The establishment of a screening system for low molecular compounds forβcell inducing activity.

• Project/Area Number: 22790653
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Kumamoto University
• Principal Investigator: SAKANO Daisuke 熊本大学, 発生医学研究所, COEリサーチ・アソシエイト (40571039)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ES細胞 / 膵臓 / β細胞 / インスリン / 糖尿病 / 低分子化合物 / スクリーニング / 発生・分化 / 再生医学 / 細胞・組織

Research Abstract

Establishment of an efficient method to induce pancreaticβ-cells from ES cells is an important research in order to obtain the knowledge for the treatment of human diabetes in the future. We screened more than 1, 300 small chemical compounds to increase the efficiency ofβ-cell induction. We found 10 hit compounds.
Based on the information of screening results, we found that a certain cell signaling pathway regulates the differentiation ofβ-cell and its insulin secreting ability.

Research Products

• [Presentation] Low molecular compounds screening system forβcell inducing activity (2011)
  • Author(s): Sakano D., Shiraki N., Kataoka M., Kume K. and Kume S.
  • Organizer: The workshop "Liver and Pancreas : From Development to Disease"
  • Place of Presentation: Baeza, Spain
• [Presentation] The establishment of a screening system for low molecular compounds forβcell inducing activity (2011)
  • Author(s): Sakano D., Shiraki N., Kataoka M., Kume K. and Kume S.
  • Organizer: International Society for Stem Cell Resaerch 9^<th> Annual Meeting
  • Place of Presentation: Tronto, Canada
• [Presentation] Low molecular compounds screening system for β cell inducing activity (2011)
  • Author(s): Sakano D., Shiraki N., Kataoka M., Kume K., Kume S.
  • Organizer: The workshop "Liver and Pancreas : From Development to Disease"
  • Place of Presentation: Baeza, Spain
• [Presentation] The establishment of a screening system for low molecular compounds for β cell inducing activity (2011)
  • Author(s): Sakano D., Shiraki N., Kataoka M., Kume K., Kume S.
  • Organizer: International Society for Stem Cell Research 9^<th> Annual Meeting
  • Place of Presentation: Tronto, Canada
• [Presentation] The establishment of a screening system for low moleculaz compounds for β cell inducing activity (2010)
  • Author(s): 坂野大介
  • Organizer: 分子生物学会 年会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2010-12-09
• [Presentation] The establishment of a screening system for low molecular compoumds for β cell inducing activity (2010)
  • Author(s): 坂野大介
  • Organizer: 2010 Global COE-IMEG Joint Summer Retreat Seminar
  • Place of Presentation: ホテルグリーンピア南阿蘇(熊本)
  • Year and Date: 2010-09-09
• [Presentation] The establishment of a screening system for low molecular compounds forβcell inducing activity (2010)
  • Author(s): Sakano, D., Shiraki, N., Kataoka, M., Kume, K and Kume, S.
  • Organizer: The 33rd Annual Meeting of Molecular Biology Society of Japan, The 83rd Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: 神戸国際会議場
• [Book] 「iPS細胞による糖尿病医療研究のこれから」-The view of stem cell therapy for type I diabetes-糖尿病 (2011)
  • Author(s): 坂野大介、白木伸明、粂昭苑
  • Publisher: 今川企画
• [Book] 糖尿病「iPS細胞による糖尿病医療研究のこれから」-The view of stem cell therapy for type I diabetes- (2011)
  • Author(s): 坂野大介、白木伸明、粂昭苑
  • Total Pages: 3
  • Publisher: 日本糖尿病学会
• [Patent(Industrial Property Rights)] 幹細胞からインスリン産生細胞への分化誘導を促進する低分子化合物および該化合物を用いた幹細胞からインスリン産生細胞への分化誘導方法 (2010)
  • Inventor(s): 粂昭苑、坂野大介、白木伸明、梅田香穂子、山添太士、粂和彦、上杉志成
  • Industrial Property Rights Holder: 国立大学法人熊本大学、国立大学法人京都大学
  • Filing Date: 2010-05-02