Regulation of the apoE gene in astrocytes as a mechanism for recovery of brain injury

• Project/Area Number: 22790868
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Metabolomics
• Research Institution: Chubu University
• Principal Investigator: RO Ei 中部大学, 生物機能開発研究所, 研究員 (80381862)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: アポリポ蛋白質E / FGF-1 / プロモーター / アストロサイト / LXR / apoE / report gene / luciferase assay / Akt/PI3 / MEK/ERK / astrocyte

Research Abstract

We investigated the mechanism for FGF-1 to upregulate apoE transcription. FGF-1 was shown to enhance apoE transcription through the increase of interaction of the liver X receptor(LXR)α, a nuclear receptor for oxysterol being involved in sterol homeostasis, with a conserved direct repeat 4(DR4) sequence in LXR response element(LXRE) present in the apoE promoter. This reaction was mediated by the increase of LXRα expression and by the production of its ligand.