| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
We hypothesis that the expression of laminin alpha1 is important for mouse brain ischemia and the regeneration. To prove the hypothesis, we made mouse ischemia model and immunostained the brain. Laminin alpha1 did not expression at 6 hour after ischemia and the mainly expression was started at 24 hour after ischemia in the basement membrane of neovessel. Moreover, the expression of laminin alpha1 was observed at 7 days after ischemia which was regeneration stage. Other hand, in the lesion of ischemia, a part of microgila expressed laminin alpha1. These results indicate that laminin alpha1 associates with newly form of brain vessels after brain injury. Next, to examine whether the expression of laminin alpha1 is important for brain injury after ischemia, we generated laminin alpha1 conditional knockout(Lama1 CKO) mouse. We observed the phenotype in Lama1 CKO mice. Lama1 CKO showed markedly behavioral abnormality and we clarified that the abnormality was caused by defect of cerebellum formation using immunohistochemistry, western blot, and slice culture method. On the other hand, the apparent abnormality was not detected in the cerebrum of Lama1 CKO mice compared with wild type mice. These results indicate that it is possible to carry out the operation of ischemia model to Lama1 CKO mice. Finally, as our result, laminin alpha1 associates with the angiogenesis and the accumulation of microglia in encephalopathy and regeneration after ischemia and it is suggested that laminin alpha1 is important for the brain tissue remodeling after ischemia.
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