Regulatory analysis of DNA methylation in pulmonary fibrosis

• Project/Area Number: 22790772
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Kinki University
• Principal Investigator: PARK Ah-mee 近畿大学, 医学部, 講師 (70469245)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 肺線維化 / メチル化 / 筋線維芽細胞 / メチル化食 / 線維化 / DNMT3B / シリカ / DNAメチル化 / 転写因子 / プロモーター活性

Research Abstract

The object of this study is to clarify the epigenetic changes in pulmonary fibrosis. In vivo study using silica-induced mouse fibrosis model revealed that the DNA methylation level was unchanged, but the protein level of DNMT3B, which catalysis DNA methylation, was increased in fibrosis lung. In vitro study also showed the increase of this protein. The increase of DNMT3B protein may play important role in pulmonary fibrosis.

Research Products

• [Journal Article] p38MAPK suppresses chronic pancreatitis by regulating HSP27 and BAD expression (2012)
  • Author(s): Park AM, Kudo M, Hagiwara S, Tabuchi M, Watanabe T, Munakata H, Sakurai T.
  • Journal Title: Free Radic Biol Med Volume: 52(11-12) Issue: 11-12 Pages: 2284-91
  • DOI: 10.1016/j.freeradbiomed.2012.03.010
  • Peer Reviewed
• [Journal Article] Munakata H, Sakurai T. Activation of c-Jun N-terminal kinase in non-cancerous liver tissue predicts a high risk of recurrence after hepatic resection for hepatocellular carcinoma (2012)
  • Author(s): Hagiwara S, Kudo M, Chung H, Ueshima K, Inoue T, Haji S, Watanabe T, Park AM,
  • Journal Title: Hepatol Res. Volume: 42(4) Issue: 4 Pages: 394-400
  • DOI: 10.1111/j.1872-034x.2011.00932.x
  • Peer Reviewed
• [Journal Article] Conditioned media from lung cancer cell line A549 and PC9 inactivate pulmonary fibroblasts by regulating protein phosphorylation. (2012)
  • Author(s): Park AM, Hayakawa S, Honda E, Mine Y, Yoshida K, Munakata H.
  • Journal Title: Arch Biochem Biophys. Volume: 15;518(2) Issue: 2 Pages: 133-41
  • DOI: 10.1016/j.abb.2011.12.012
  • Peer Reviewed
• [Journal Article] Involvement of angiotensin II and reactive oxygen species in pancreatic fibrosis. (2011)
  • Author(s): Sakurai T, Kudo M, Fukuta N, Nakatani T, Kimura M, Park AM,, Munakata H.
  • Journal Title: Pancreatology Volume: 11 Suppl 2 Issue: 2 Pages: 7-13
  • DOI: 10.1159/000323478
  • Peer Reviewed
• [Journal Article] Mechanism of anthracycline-mediated down-regulation of GATA4 in the heart. (2011)
  • Author(s): Park AM, Nagase H, Liu L, Vinod Kumar S, Szwergold N, Wong CM, Suzuki YJ.
  • Journal Title: Cardiovasc Res. Volume: 1;90(1) Issue: 1 Pages: 97-104
  • DOI: 10.1093/cvr/cvq361
  • Peer Reviewed
• [Journal Article] The cancer stem cell marker CD133 is a predictor of the effectiveness of S1+ pegylated interferon-2b therapy against advanced hepatocellular carcinoma. (2011)
  • Author(s): Hagiwara S, Kudo M, Ueshima K, Chung H, Yamaguchi M, Takita M, Haji S, Kimura M, Arao T, Nishio K, Park AM, Munakata H.
  • Journal Title: J Gastroenterol. Volume: 46(2) Issue: 2 Pages: 212-21
  • DOI: 10.1007/s00535-010-0294-5
  • Peer Reviewed
• [Journal Article] The cancer stem cell marker CD133 is a predictor of the effectiveness of S1+ pegylated interferon alpha-2b therapy against advanced hepatocellular carcinoma. (2011)
  • Author(s): Satoru Hagiwara
  • Journal Title: J Gastroenterol Volume: 46 Pages: 212-221
  • Peer Reviewed
• [Journal Article] Mechanism of anthracycline-mediated down-regulation of GATA4 in the heart. (2011)
  • Author(s): Ah-Mee Park
  • Journal Title: Cardiovasc Res. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Pulmonary hypertension-induced GATA4 activation in the right ventricle. (2010)
  • Author(s): Park AM, Wong CM, Jelinkova L, Liu L, Nagase H, Suzuki YJ.
  • Journal Title: Hypertension Volume: 56(6) Issue: 6 Pages: 1145-51
  • DOI: 10.1161/hypertensionaha.110.160515
  • Peer Reviewed
• [Journal Article] Pulmonary hypertension-induced gata4 activation in the right ventricle (2010)
  • Author(s): Ah-Mee Park
  • Journal Title: Hypertension Volume: 56 Pages: 1145-1151
  • Peer Reviewed
• [Presentation] Role of heat shock protein 27 in myofibroblast (2012)
  • Author(s): 朴雅美 本田映子 宗像浩
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] HSP27の筋線維芽細胞における役割 (2012)
  • Author(s): 朴雅美
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] The role of p38 MAPK in pancreatitis of male Wistar Bonn/Kobori rat (2011)
  • Author(s): 朴雅美 櫻井俊治 原田八千代 宗像 浩
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Hiroshi Munakata Role of heat shock protein 27 in pulmonary fibrosis and epithelial-mesenchymal transition of alveolar epithelial cells. (2011)
  • Author(s): Ah-Mee Park
  • Organizer: 第76回日本インターフェロン・サイトカイ ン学会、第19回マクロファージ分子細胞生 物学国際シンポジウム 合同開催学術集会
  • Place of Presentation: 大阪
• [Presentation] Role of heat shock protein 27 in pulmonary fibrosis and epithelial-mesenchymal transition of alveolar epithelial cells (2011)
  • Author(s): Ah-Mee Park
  • Organizer: 第76回日本インターフェロン・サイトカイン学会、第19回マクロファージ分子細胞生物学国際シンポジウム
  • Place of Presentation: 全日空ゲートタワーホテル大阪
• [Presentation] インテグリンα11とcalcium and integrin binding protein 1の相互作用 (2010)
  • Author(s): 吉田浩二
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] 肺線維化におけるHSP27の役割 (2010)
  • Author(s): 朴雅美
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] 肺線維化におけるHSP27の役割 (2010)
  • Author(s): Ah-Mee Park, Hiroshi Munakata
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