Asssociation of monocyte subsets with vulnerablity characteristics of coronary plaque

• Project/Area Number: 22790723
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Wakayama Medical University
• Principal Investigator: KASHIWAGI Manabu 和歌山県立医科大学, 医学部, 学内助教 (70453181)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 臨床心血管病態学 / 単球 / 不安定プラーク / 冠動脈CT / サロゲートマーカー

Research Abstract

Monocytes play an active role in coronary artery disease. Monocytes in human peripheral blood are heterogeneous. Differential expression of CD14 and CD16 enables monocytes to be divided into two subsets : CD14+CD16. monocytes and CD14+CD16+monocytes, called as "inflammatory" and "pro-inflammatory". We examined the relation between monocyte subsets and vulnerability characteristics of coronary plaques by using multidetector computed tomography. In our results, the relative proportion of CD14+CD16+monocytes was positively correlated with remodeling index and negatively correlated with CT attenuation value. Furthermore, we investigated the involvement of Toll-like receptor 4(TLR4) expression on two monocyte subsets in the pathologic processes related to acute coronary syndrome. The expression levels of TLR4 on CD14+CD16+monocytes were significantly elevated in patients with AMI. Moreover, the up-regulation of TLR4 on admission was remarkably decreased at the chronic phase after AMI. TLR overexpression on CD14+CD16+monocytes in AMI might be associated with the pathogenesis of AMI. In conclusion, CD14+CD16+monocytes show a potential for a surrogate marker of vulnerable plaques and acute coronary syndrome.

Research Products

• [Journal Article] Differential expression of Toll-like receptor 4 and human monocyte subsets in acute myocardial infarction (2012)
  • Author(s): Kashiwagi M, Imanishi T, Ozaki Y, Satogami K, Masuno T, Wada T, NakataniY, Ishibashi K, Komukai K, Tanimoto T, Ino Y, Kitabata H, Akasaka T
  • Journal Title: Atherosclerosis Volume: 221 Issue: 1 Pages: 249-53
  • DOI: 10.1016/j.atherosclerosis.2011.12.030
  • Peer Reviewed
• [Journal Article] Association of monocyte subsets with vulnerability characteristics of coronary plaques as assessed by 64-slice multidetector computed tomography in patients with stable angina pectoris (2010)
  • Author(s): Kashiwagi M, Imanishi T, Tsujioka H, Ikejima H, Kuroi A, Ozaki Y, Ishibashi K, Komukai K, Tanimoto T, Ino Y, Kitabata H, Hirata K, Akasaka T
  • Journal Title: Atherosclerosis Volume: 212 Issue: 1 Pages: 171-6
  • DOI: 10.1016/j.atherosclerosis.2010.05.004
• [Journal Article] Association of monocyte subsets with vulnerability characteristics of coronary plaques assessed by 64-slice multidetector computed tomography in patients with stable angina pectoris (2010)
  • Author(s): Manabu Kashiwagi
  • Journal Title: Atherosclerosis Volume: 212 Pages: 171-176
  • Peer Reviewed
• [Presentation] ヒト末梢血単球と冠動脈CTを用いて評価した冠動脈プラークの不安定性の関連性の検討 (2010)
  • Author(s): 柏木学
  • Organizer: 第58回日本心臓病学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-17
• [Presentation] ヒト末梢血単球と冠動脈CTを用いて評価した冠動脈プラークの不安定性の関連性の検討 (2010)
  • Author(s): 柏木学
  • Organizer: 第58回 心臓病学会 総会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2010-09-17