Genome-wide analysis of acute myeloid leukemia using high-density oligonucleotide arrays

• Project/Area Number: 22790900
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: NAKAZAKI Kumi 東京大学, 保健・健康推進本部, 助教 (70550432)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 急性骨髄性白血病 / SNPアレイ / 遺伝子解析 / ゲノム / アレイ解析 / 癌

Research Abstract

Genome-wide analysis with single nucleotide polymorphisms(SNP) arrays was conducted for samples from 103 cases with acute myeloid leukemia(AML). We applied high-resolution oligonucleotide microarrays(GeneChip(R) 100K or 500K arrays) which enabled us to detect allele specific copy number alterations in tumors systemically and sensitively. As a result, Lots of chromosomal aberrations including very small regions were shown in this study, and uniparental disomy was detected in 29% of AML. Homozygous mutations were highly frequently in UPD regions.