Functional analysis of autophagy in T cells of healthy donors and SLE patients

• Project/Area Number: 22790924
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Tohoku University
• Principal Investigator: FUJII Hiroshi 東北大学, 病院, 助教 (30531321)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 膠原病学 / オートファジー / T細胞 / 全身性エリテマトーデス / 免疫学 / 膠原病 / アポトーシス

Research Abstract

We successfully developed the high thorough-put and high sensitive method to measure autophagic activity of human activated T cells on flow cytometry. Using this method, we analysed the functional significance of autophagy for stress by cell replication, drugs. We confirmed that autophagy had inhibitory effects on apoptotic cell death of activated T cells.

Research Products

• [Presentation] 顕著な脳炎を呈した中枢神経ループス患者における新規抗EphB2自己抗体の同定 (2012)
  • Author(s): 白井剛志、藤井博司、中村恭平、渡部龍、田島結実、高澤徳彦、石井智徳、張替秀郎
  • Organizer: 第56回日本リウマチ学会総会
  • Place of Presentation: 東京
  • Year and Date: 2012-04-28
• [Presentation] フローサイトメトリーを用いたヒトプライマリT細胞におけるオートファジーの定量と機能解析 (2012)
  • Author(s): 渡部龍、藤井博司、白井剛志、田島結実、高澤徳彦、石井智徳、張替秀郎
  • Organizer: 日本分子生物学会第12回春季シンポジウム
  • Place of Presentation: 山梨
  • Year and Date: 2012-04-25
• [Presentation] Retroviral vector system identified FLRT2 as a novel cell surface autoantigen against anti-endothelial cell antibody in systemic lupus erythematosus (2011)
  • Author(s): 白井剛志、藤井博司, 他
  • Organizer: American college of rhumeatology
  • Place of Presentation: 米国、シカゴ
  • Year and Date: 2011-11-07
• [Presentation] Retroviral vector system identified FLRT2 as a novel cell surface autoantigen against anti-endothelial cell antibodies in systemic lupus erythematosus. (2011)
  • Author(s): Shirai. T , Fujii. H, Ono. M, Takasawa. N , Ishii. T, Harigae. H.
  • Organizer: 米国リウマチ学会(ACR)
  • Place of Presentation: シカゴ
  • Year and Date: 2011-11-05
• [Presentation] レトロウィルスベクターシステムを用いた抗血管内皮細胞抗体対応自己抗原FLRT2の同定 (2011)
  • Author(s): 白井剛志、藤井博司、中村恭平、渡部龍、田島結実、高澤徳彦、石井智徳、張替秀郎
  • Organizer: 第55回日本リウマチ学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2011-07-19
• [Presentation] Retroviral vector system identified FLRT2 as a novel cell surface autoantigen against anti-endothelial cell antibody in systemic lupus erythematosus (2011)
  • Author(s): 白井剛志、藤井博司, 他
  • Organizer: 日本リウマチ学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-07-19
• [Presentation] hTERT insufficiency in rheumatoid arthritis causes PUMA-mediated T-cell apoptosis (2010)
  • Author(s): 藤井博司
  • Organizer: 第14回国際免疫学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-25