| Project/Area Number |
22790649
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|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | The University of Tokushima |
|---|
Principal Investigator |
KUWANO Yuki 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (00563454)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Keywords | 選択的スプライシング / 大腸がん / Transformer 2β / SRタンパク質 / tra2 beta / 遺伝子発現 / 消化管上皮細胞 / マイクロアレイ / transformer 2 beta |
|---|
| Research Abstract |
In response to oxidative stress, colon cancer cells(HCT116) increased levels of TRA2β1 mRNA without changing its mRNA stability. TRA2βpromoters and Chip assay demonstrated that an Ets1-binding site was crucial for basal transcription, while three heat shock elements mediated the oxidant-induced transactivation of TRA2β. Tra2βknockdown caused apoptosis of HCT116 cells. Tra2βwere preferentially expressed in proliferative compartment of normal human colonic glands and adenocarcinomas, where Ets1 and HSF1 were also highly expressed.
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