Application of control method to inhibit pancreatic cancer cell growth and metastasis by the regulation of lumican glycosylation.
| Project/Area Number |
22790675
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ルミカン / 膵臓癌 / 細胞増殖 / 細胞浸潤 / MMP-9 / プロテオグリカン / 糖鎖解析 |
|---|
| Research Abstract |
Lumican expression in the stromal tissues of pancreatic ductal adenocarcinoma(PDAC) correlates with tumor invasion, and tends to correlate with poor prognosis. We used gene transfection techniques to examine the biological roles of lumican secreted from PDAC cells. Lumican-transfected PDAC cells secreted a 70-kDa lumican protein and had an active ERK pathway. Transfection stimulated PDAC cell growth, increased cell adhesion to laminin, inhibited cell invasion, and decreased active matrix metalloproteinase-9. Down-regulation of lumican using siRNA resulted in opposite cell behavior. Thus, the 70-kDa lumican secreted by PDAC cells plays important roles in cell growth and invasion.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Comparison of fixation methods for preservation of morphology, RNAs, and proteins from paraffin-embedded human cancer cell-implanted mouse models2011
Author(s)
Matsuda Y, Fujii T, Suzuki T, Yamahatsu K, Kawahara K, Teduka K, Kawamoto Y, Yamamoto T, Ishiwata T, Naito Z
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Journal Title
J Histochem Cytochem
Volume: 59
Issue: 1
Pages: 68-75
DOI
Related Report
Peer Reviewed
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[Journal Article] Comparison of fixation methods for preservation of morphology, RNAs, and proteins from paraffin-embedded human cancer cell-implanted mouse models.2011
Author(s)
Matsuda Y, Fujii T, Suzuki T, Yamahatsu K, Kawahara K, Teduka K, Kawamoto Y, Yamamoto T, Ishiwata T, Naito Z.
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Journal Title
J Histochem Cytochem.
Volume: 59
Pages: 68-75
Related Report
Peer Reviewed
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[Presentation] Lumican, a member of small leucine-rich proteoglycan family, as a new target to develop molecularly-targeted drug for pancreatic cancer2010
Author(s)
T.Yamamoto, T.Ishiwata, Y.Matsuda, M.Onda, K.Yamahatsu, K.Kawahara, Y.Kawamoto, E.Uchida, Z.Naito
Organizer
American association for cancer research, 101th annual meeting 2010
Place of Presentation
米国・ワシントン
Year and Date
2010-04-19
Related Report
