|Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
We investigated whether TLR9 agonist CpG DNA protects mice from colonic inflammation through activated plasmacytoid dedritic cells(pDCs) and B cell proliferation. Administration of CpG DNA significantly suppressed colonic inflammation of DSS-induced colitis, and we could detect production of regulatory cytokines in mesenteric lymph nodes. Although we could not detect regulatory B cells as well as its induction by co-cultured with CpG DNA stimulated pDC. These results suggest that B cells, especially regulatory B cells didn' t concerned to protective effect of CpG DNA to mouse DSS colitis.