| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
Investigation of hepatitis B virus (HBV) has been hampered by the absence of accessible in vitro modeling tools. The aim of this study is to design an in vitro model of HBV infection that would support long-term replication. Immortalized normal human hepatocytes, or primary human hepatocytes isolated from chimeric mice with human liver were cultured with three-dimensional (3D) culture system to obtain a tissue-like formation and exposed to HBV-infected serum samples. The HBV-DNA level in supernatants of the culture was higher than 10^5 copies/ml and continued to be detectable for >1 month. The extracellular HBsAg was detected during the culture period. The 3D culture system with hepatocytes provides an accessible in vitro tool to investigate HBV cell entry, replication and susceptibility to new anti-viral agents for multiple HBV genotypes/subgenotypes.
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