Regulatory mechanism of AMP kinase-fatty acid oxidation axis in the food preference center

• Project/Area Number: 22790875
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Metabolomics
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: OKAMOTO Shiki 生理学研究所, 発達生理学研究系, 助教 (40342919)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脳・神経 / ウィルス / メタボリックシンドローム / AMPキナーゼ

Research Abstract

Paraventricular hypothalamus(PVH) is implicated in the selection of high carbohydrate diet(HCD) and high fat diet(HFD). I clarified that AMP-activated protein kinase(AMPK) and fatty acid oxidation(FAO) in CRH neurons of PVH are critical for food selection whether HCD or HFD. Constitutively-active(CA)-AMPK in PVH neurons increases HCD intake but inhibits HFD intake dependent on FAO in PVH. Fasting increased AMPK activity in the PVH and induced high preference for carbohydrate to maintain the energy balance rapidly. Inhibition of CRH expression using shRNA technique could completely reverse this preference change. Subpopulation of CRH neurons in the anterior part of PVH increased intracellular Ca2+levels and might evoke via AMPK-FAO axis. These results reveal a novel role of AMPK-FAO pathway in CRH neurons of PVH in food selection behaviour under physiological conditions.

Research Products

• [Presentation] AMP-kinase in the paraventricular hypothalamus regulates food selection behavior in mice (2012)
  • Author(s): 岡本士毅、箕越靖彦
  • Organizer: 第89回日本生理学会大会シンポジウム48(入澤宏・彩記念JPS優秀論文賞(入澤賞)シンポジウム
  • Place of Presentation: 長野県、松本文化会館
  • Year and Date: 2012-03-31
• [Presentation] AMP-kinase in the paraventricular hypothalamus regulates food selection behavior in mice (2012)
  • Author(s): 岡本士毅
  • Organizer: 第89回日本生理学会大会シンポジウム48
  • Place of Presentation: 長野県、松本文化会館(入澤宏・彩記念JPS優秀論文賞(入澤賞)シンポジウム)
  • Year and Date: 2012-03-31
• [Presentation] 食餌嗜好性に及ぼす視床下部室傍核CRHニューロンのAMPキナーゼ調節作用 (2011)
  • Author(s): 岡本士毅、箕越靖彦
  • Organizer: 第32回日本肥満学会シンポジウム3
  • Place of Presentation: 兵庫県、淡路夢舞台国際会議場
  • Year and Date: 2011-09-23
• [Presentation] 食餌嗜好性に及ぼす視床下部室傍核CRHニューロンのAMPキナーゼ調節作用 (2011)
  • Author(s): 岡本士毅
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府、大阪府立大学
  • Year and Date: 2011-09-19
• [Presentation] 肥満マウスの食餌嗜好性に及ぼす視床下部室傍核AMPキナーゼの調節作用 (2010)
  • Author(s): 岡本士毅、箕越靖彦
  • Organizer: 第31回肥満学会シンポジウム2 :神経内シグナル異常と肥満
  • Place of Presentation: 群馬県、前橋テルサ
  • Year and Date: 2010-10-01
• [Presentation] 肥満マウスの食餌嗜好性に及ぼす視床下部室傍核AMPキナーゼの調節作用 (2010)
  • Author(s): 岡本士毅
  • Organizer: 第31回肥満学会 シンポジウム2:神経内シグナル異常と肥満
  • Place of Presentation: 群馬県、前橋テルサ
  • Year and Date: 2010-10-01
• [Presentation] Regulatory role of AMP-kinase in the paraventricular hypothalamus in calorie intake and food preference (2010)
  • Author(s): 岡本士毅、箕越靖彦
  • Organizer: 第11回国際肥満学会(International Cogress on Obessity : IOC2010)サテライトシンポジウム「Regulation of Feeding and Energy Metabolism by the Brain」
  • Place of Presentation: ストックホルム、Clarion Holel Sign(スウェーデン)
  • Year and Date: 2010-07-10
• [Presentation] Regulatory role of AMP-kinase in the paraventricular hypothalamus in calorie intake and food preference (2010)
  • Author(s): 岡本士毅
  • Organizer: 第11回国際肥満学会(International Congress on Obessity : IOC2010)サテライトシンポジウム「Regulation of Feeding and Energy Metabolism by the Brain」
  • Place of Presentation: ストックホルム、Clarion Holel Sign(スウェーデン)
  • Year and Date: 2010-07-10
• [Remarks]
  • URL: http://www.nips.ac.jp/endoclin/