Cancer therapy by vector co-expressing p53 and miRNA with RNA interference

• Project/Area Number: 22790654
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Sapporo Medical University
• Principal Investigator: IDOGAWA Masashi 札幌医科大学, 医学部, 助教 (00404749)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: p53 / miRNA / MDM2 / p21 / アデノウイルスベクター / 癌 / アポトーシス / 遺伝子治療 / 治療抵抗性

Research Abstract

We constructed adenovirus vector which express artificial miRNA targeting MDM2. Infection with this vector and vector which co-express p53 and artificial miRNA targeting p21 enhanced apoptosis more efficiently in cancer cells. This result suggests that adenovirus-mediated transduction of p53 and miRNAs targeting anti-apoptotic p53 target genes is useful for therapy of human cancers.

Research Products

• [Journal Article] CHFR regulates the mitotic checkpoint by targeting PARP-1 for ubiquitination and degradation (2012)
  • Author(s): Kashima, L., Idogawa, M., Mita, H., Shitashige, M., Yamada, T., Ogi, K., Suzuki, H., Toyota, M., Ariga, H., Sasaki, Y., Tokino, T.
  • Journal Title: J. Biol. Chem Volume: 287 Issue: 16 Pages: 12975-12984
  • DOI: 10.1074/jbc.m111.321828
  • Peer Reviewed
• [Journal Article] p53 negatively regulates the hepatoma growth factor HDGF (2011)
  • Author(s): Sasaki Y, Negishi H, Idogawa M, Yokota I, Koyama R, Kusano M, Suzuki H, Fujita M, Maruyama R, Toyota M, Saito T, Tokino T
  • Journal Title: Cancer Res Volume: 71 Issue: 22 Pages: 7038-47
  • DOI: 10.1158/0008-5472.can-11-1053
  • Peer Reviewed
• [Journal Article] Identification and characterization of early growth response 2, a zinc-finger transcription factor, as a p53-regulated proapoptotic gene (2010)
  • Author(s): Yokota I, Sasaki Y, Kashima L, Idogawa M, Tokino T
  • Journal Title: International Journal of Oncology Volume: 37(6) Issue: 6 Pages: 1407-16
  • DOI: 10.3892/ijo_00000792
  • Peer Reviewed
• [Journal Article] Identification and characterization of early growth response 2, a zinc-finger transcription factor, as a p53-regulated proapoptotic gene. (2010)
  • Author(s): Yokota, et al.
  • Journal Title: International Journal of Oncology Volume: 37 Pages: 1407-16
  • Peer Reviewed
• [Presentation] ゲノム網羅的shRNAライブラリースクリーニングに基づく人工miRNA/p53共発現による癌治療 (2012)
  • Author(s): 井戸川雅史
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-10-03
• [Presentation] Apoptosis induction in cancer cells by the simultaneous transduction of p53 and artificial miRNAs based on the screening of genome-wide shRNA library (2012)
  • Author(s): Masashi Idogawa
  • Organizer: AACR 102st annual meeting
  • Place of Presentation: Orland, FL
  • Year and Date: 2012-04-06
• [Presentation] ゲノム網羅的shRNAライブラリースクリーニングに基づく人工miRNA/p53共発現による癌治療 (2011)
  • Author(s): 井戸川雅史, 佐々木泰史, 鹿島理沙, 篠村恭久, 今井浩三, 時野隆至
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-10-03
• [Presentation] Apoptosis induction in cancer cells by the simultaneous transduction of p53 and artificial miRNAs based on the screening of genome-wide shRNA library (2011)
  • Author(s): Idogawa M, Sasaki Y, Kashima L, Shinomura Y, Imai K, Tokino T
  • Organizer: American Association of Cancer Research(AACR) annual meeting
  • Place of Presentation: Orlando, FL
  • Year and Date: 2011-04-06
• [Presentation] p53とアポトーシス阻害的標的に対するmiRNAの単一アデノウイルス発現による癌治療 (2010)
  • Author(s): 井戸川雅史, 佐々木泰史, 鹿島理沙, 篠村恭久, 今井浩三, 時野隆至
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-23
• [Presentation] p53とアポトーシス阻害的標的に対するmiRNAの単一アデノウイルス発現による癌治療 (2010)
  • Author(s): 井戸川雅史
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-23
• [Presentation] Single adenovirus-mediated simultaneous expression of p53 and the artificial microRNAs targeting anti-apoptotic p53 target genes (2010)
  • Author(s): Idogawa M, Sasaki Y, Kashima L, Shinomura Y, Imai K, Tokino T
  • Organizer: American Association of Cancer Research(AACR) annual meeting
  • Place of Presentation: Washington D. C.
  • Year and Date: 2010-04-18
• [Presentation] Single adenovirus-mediated simultaneous expression of p53 and the artificial microRNAs targeting anti-apoptotic p53 target genes (2010)
  • Author(s): Masashi Idogawa
  • Organizer: AACR 101st annual meeting
  • Place of Presentation: Washington, D.C.
  • Year and Date: 2010-04-18