Diagnostic systems of diffuse type gastric cancer using by epigenomic approach

• Project/Area Number: 22790655
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Sapporo Medical University
• Principal Investigator: YAMAMOTO Eiichiro 札幌医科大学, 医学部, 助教 (60567915)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 癌 / メチル化 / マイクロアレイ / 遺伝子診断 / 癌予防 / 胃癌 / リスク予測

Research Abstract

Using methylated-CpG island amplification coupled with CpG island microarray(MCAM) analysis, RASGRF1 methylation was significantly elevated in gastric mucosafrom patients with either intestinal or diffuse type gastric cancer (GC). Our datasuggest that RASGRF1 methylation could be a useful biomarker with which to identifyindividuals at high risk for GC.We evaluated the clinical utility of DNA methylation as a biomarker ofmetachronous GC risk by performing a prospective study. Our results suggest thatmethylation of miR-34b/c in background gastric mucosae may be a useful marker topredict metachronous GC risk.

Research Products

• [Journal Article] Association between genomic alterations and metastatic behavior of colorectal cancer identified by array-based comparative genomic hybridization. (2013)
  • Author(s): Sawada T, Yamamoto E, Suzuki H
  • Journal Title: Genes Chromosomes Cancer Volume: 52(2) Issue: 2 Pages: 140-9
  • DOI: 10.1002/gcc.22013
  • Peer Reviewed
• [Journal Article] Aberrant Methylation of RASGRF1 Is Associated with an Epigenetic Field Defect and Increased Risk of Gastric Cancer. (2012)
  • Author(s): Takamaru H, Yamamoto E, Suzuki H.
  • Journal Title: Cancer Prev Res (Phila) Volume: 5(10) Issue: 10 Pages: 2012-2012
  • DOI: 10.1158/1940-6207.capr-12-0056
  • Peer Reviewed
• [Journal Article] Developing technologies for epigenomic analysis and clinical application of molecular diagnosis (2012)
  • Author(s): Eiichiro Yamamoto
  • Journal Title: Rinsho Byori Volume: 60 Pages: 637-43
  • NAID: 10030990656
• [Journal Article] DNA methylation and microRNA dysregulation in cancer. (2012)
  • Author(s): Suzuki Hiromu
  • Journal Title: Mol Oncol Volume: 6 Issue: 6 Pages: 567-78
  • DOI: 10.1016/j.molonc.2012.07.007
  • Peer Reviewed
• [Journal Article] Molecular Dissection of Premalignant Colorectal Lesions Reveals Early Onset of the CpG Island Methylator Phenotype. (2012)
  • Author(s): Yamamoto E, Suzuki H, Yamano HO
  • Journal Title: Am J Pathol Volume: 181(5) Issue: 5 Pages: 1847-61
  • DOI: 10.1016/j.ajpath.2012.08.007
  • Peer Reviewed
• [Journal Article] A novel pit pattern identifies the precursor of colorectal cancer derived from sessile serrated adenoma. (2012)
  • Author(s): Kimura T, Yamamoto E, Yamano HO.
  • Journal Title: Am J Gastroenterol. Volume: 107(3) Issue: 3 Pages: 460-9
  • DOI: 10.1038/ajg.2011.457
  • Peer Reviewed
• [Journal Article] Upregulation of miR-196a and HOTAIR drive malignant character in gastrointestinal stromal tumors (2012)
  • Author(s): Takeshi Niinuma
  • Journal Title: Cancer research Volume: 72 Issue: 5 Pages: 1126-36
  • DOI: 10.1158/0008-5472.can-11-1803
  • Peer Reviewed
• [Journal Article] Epigenetic alteration of DNA in mucosal wash fluid predicts invasiveness of colorectal tumors (2011)
  • Author(s): Seiko Kamimae
  • Journal Title: Cancer prevention research Volume: 4 Issue: 5 Pages: 674-83
  • DOI: 10.1158/1940-6207.capr-10-0214
  • Peer Reviewed
