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分子イメージングを利用した冠動脈硬化性病変の非侵襲的診断法の開発

Research Project

Project/Area Number 22790741
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

TAKAHAMA Hiroyuki  独立行政法人国立循環器病研究センター, 心臓血管内科, 医師 (10570301)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords循環器 / 分子イメージング / 循環器・高血圧 / スプライシン
Research Abstract

Molecular imaging using bubble immune-liposomes and echocardiography to detect thrombus in left atrial appendage in canine hearts.
Background : Although the presence of thrombus in left atrial appendage was widely known to increase the risk of cerebral infarction in patients with atrial fibrillation, but its morphology was amorphous and has been difficult for the detection and histological evaluation using the conventional echocardiography in the clinical settings. Liposomes are nanoparticles that can deliver various agents to target tissues such as bubble and could be coated with antibody or peptide binding to the target molecule. We investigated whether immune-bubble liposomes could detect the thrombus in left atrial appendage in dogs.
Methods and Results : Bubble liposomes coated with RGD peptide(Arg-Gly-Asp) were prepared by reversephase evaporation vesicle(REV) and sonication methods. RGD peptide has an affinity to integrin(αvβ3) which was abundant in the thrombus. We ligated the left atrial appendage of anesthetic dogs for 120 minutes(n=4). After the identification of thrombus evaluated by echocardiography, firstly we intravenously administed saline under the observation of echocardiography as control study(Figure A). After 10 minutes of the infusion of saline, we intravenously administed immune-bubble lipomes into the dogs. Thrombus in left auricular appendage was clearly identified using echocardiography(Figure B.) Left atrial appendage samples were obtained at the end of the experimental protocol(Figure C.) and its histological analysis revealed to be a thrombus.
Conclusion : By adding immune-bubble liposomes, thrombus in left atrial appendage can be clearly recognized. Echocardiography using immune-bubble liposomes provide the diagnostic information of the thrombus in left atrial appendage in dogs. Bubble liposomes targeting to the molecule could be a novel echocardiographic modality.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (3 results)

All 2012 2011 2010

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] A histamine H(2) receptor blocker ameliorates development of heart failure in dogs independently of β-adrenergic receptor blockade.2010

    • Author(s)
      Takahama H, et al.
    • Journal Title

      Basic Res Cardiol.

      Volume: 105 Pages: 787-794

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Molecular imaging using bubble immune-liposomes and echocardiography to detect thrombus in left atrial appendage in canine hearts2012

    • Author(s)
      Takahama H, et al
    • Organizer
      2012年アメリカ心臓病学会の学術集会に上記演題を投稿中である
    • Related Report
      2011 Final Research Report
  • [Presentation] Heart rate reduction during heart failure hospitalization is powerful predictor of cardiac events in patients with acute decompensated heart failure regardless heart rate at discharge2011

    • Author(s)
      高濱博幸
    • Organizer
      ヨーロッパ心臓病学会
    • Place of Presentation
      パリ(フランス)
    • Year and Date
      2011-08-31
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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