Development of EPO-producing cell using immortalize system
| Project/Area Number |
22790792
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 移植・再生医療 / 遺伝子 / エリスロポエチン / 腎性貧血 / 不死化細胞 |
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| Research Abstract |
We developed immortalized cells derived from several cell lines to establish immortalize erythropoietin-producing cells. Erythropoietin production was easily detected using the reporter line system. Erythropoietin-producing cells which expressed GFP were selected by cell sorter. In addition, we developed an erythropoietin-overexpress vector, and confirmed the long-term production of erythropoietin in vector-tranfected cells.
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Report
(3 results)
Research Products
(75 results)
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[Journal Article] Blockage of AT1 receptors protects the blood-brain barrier and improve cognition in Dahl salt-sensitive hypertensive rats.2011
Author(s)
Pelish N, Hosomi N, Ueno M, Nakano D, Hitomi H, Mogi M, Shimada K, Kobori H, Horiuchi M, Sakamoto H, Matsumoto M, Kohno M, Nishiyama A.
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Journal Title
Am J Hypertens
Volume: 24
Issue: 3
Pages: 362-368
DOI
Related Report
Peer Reviewed
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