Epigenomic analysis of DNA methylation pattern in cardiomyocyte development and evaluation of stem cell differentiation methods.

• Project/Area Number: 22790730
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Keio University
• Principal Investigator: ODA Mayumi 慶應義塾大学, 医学部, 助教 (80567511)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: DNAメチル化 / エピゲノム / 細胞特異的遺伝子発現 / 心筋細胞 / 転写調節 / 細胞種特異的遺伝子発現 / エピジェネティクス / 細胞分化 / ゲノムワイド / 次世代シークエンサー / 幹細胞 / バイオインフォマティクス

Research Abstract

Epigenome is thought to represent the developmental potential of given cells. However, it has been unknown where is the genomic regions that define the ability of cells, like stemness or spectrum of cell-type specific potentials. In this study, we sought

Research Products

• [Journal Article] Genetic analysis of essential cardiac transcription factors in 256 patients with non-syndromic congenital heart defects. (2012)
  • Author(s): Kodo K, Nishizawa T, Furutani M, Arai S, Ishihara K, Oda M, Makino S, Fukuda K, Takahashi T, Matsuoka R, Nakanishi T, Yamagishi H.
  • Journal Title: Circulation Journal Volume: 76 Pages: 1703-1711
  • NAID: 10030503588
  • Peer Reviewed
• [Journal Article] Late-replicating heterochromatin is characterized by decreased cytosine methylation in the human genome. (2011)
  • Author(s): Suzuki M, Oda M, Ramos MP, Pascual M, Lau K, Stasiek E, Agyiri F, Thompson RF, Glass JL, Jing Q, Sandstrom R, Fazzari MJ, Hansen RS, Stamatoyannopoulos JA, McLellan AS, Greally JM
  • Journal Title: Genome Research Volume: 21 Pages: 1833-1840
  • Peer Reviewed
• [Journal Article] Genetic analysis of essential cardiac transcription factors in 256 patients with non-syndromic congenital heart defects. (2011)
  • Author(s): Kodo K, Nishizawa T, Furutani M, Arai S, Ishihara K, Oda M, Makino S, Fukuda K, Takahashi T, Matsuoka R, Nakanishi T, Yamagishi H
  • Journal Title: Circulation Journal Volume: 76 Pages: 1703-1711
  • NAID: 10030503588
  • Peer Reviewed
• [Presentation] Cell Type-specific Gene Body DNA Hypomethylationin Contributes to Transcriptional Efficiency in Cardiomyocyte Maturation. (2012)
  • Author(s): Mayumi Oda, Shinji Makino, Hirokazu Enomoto, Kojiro Yae, Ruri Kaneda, Shinsuke Yuasa and Keiichi Fukuda
  • Organizer: 日本エピジ ェネティクス研究会年会
  • Place of Presentation: 一橋学術総合センター(東京)
• [Presentation] Escape of Gene Body DNA Methylation in Cardiac Genes is Cooperative with the Cell Type-specific Expression Patterns. (2012)
  • Author(s): Mayumi Oda, Shinji Makino, Hirokazu Enomoto, Ruri Kaneda, Shinsuke Yuasa and Keiichi Fukuda
  • Organizer: Weinstein meeting 2012
  • Place of Presentation: シカゴ(アメリ カ)
• [Presentation] Cell Type-specific Gene Body DNA Hypomethylationin Cardiac Genes Contributes to Transcriptional Efficiency in CardiomyocyteMaturation (2012)
  • Author(s): Mayumi Oda, Shinji Makino, Hirokazu Enomoto, Kojiro Yae, Ruri Kaneda, Shinsuke Yuasa, and Keiichi Fukuda.
  • Organizer: 日本循環器学会年会
  • Place of Presentation: 福岡国際会議場（福岡）
• [Presentation] Escape of Gene Body DNA Methylation in Cardiac Genes is Cooperative with the Cell Type-specific Expression Patterns (2012)
  • Author(s): Mayumi Oda, Shinji Makino, Hirokazu Enomoto, Ruri Kaneda, Shinsuke Yuasa and Keiichi Fukuda.
  • Organizer: Weinstein meeting 2012
  • Place of Presentation: シカゴ（アメリカ）
• [Presentation] Cell Type-specific Gene Body DNA Hypomethylationin Contributes to Transcriptional Efficiency in Cardiomyocyte Maturation (2012)
