamoto N, Kiyohashi K, Watanabe T, Funaoka Y, Ueyama M, Nakagawa M, Koshikawa N, Seiki M, Nakauchi H, and Watanabe M.
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Journal Title
Biochem Biophys Res Commun
Volume: 406
Pages: 134-140
Related Report
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[Journal Article] Analysis of interferon signaling by infectious hepatitis C virus clones with substitutions of core amino acids 70 and 912011
Author(s)
Funaoka Y, Sakamoto N, Suda G, Itsui Y, Nakagawa M, Kakinuma S, Watanabe T, Nitta S, Kitazume A, Kiyohashi K, Murakawa M, Azuma S, Tsuchiya K, Watanabe M.
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Journal Title
J Virol
Volume: 85
Pages: 5986-94
Related Report
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[Journal Article] Pretreatment prediction of anemia progression by pegylated interferon a2b plus ribavirin combination therapy in chronic hepatitis C infection : Decision-tree analysis2011
Author(s)
Hiramatsu N, Kurosaki M, Sakamoto N, Iwasaki M, Sakamoto M, Suzuki Y, Sakaguchi F, Tamori A, Kakinuma S, Matsuura K, IzumiN.
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Journal Title
J Gastroenterol
Volume: 46
Pages: 1111-19
Related Report
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[Journal Article] Inhibitory effect of triterpenoid compound, with or without interferon-alpha, on hepatitis C virus infection2011
Author(s)
Watanabe T, Sakamoto N, Nakagawa M, Kakinuma S, Itsui Y, Nishimura-Sakurai Y, Ueyama M, Funaoka Y, Kitazume A, Nitta S, Kiyohashi K, Murakawa M, Azuma S, Tsuchiya K, Oooka S, Watanabe M.
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Journal Title
Antimicrob Agent Chemother
Volume: 55
Pages: 2537-45
Related Report
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[Journal Article] Conference Study Group. Serum interleukin-6 levels can predict resistance to treatment of chronic hepatitis C infection with pegylated-interferon alpha 2b plus ribavirin2011
Author(s)
Ueyama M, Nakagawa M, Sakamoto N, Onozuka I, Funaoka Y, Watanabe T, Nitta S, Kiyohashi K, Kitazume A, Murakawa M, Nishimura-Sakurai Y, Sekine-Osajima Y, Itsui Y, Azuma S, Kakinuma S, Watanabe M and the Ochanomizu-Liver
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Journal Title
Antivir Ther
Volume: 16
Pages: 1081-1091
Related Report
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[Journal Article] Studies on virus kinetics using infectious fluorescence-tagged hepatitis C virus cell culture2011
Author(s)
Yamamoto M, Sakamoto N, Nakamura T, Itsui Y, Nakagawa M, Nishimura-Sakurai Y, Kakinuma S, Azuma S, Kato T, Wakita T, Watanabe M.
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Journal Title
Hepatol Res
Volume: 41
Pages: 258-269
Related Report
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[Journal Article] Association of IL28B polymorphism with response to pegylated-interferon alpha plus ribavirin combination therapy in patients with chronic genotype 2 hepatitis C2011
Author(s)
Sakamoto N, Nakagawa M, Tanaka Y, Sekine-Osajima Y, Ueyama M, Kurosaki M, Nishida N, Tamori A, Nishimura-Sakurai Y, Itsui Y, Azuma S, Kakinuma S, Hige S, Ito Y, Tanaka E, Hiasa Y, Izumi N, Tokunaga K, Mizokami M, Watanabe M
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Journal Title
JMedical Virol
Volume: 83
Pages: 871-878
Related Report
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[Journal Article] Lnk/Sh2b3 regulates integrin aIIbb3 outside-in signaling in platelets leading to stabilization of developing thrombus in vivo2010
Author(s)
Takizawa H, Nishimura S, Takayama N, OdaA, Nishikii H, Morita Y, Kakinuma S, Yamazaki S, Okamura S, Tamura N, Goto S, Sawaguchi A, Manabe I, Takatsu K, NakauchiH, Takaki S, Eto K.
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Journal Title
J Clin Inve
Volume: 120
Pages: 179-190
Related Report
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[Journal Article] Cell culture and in-vivo analyses of plaque-derived cytopathic hepatitis C virus mutants2010
Author(s)
Mishima K, Sakamoto N, Sekine-Osajima Y, Nakagawa M, Itsui Y, Azuma S, Kakinuma S, Tuchiya K, Imamura M, Chayama K, Wakita T, Watanabe M.
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Journal Title
Virology
Volume: 405
Pages: 361-369
Related Report
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[Journal Article] IL-6-mediated intersubgenotypic variation of interferon sensitivity in hepatitis C virus genotype 2a/2b chimeric clones2010
Author(s)
Suda G, Sakamoto N, Itsui Y, Nakagawa M, Mishima K, Onuki-Karakama Y, Yamamoto M, Funaoka Y, Watanabe T, Kiyohashi K, Nitta S, Azuma S, Kakinuma S, Tuchiya K, ImamuraM, Hiraga N, Chayama K, Watanabe M.
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Journal Title
Virology
Volume: 407
Pages: 80-90
Related Report
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[Journal Article] Inhibition of HCV replication by a specific inhibitor of serin-arginine-rich protein kinase2010
Author(s)
Karakama Y, Sakamoto N, Itsui Y, Nakagawa M, Tasaka-Fujita M, Nishimura-Sakurai Y, Kakinuma S, Oooka S, Azuma S, Tsuchiya K, Onogi H, Hagiwara M, Watanabe M.
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Journal Title
Antimicrob Agent Chemother
Volume: 54
Pages: 3179-86
Related Report
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[Journal Article] ITPA gene variant protects against anemia induced by pegylated interferon-alfa and ribavirintherapy for Japanese patients with chronic hepatitis C2010
Author(s)
Sakamoto N, Tanaka Y, Nakagawa M, Yatsuhashi H, Nishiguchi S, Enomoto N, Azuma S, Nishimura-Sakurai Y, Kakinuma S, Nishida N, Tokunaga K, Honda M, Ito K, Mizokami M, Watanabe M.
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Journal Title
Hepatol Res
Volume: 40
Pages: 1063-1071
Related Report
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[Journal Article] Comparison of HCV-associated geneexpression and cell signaling pathways in cells with or without HCV replicon and in replicon-cured cells2010
Author(s)
Nishimura-Sakurai Y, Sakamoto N, MogushiK, Nagaie S, Nakagawa M, Itsui Y, Sekine-Osajima Y, Tasaka-Fujita M, Onuki-Karakama Y, Suda G, Mishima M, Yamamoto M, Ueyama M, Funaoka Y, Watanabe T, Chen CH, Kakinuma S, TsuchiyaK, Tanaka H, Enomoto N, Watanabe M.
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Journal Title
J Gastroentorol
Volume: 45
Pages: 523-36
NAID
Related Report
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[Journal Article] Mutations in the interferon sensitivity determining region and virological response to combination therapy with pegylated-interferon alpha 2b plus ribavirin in patients with chronic hepatitis C-1b infection2010
Author(s)
Nakagawa M, Sakamoto N, Ueyama M, Mogushi K, Nagaie S, Itsui Y, Azuma S, Kakinuma S, Tanaka H, Enomoto N, Watanabe M.
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Journal Title
J Gastroentorol
Volume: 45
Pages: 656-665
NAID
Related Report
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