Directed differentiation of human iPS cells into nephrogenic mesoderm towards regenerative medicine strategy for kidney diseases
| Project/Area Number |
22790786
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
OSAFUNE Kenji 京都大学, iPS細胞研究所, 准教授 (80502947)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | iPS細胞 / 中間中胚葉 / 腎臓 / 分化誘導 / 再生 / 人工多能性幹細胞 / 増殖因子 / ES細胞 / 胚性幹細胞 / レポーター細胞株 |
|---|
| Research Abstract |
We have succeeded in generating reporter human iPS cell lines for OSR1 gene, which is a specific marker for an embryonic germ layer, the intermediate mesoderm(IM) that gives rise to kidney. By using combinatorial treatment of growth factors, we have established highly efficient differentiation methods to induce human iPS cells into IM cells at an induction rate of more than 90%. We have also confirmed that these human IM cells can differentiate into cells constituting IM derivative organs, such as kidney, adrenal cortex and genitals.
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Report
(3 results)
Research Products
(48 results)
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[Presentation] 腎再生研究の進歩2011
Author(s)
長船健二
Organizer
第41回日本腎臓学会西部学術大会
Place of Presentation
あわぎんホール(徳島県)(招待講演)
Year and Date
2011-09-30
Related Report
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[Presentation] Induction and monitoring of intermediate mesoderm from human iPSCs and ESCs2011
Author(s)
Mae SI, Shono A, Shiota F, Yasuno T, Nishimura N, Arai S, Takahashi K, Matsubara A, Nakayama N, Ogawa S, McMahon AP, Yamanaka S, Osafune K
Organizer
9th Annual Meeting, International Society for Stem Cell Research
Place of Presentation
トロント(カナダ)
Year and Date
2011-06-16
Related Report
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[Presentation] iPS細胞2011
Author(s)
長船健二
Organizer
第54回日本腎臓学会学術総会
Place of Presentation
パシフィコ横浜(神奈川県)(招待講演)
Year and Date
2011-06-16
Related Report
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[Presentation] Induction and monitoring of intermediate mesoderm from human iPSCs and ESCs2011
Author(s)
Mae SI, Shono A, Shiota F, Yasuno T, Nishimura N, Arai S, Takahashi K, Matsubara A, Nakayama N, Ogawa S, McMahon AP, Yamanaka S, Osafune K.
Organizer
9th Annual Meeting, International Society for Stem Cell Research
Place of Presentation
Metro Toronto Convention Centre, Toronto, Ontario, Canada
Related Report
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