Elucidation of the role of keratinocyte and inflammatory cells for development of atopic dermatitis

• Project/Area Number: 22790949
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: YASUDA Takuwa 独立行政法人理化学研究所, アレルギー免疫遺伝研究チーム, 研究員 (00373374)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: アトピー性皮膚炎 / 黄色ブドウ球菌 / 好塩基球 / 肥満細胞 / IgE / 好中球 / 好酸球

Research Abstract

In this study, using a newly generated mutant mouse(spade mouse), which spontaneous develops atopic dermatitis like skin disease, we investigated a mechanisms for development of dermatitis.
Our data showed that bacterial component from S. aureus and pruritic stimulus elicited by PAR2-agonist peptide had a potential for an activating factor affecting epidermal keratinocytes in the development of dermatitis. Infiltration of granulocytes, especially basophils were higher in spade mouse skin than in wild-type mouse skin at just before the appearance of dermatitis, and were increased in skin of spade mouse after the development of dermatitis. On the other hand, our results suggested that mast cells and IgE played a supportive role in the development of dermatitis in our mouse model.

Research Products

• [Journal Article] Development and Function of Invariant Natural Killer T cells Producing TH2-and TH17-cytokines (2012)
  • Author(s): Hiroshi Watarai, Etsuko Sekine-Kondo, Tomokuni Shigeura, Yasutaka Motomura, Takuwa Yasuda, Rumi Satoh, Hisahiro Yoshida, Masato Kubo, Hiroshi Kawamoto, Haruhiko Koseki, Masaru Taniguchi
  • Journal Title: PLoS Biology Volume: 10(2)
  • Peer Reviewed
• [Journal Article] Development and Function of Invariant Natural Killer T cells Producing TH2- and TH17-cytokines (2012)
  • Author(s): Watarai H, Sekine-Kondo E, Shigeura T, Motomura Y, Yasuda T, Satoh R, Yoshida H, Kubo M, Kawamoto H, Koseki H, Taniguchi M
  • Journal Title: PLoS Biol Volume: 10 Issue: 2 Pages: e1001255-e1001255
  • DOI: 10.1371/journal.pbio.1001255
  • Peer Reviewed
• [Presentation] Stepwise progressive atopic dermatitis induced by a signaling molecule hyperactivation (2010)
  • Author(s): 安田琢和
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-08-25
• [Presentation] Stepwise progressive atopic dermatitis induced by a signaling molecule hyperactivation, Laboratory for Immunogenetics, RIKEN Research Center for Allergy and Immunology (2010)
  • Author(s): Takuwa YASUDA, Hisahiro YOSHIDA
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe, Japan
• [Patent(Industrial Property Rights)] 皮膚炎症性疾患の診断方法 (2011)
  • Inventor(s): 吉田尚弘、安田琢和、若菜茂晴、久保允人
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Patent Publication Number: 2011-083279
  • Acquisition Date: 2011-04-28
  • Overseas
• [Patent(Industrial Property Rights)] 皮膚炎症性疾患の診断方法 (2010)
  • Inventor(s): 吉田尚弘、安田琢和、若菜茂晴、久保允人
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 2010-208532
  • Filing Date: 2010-09-16
  • Overseas