B cell differentiation induced by miR-126
| Project/Area Number |
22790904
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Tokai University (2011)
The University of Tokyo (2010) |
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Principal Investigator |
KOTANI Ai 東海大学, 創造科学技術研究機構, 特任准教授 (00517477)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | miRNA / 細胞運命決定 / 急性リンパ性白血病 / 細胞運命 / IRS1 |
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| Research Abstract |
MicroRNAs(miRNAs) are small noncoding RNAs vital to many cell functions that act post-transcriptionally to decrease the expression of target mRNAs. In this study we investigated miRNA involvement in cell differentiation in acute leukemia. In ALLs with MLL rearrangement, the expression of miR-126 is downregulated compared to other types of ALLs. When we restored miR-126 expression in a cell line derived from an MLL-AF4 ALL patient(SEM), B cells markers CD20 and CD19 were significantly upregulated, suggesting that miR-126 had induced B cell differentiation in MLL-AF4 ALL. A cDNA array measuring gene expression also showed upregulation of B cell related genes in SEM cells overexpressing miR126.When we overexpressed miR-126 in normal hematopoietic cells in vivo via mouse bone marrow transplantation, we also observed an increase in the number of CD19+cells in the peripheral blood, revealing that miR-126 induces B cell differentiation both in leukemia and normal hematopoietic cells. The mechanism of B cell differentiation by miR-126 was investigated using the coculture system of mouse fetal liver cells and TSt-4 stromal cells, which showed that miR-126 induces differentiation in uncommitted cells but not in cells committed to a lymphoid or myeloid lineage.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] Imatinib mesylate directly impairs class switch recombination through downregulation of AID2012
Author(s)
Toyotaka Kawamata, Jun Lu, Tadayuki Sato, Masafumi Tanaka, Hitoshi Nagaoka, Yasutoshi Agata, Takae Toyoshima, Kazuaki Yokoyama, Naoki Oyaizu, Naoya Nakamura, Kiyoshi Ando, Arinobu Tojo, and Ai Kotani. equally contributed corresponding author
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Journal Title
Blood
Volume: 119(13)
Pages: 3123-7
Related Report
Peer Reviewed
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[Journal Article] Imatinib mesylate directly impairs class switch recombination through downregulation of AID.2012
Author(s)
Kawamata T, Lu J, Sato T, Tanaka M, Nagaoka H, Agata Y, Toyoshima T, Yokoyama K, Oyaizu N, Nakamura N, Ando K, Tojo A, Kotani A
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Journal Title
Blood
Volume: 119
Issue: 13
Pages: 3123-3127
DOI
Related Report
Peer Reviewed
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[Journal Article] miRNAs in normal and malignant B cells2010
Author(s)
Ai Kotani, Ratanakanit Harnprasopwat, Toyotaka Kawamata, Takae Toyoshima, Arinobu Tojo, correspondence author, equally contributed.
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Journal Title
International Journal of Hematology
Volume: 92
Pages: 255-61
NAID
Related Report
Peer Reviewed
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[Presentation] EBV positive secretary miRNAs2012
Author(s)
Jen Lu, Ai Kotani
Organizer
Keystone Symposia's 2012 Meeting on "Gene Silencing by Small RNAs"
Place of Presentation
Vancouver, British Columbia Fairmond Hotel
Year and Date
2012-02-07
Related Report
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