Gene therapy for myocardial infarction by using lentivirus encoding kit ligand

Research Project

Project/Area Number	22790717
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Circulatory organs internal medicine
Research Institution	Kagoshima University
Principal Investigator	HIGUCHI Koji 鹿児島大学, 医歯学総合研究科, 特任助教 (90448580)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000) Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	分子心臓病態学 / 心筋梗塞 / 遺伝子治療
Research Abstract	The purpose of this study is to evaluate the effect of kit ligand(KL) isoform against myocardial infarction(MI). The KL expression in transduced cells was confirmed by western blotting and flow cytometry. Endogenous KL-1, KL-2 and c-kit expression in infarcted mouse hearts were evaluated. We found that KL-2 expression in mouse hearts was diminished after MI. Therefor gene therapy using lentivirus encoding KL-2 may improve the prognosis after MI. We were supposed to finish in vivo experiments in this year, however we have difficulties in making large scale virus. We will continue to do in vivo experiments.