Analysis for hematopoiesis and hematologic diseases by L3MBTL1 conditional knockout mice.

• Project/Area Number: 22790903
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: ARAI Satoko 東京大学, 大学院・医学系研究科, 講師 (60422276)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 造血 / クロマチン制御 / 血液疾患

Research Abstract

It has been supposed that dysfunction of L3MBTL1 may cause hematological diseases. In this study, we propose the construction of L3MBTL1-conditional knock out mouse in which L3MBTL1 can be deleted specifically in the hematopoietic cells to know the function of L3MBTL1 in hematopoiesis. Unfortunately, we could not accomplish generation of the mutant mice. In addition, I performed the pull-down assay by using Halotag-fusion L3MBTL1 protein expressed in human leukemic cell lines to clarify the L3MBTL1-conmplex in hematopoietic cells, and the candidates for the associated proteins were obtained.