  • Author(s): Mayumi Oda, Shinji Makino, Hirokazu Enomoto, Kojiro Yae, Ruri Kaneda, Shinsuke Yuasa and Keiichi Fukuda.
  • Organizer: 日本エピジェネティクス研究会年会
  • Place of Presentation: 一橋学術総合センター（東京）
• [Presentation] Cell Type-specific Gene Body DNA Hypomethylationin Cardiac Genes Contributes to Transcriptional Efficiency in Cardiomyocyte Maturation. (2011)
  • Author(s): Mayumi Oda, Shinji Makino, Hirokazu Enomoto, Kojiro Yae, Ruri Kaneda, Shinsuke Yuasa, and Keiichi Fukuda
  • Organizer: 日本循環器学会年会
  • Place of Presentation: 福岡国際 会議場(福岡)
• [Presentation] DNA Methylation Changes in The b-MHC Locus Mediate Gene Regulation by Antisense RNA in Postnatal Cardiomyocyte Development. (2011)
  • Author(s): Mayumi Oda, Shinji Makino, Shinsuke Yuasa, Sung Han Yoon, Yuta Higashikuse, Kojiro Yae, Ruri Kaneda, Mitsushige Murata, Motoaki Sano, John M. Greally and Keiichi Fukuda
  • Organizer: American Heart Association, Scientific Sessions
  • Place of Presentation: オーランド(アメリカ)
• [Presentation] Gene Body DNA Methylation Level in Cardiac Myosin Heavy Chain (MHC) Gene Locus is Cell Type- and Locus- Specifically Suppressed and Contributes to the Transcriptional Efficacy at Postnatal Development of Mouse Cardiomyocytes. (2011)
  • Author(s): Mayumi Oda, Shinji Makino, Shinsuke Yuasa, Ruri Kaneda, Masako Suzuki, John M. Greally and Keiichi Fukuda
  • Organizer: Weinstein meeting 2011
  • Place of Presentation: シンシナティ(アメリ カ)
• [Presentation] Gene Body DNA Methylation Level in Cardiac Myosin Heavy Chain (MHC) Gene Locus is Cell Type- and Locus- Specifically Suppressed and Contributes to the Transcriptional Efficacy at Postnatal Development of Mouse Cardiomyocytes. (2011)
  • Author(s): Mayumi Oda, Shinji Makino, Shinsuke Yuasa, Ruri Kaneda, Masako Suzuki, John M. Greally and Keiichi Fukuda.
  • Organizer: Weinstein meeting 2011
  • Place of Presentation: シンシナティ（アメリカ）
• [Presentation] DNA Methylation Changes in The b-MHC Locus Mediate Gene Regulation by Antisense RNA in Postnatal Cardiomyocyte Development (2011)
  • Author(s): Mayumi Oda, Shinji Makino, Shinsuke Yuasa, Sung Han Yoon, Yuta Higashikuse, Kojiro Yae, Ruri Kaneda, Mitsushige Murata, Motoaki Sano, John M. Greally and Keiichi Fukuda
  • Organizer: American Heart Association, Scientific Sessions
  • Place of Presentation: オーランド（アメリカ）
• [Presentation] Cell Type-specific Gene Body DNA Hypomethylation in Cardiac Myosin Heavy Chain (MHC) Gene Locus Contributes to Efficient Transcription at Postnatal Development of Mouse Cardiomyocytes. (2011)
  • Author(s): Mayumi Oda
  • Organizer: CDB Symposium 2011 "Epigenetic Landscape in Development and Disease"
  • Place of Presentation: RIKEN CDB, Kobe, Hyogo JAPAN.
• [Presentation] DNA Methylation Changes in the b-MHC Locus Mediate Gene Regulation by Antisense RNA in Mouse Postnatal Cardiomyocyte Development (2010)
  • Author(s): Mayumi Oda
  • Organizer: American Heart Association Scientific Session 2010
  • Place of Presentation: McCormick Place, Chicago, IL, USA.
  • Year and Date: 2010-11-17
• [Remarks] Research map
  • URL: http://researchmap.jp/odamame/
• [Remarks] 坂口光洋記念講座ホームページ:小田真由美
  • URL: http://www.careerpath-prj.keio.ac.jp/sakaguchi/scholar/m_oda/
• [Remarks] Research map
  • URL: http://researchmap.jp/odamame/
• [Remarks] 坂口光洋記念講座ホームページ：小田真由美
  • URL: http://www.careerpath-prj.keio.ac.jp/sakaguchi/scholar/m_oda/
• [Remarks]
  • URL: http://researchmap.jp/odamame/