Exploration of micro RNA regulating lung fibrosis and epithelial mesenchymal transition
| Project/Area Number |
22790763
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 肺線維症 / マイクロRNA / 分子標的治療 |
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| Research Abstract |
To determine the role of epithelial Pten in lung fibrosis, we compared the responses to bleomycin administration between wild-type and bronchioalveolar epithelium-specifically Pten-deleted[SP-C-rtTA/(tetO)_7-Cre/Pten^Δ/Δ(SOPten^Δ/Δ) mouse. Epithelial-derived myofibroblasts were increased in epithelium-specific Pten-deficient mice. Lungs of bleomycin-treated SOPten^Δ/Δmice showed increased pAkt, pS6K, Snai expressions. Akt inactivation definitively saved SOPtenΔ/Δmice. Finally, we detected a reduction of PTEN expression and AKT hyperactivation in the AECs of human IPF lungs. Our results highlight epithelial Pten as a crucial gatekeeper controlling lung fibrosis by modulating integrity of AECs.
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Report
(3 results)
Research Products
(10 results)
