Analysis of the effects of TIM-1 on respiratory epithelial cells on bronchial asthma

• Project/Area Number: 22790973
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: Shinshu University
• Principal Investigator: KOBAYASHI Norimoto 信州大学, 医学部, 助教 (00362129)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: T cell Immunoglobulin mucin domain protein (TIM)-1 / 気管支喘息 / 気道上皮細胞 / TIM-1 / 肺胞上皮細胞 / アレルギー / IL-33 / TSLP / ファゴサイトーシス / 自然免疫

Research Abstract

To access the function of TIM-1 on respiratory epithelial cells, the human type II alveolar epithelium cell line A549 was used. Although A549 expressed high level of TIM-1 on the cell surface, it was not able to phagocyte apoptotic cells. Moreover, A549 has different TIM-1 mutations among each cell clone. These mutations probably affect the binding between TIM-1 and apoptotic cells.