Investigation the mechanisms of chronicity from acute hepatitis B using a next generation sequencer

• Project/Area Number: 22790679
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Research Institute, International Medical Center of Japan
• Principal Investigator: ITO Kiyoaki 独立行政法人国立国際医療研究センター (50551420)
• Co-Investigator(Renkei-kenkyūsha): SUGIYAMA Masasya (20612427)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: B型急性肝炎 / 慢性化 / SNPs / HBV / 次世代シーケンサー / GWAS / HLA-DP / ジェノタイプ

Research Abstract

We have investigated the both host and viral factors associated with chronicity from acute hepatitis B. Rs3077 and rs9277535 were genotyped by the invader method which we have developed. However, these SNPs were not associated with chronicity from acute hepatitis B in adults. We have analyzed the viral factor using a next generation sequencer, and could obtain the specific findings to analyze the number of clones.

Research Products

• [Journal Article] Impairment of hepatitis B virus virion secretion by single-amino-acid substitutions in the small envelope protein and rescue by a novel glycosylation site (2010)
  • Author(s): Ito K, Qin Y, Guarnieri M, Garcia T, Kwei K, Mizokami M, Zhang J, Li J, Wands JR, Tong S
  • Journal Title: Journal of Virology Volume: 84(24) Pages: 12850-61
  • Peer Reviewed
• [Journal Article] Japan National Hospital Acute Hepatitis Study Group. Dynamic epidemiology of acute viral hepatitis in Japan (2010)
  • Author(s): Yano k, Tamada Y, Yatsuhashi H, Komori A, Abiru S, Ito K, Masaki N, Mizokami M, Ishibashi H
  • Journal Title: Intervirology Volume: 53(1) Pages: 70-5
  • Peer Reviewed
• [Journal Article] Dynamic epidemiology of acute viral hepatitis in Japan (2010)
  • Author(s): Yano k, Tamada Y, Yatsuhashi H, Kotnori A, Abiru S, Ito K, Masaki N, Mizokami M, Ishibashi H; Japan National Hospital Acute Hepatitis Study Group
  • Journal Title: Intervirology Volume: 53(1) Issue: 1 Pages: 70-5
  • DOI: 10.1159/000252788
  • Peer Reviewed
• [Journal Article] Impairment of hepatitis B virus virion secretion by single-amino-acid substitutions in the small envelope protein and rescue by a novel glycosylation site. (2010)
  • Author(s): Ito K, Qin Y, Guarnieri M, Garcia T, Kwei K, Mizokami M, Zhang J, Li J, Wands JR, Tong S.
  • Journal Title: Journal of Virology Volume: 84(24) Pages: 12850-61
  • Peer Reviewed
• [Journal Article] Dynamic epidemiology of acute viral hepatitis in Japan. (2010)
  • Author(s): Yano k, Tamada Y, Yatsuhashi H, Komori A, Abiru S, Ito K, Masaki N, Mizokami M, Ishibashi H ; Japan National Hospital Acute Hepatitis Study Group.
  • Journal Title: Intervirology. Volume: 53(1) Pages: 70-5
  • Peer Reviewed
• [Presentation] Risk factors for long-term persistence of serum HBsAg following acute hepatitis B virus infection in Japan (2012)
  • Author(s): Ito K, et al
  • Organizer: The 22^<nd> Conference of the Asian Pacific Association for the Study of the Liver
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2012-02-17
• [Presentation] B型急性肝炎ジェノタイプAに関する全国調査(中間集計) (2011)
  • Author(s): 伊藤清顕、正木尚彦、溝上雅史
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京
• [Book] 肝胆膵B型肝炎に影響を与える個体差 (2011)
  • Author(s): 伊藤清顕
• [Book] 臨床消化器内科B型肝炎ウイルス再活性化 (2011)
  • Author(s): 伊藤清顕、正木尚彦
• [Book] 肝胆膵B型肝炎に影響を与える個体差 (2011)
  • Author(s): 伊藤清顕
  • Total Pages: 6
  • Publisher: アークメディア
• [Book] 臨床消化器内科B型肝炎ウイルス再活性化 (2011)
  • Author(s): 伊藤清顕、正木尚彦
  • Total Pages: 5
  • Publisher: 日本メディカルセンター
• [Book] 肝胆膵 B型肝炎に影響を与える個体差 (2011)
  • Author(s): 伊藤清顕
  • Total Pages: 6
  • Publisher: アークメディア
• [Book] 臨床消化器内科 B型肝炎ウイルス再活性化 (2011)
  • Author(s): 伊藤清顕、正木尚彦
  • Total Pages: 5
  • Publisher: 日本メディカルセンター
• [Book] 総合臨床感染症制御のための公衆衛生の役割:ウイルス性肝炎 (2010)
  • Author(s): 伊藤清顕、溝上雅史
• [Book] 綜合臨床感染症制御のための公衆衛生の役割:ウイルス性肝炎 (2010)
  • Author(s): 伊藤清顕、溝上雅史
  • Total Pages: 6
  • Publisher: 永井書店
• [Book] 綜合臨床 感染症制御のための公衆衛生の役割:ウイルス性肝炎 (2010)
  • Author(s): 伊藤清顕、溝上雅史
  • Total Pages: 6
  • Publisher: 永井書店