• [Journal Article] Genome-wide profiling of chromatin signatures reveals epigenetic regulation of microRNA genes in colorectal cancer (2011)
  • Author(s): Suzuki H
  • Journal Title: Cancer Res Volume: 71 Issue: 17 Pages: 5646-5658
  • DOI: 10.1158/0008-5472.can-11-1076
  • Peer Reviewed
• [Journal Article] Role of DNA Methylation in the Development of Diffuse-Type Gastric Cancer (2011)
  • Author(s): Eiichiro Yamamoto
  • Journal Title: Digestion Volume: 83 Pages: 241-249
  • Peer Reviewed
• [Journal Article] Methylation-associated silencing of microRNA-34b/c in gastric cancer and its involvement in an epigenetic field defect (2010)
  • Author(s): Hiromu Suzuki
  • Journal Title: Carcinogenesis Volume: 31 Pages: 2066-2073
  • Peer Reviewed
• [Presentation] Aberrant methylation of microRNA-34b/c as a biomarker for metachronous gastric cancer risk (2012)
  • Author(s): Eiichiro Yamamoto
  • Organizer: a prospective study 20th United European Gastroenterology(UEG) Week
  • Place of Presentation: アムステルダム
• [Presentation] DNA メチル化を指標とした胃発癌リスク予測 (2012)
  • Author(s): 山本英一郎
  • Organizer: 日本適応学会
  • Place of Presentation: 東京
• [Presentation] Aberrant methylation of microRNA-34b/c as a biomarker for metachronous gastric cancer risk: a prospective study (2012)
  • Author(s): Eiichiro Yamamoto
  • Organizer: 20th United European Gastroenterology (UEG) Week 2012
  • Place of Presentation: アムステルダム
• [Presentation] DNAメチル化を指標とした胃発癌リスク予測 (2012)
  • Author(s): 山本英一郎
  • Organizer: 日本適応学会
  • Place of Presentation: 東京
• [Presentation] エピゲノム解析法の発展と臨床応用 (2011)
  • Author(s): 山本英一郎
  • Organizer: 臨床検査学会
  • Place of Presentation: 岡山(招待講演)
  • Year and Date: 2011-11-18
• [Presentation] 大腸腫瘍におけるジェネティック・エピジェネティックな異常と臨床応用 (2011)
  • Author(s): 山本英一郎
  • Organizer: 電気泳動学会
  • Place of Presentation: 横浜(招待講演)
  • Year and Date: 2011-11-12
• [Presentation] DNAメチル化・エピゲノム (2011)
  • Author(s): 山本英一郎
  • Organizer: 病理学会
  • Place of Presentation: 横浜(招待講演)
  • Year and Date: 2011-04-28
• [Presentation] エピゲノム解析法の発展と臨床応用 (2011)
  • Author(s): 山本英一郎
  • Organizer: 臨床検査学会
  • Place of Presentation: 岡山
• [Presentation] 大腸腫瘍におけるジェネティック・エピジェネティックな異常と臨床応用 (2011)
  • Author(s): 山本英一郎
  • Organizer: 電気泳動学会
  • Place of Presentation: 横浜
• [Presentation] DNAメチル化・エピゲノム (2011)
  • Author(s): 山本英一郎
  • Organizer: 日本病理学会
  • Place of Presentation: 横浜
• [Presentation] Epigenetic silencing of microRNAs in human gastric cancer (2010)
  • Author(s): Eiichiro Yamamoto
  • Organizer: 18^<th> United European Gastroenterology Week
  • Place of Presentation: Barcelona
  • Year and Date: 2010-10-25
• [Presentation] エピゲノム的アプローチからみた未分化型胃癌発生の分子機構 (2010)
  • Author(s): 山本英一郎
  • Organizer: JDDW 2010
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-10-13
• [Presentation] エピゲノム的アプローチからみた未分化型胃癌発生の分子機構 (2010)
  • Author(s): 山本英一郎
  • Organizer: JDDW
  • Place of Presentation: 横浜
• [Presentation] Epigenetic silencing ofmicroRNAs in human gastric cancer (2010)
  • Author(s): Eiichiro Yamamoto
  • Organizer: 18thUnited European Gastroenterology Week
  • Place of Presentation: Barcelona
• [Patent(Industrial Property Rights)] RASGRF1 の定量的メチル化測定を用いた発癌リスク予測 (2010)
  • Inventor(s): 豊田実、山本英一郎、篠村恭久、山野泰穂
  • Industrial Property Rights Holder: 札幌医科大学
  • Filing Date: 2010-10-26
• [Patent(Industrial Property Rights)] RASGRF1の定量的メチル化測定を用いた発癌リスク予測 (2010)
  • Inventor(s): 山本英一郎
  • Industrial Property Rights Holder: 札幌医科大学
  • Industrial Property Number: 2010-239932
  • Filing Date: 2010-10-26