The role of autophagy in COPD pathogenesis

• Project/Area Number: 22790771
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Jikei University School of Medicine
• Principal Investigator: HARA Hiromichi 東京慈恵会医科大学, 医学部, 助教 (70398791)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 慢性閉塞性肺疾患 / オートファジー / クレアチンキナーゼB(CKB) / CKB

Research Abstract

We demonstrated the inhibitory role of autophagy in bronchial epithelial cell senescence induced by cigarette smoke extract(CSE) exposure. Although creatine kinase B(CKB) was not involved in regulation of autophagy function, decrease of CKB expression enhanced CSE-induced cellular senescence. Furthermore, CKB expression levels were reduced in bronchial epithelial cells isolated from COPD patients compared to those from non-smokers. Taken together, reduced CKB expression levels in lung may be involved in COPD pathogenesis through the acceleration of cellular senescence.

Research Products

• [Journal Article] (2012)
  • Author(s): Hara H, Araya J, Takasaka N, Fujii S, Kojima J, Yumino Y, Shimizu K, Ishikawa T, Numata T, Kawaishi M, Saito K, Hirano J, Odaka M, Morikawa T, Hano H, Nakayama K, Kuwano K
  • Journal Title: Am J Respir Cell Mol Biol Volume: 46(3) Pages: 306-312
  • Peer Reviewed
• [Journal Article] Involvement of creatin kinase B in cigarette smoke-induced bronchial epithelial cell senescence (2012)
  • Author(s): Hiromichi Hara
  • Journal Title: Am J Respir Cell Mol Biol Volume: 46(3) Pages: 306-12
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): 原弘道, 藤井さと子, 荒屋潤, 皆川俊介, 弓野陽子, 小島淳, 沼田尊功, 河石真, 中山勝敏, 桑野和善
  • Journal Title: 分子呼吸器病学 Volume: Vol.15, No.1 Pages: 94-96
• [Journal Article]
  • Author(s): Fujii S, Hara H, Araya J, Takasaka N, Kojima J, Ito S, Minagawa S, Yumino Y, Ishikawa T, Numata T, Kawaishi M, Hirano J, Odaka M, Morikawa T, Nishimura S L, Nakayama K, Kuwano K
  • Journal Title: Oncoimmunology Volume: (in press)
  • Peer Reviewed
• [Presentation] Cigarette smoke-induced oxidative modification of CKB is involved in the pathogenesis of COPD in terms of acceleration of bronchial epithelial cell senescence (2011)
  • Author(s): H Hara, J Araya, N Takasaka, J Kojima, Y Yumino, S Fujii, T Numata, M Kawaishi, J Hirano, M Odaka, T Morikawa, K Nakayama, and K Kuwano
  • Organizer: European Respiratory Society Annual congress
  • Place of Presentation: Amsterdam
  • Year and Date: 2011-09-28
• [Presentation] COPDにおける細胞老化とクレアチニンキナーゼに関する検討 (2011)
  • Author(s): 原弘道、荒屋潤、高坂直樹、弓野陽子、藤井さと子、小島淳、石川威夫、沼田尊功、河石真、中山勝敏、桑野和善
  • Organizer: 日本呼吸器学学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-04-22
• [Presentation] The Involvement of Autophagy in Cigarette Smoking Extract-induced Cellular Senescence in Human Bronchial Epithelial Cells American (2010)
  • Author(s): H Hara, S Nojiri, J Araya, Y Yumino, S Minagawa, J Kojima, T Numata, M Kawaishi, M Odaka, K Nakayama, T Morikawa, K Kuwano
  • Organizer: Thoracic Society International Conference
  • Place of Presentation: New Orleans
  • Year and Date: 2010-05-18