• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Induction of hematopoietic stem/progenitor cells and functional blood cells from human ES/iPS cells

Research Project

Project/Area Number 22790979
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionKyoto University

Principal Investigator

NIWA Akira  京都大学, iPS細胞研究所, 特定研究員 (20546999)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords造血発生 / 中胚葉分化 / 無血清培養 / 再生医学 / ES/iPS細胞 / ヒトES/iPS細胞 / 造血細胞分化 / ヒトES細胞 / ヒトiPS細胞 / 造血分化 / 造血幹細胞
Research Abstract

Elucidating the in vitro differentiation of human embryonic stem(ES) and induced pluripotent stem(iPS) cells is important for understanding both normal and pathological hematopoietic development invivo. For this purpose, a robust and simple hematopoietic differentiation system that can faithfullytrace in vivo hematopoiesis is necessary. In this study, we established a novel serum-free monolayerculture that can trace the in vivo hematopoietic pathway from ES/iPS cells to functional definitiveblood cells via mesodermal progenitors. Stepwise tuning of exogenous cytokine cocktails induced thehematopoietic mesodermal progenitors via primitive streak cells. These progenitors were thendifferentiated into various cell lineages depending on the hematopoietic cytokines present. Moreover, single cell deposition assay revealed that common bipotential hemoangiogenic progenitors wereinduced in our culture. Our system provides a new, robust, and simple method for investigating themechanisms of mesodermal and hematopoietic differentiation.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (19 results)

All 2012 2011 2010 2009

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (11 results) Book (4 results)

  • [Journal Article] Induced pluripotent stem cells from CINCAsyndrome patients as a model for dissecting somatic mosaicism and drugdiscovery2012

    • Author(s)
      Tanaka T, Takahashi K, Yamane M, Tomida S, Nakamura S, Oshima K, Niwa A, Nishikomori R, Kambe N, Hara H, Mitsuyama M, Morone N, Heuser JE, Yamamoto T, Watanabe A, Sato-Otsubo A, Ozawa S, AsakaI, Heike T, Yamanaka S, Nakahata T, and Saito MK
    • Journal Title

      Blood

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotentcells via mesodermal progenitors2011

    • Author(s)
      Niwa A, Heike T, Umeda K, Oshima K, KatoI, Sakai H, Suemori H, Nakahata T, Saito MK
    • Journal Title

      PLoS One

      Volume: 6(7)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neutrophil differentiation from human-induced pluripotent stem cells2011

    • Author(s)
      Morishima T, Watanabe K, Niwa A, Fujino H, Matsubara H, Adachi S, Suemori H, Nakahata T, Heike T
    • Journal Title

      J Cell Physiol

      Volume: 226(5) Pages: 1283-91

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors2011

    • Author(s)
      Niwa A, Heike T, Umeda K, et al.
    • Journal Title

      PloS One

      Volume: 6 Issue: 7 Pages: e22261-e22261

    • DOI

      10.1371/journal.pone.0022261

    • NAID

      120003324603

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] ヒト多能性幹細胞由来造血支持細胞を用いた、二次元無血清条件における造血分化能の定量的評価の試み2011

    • Author(s)
      丹羽明
    • Organizer
      第10回日本再生医療学会
    • Place of Presentation
      東京
    • Year and Date
      2011-03-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト多能性幹細胞由来造血支持細胞を用いた、二次元無血清条件における造血分化能の定量的評価の試み2011

    • Author(s)
      丹羽明, 平家俊男, 梅田雄嗣, 大嶋宏一, 加藤格, 末盛博文, 中畑龍俊, 斎藤潤
    • Organizer
      日本再生医療学会
    • Place of Presentation
      東京
    • Year and Date
      2011-03-09
    • Related Report
      2011 Final Research Report
  • [Presentation] iPS細胞を用いた先天性造血不全疾患の解析への取り組み2011

    • Author(s)
      丹羽明
    • Organizer
      先天性造血不全シンポジウム
    • Place of Presentation
      浦安
    • Year and Date
      2011-02-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] PRIMITIVE AND DEFINITIVE HEMATOPOIESIS OF HUMAN ES AND IPS CELLS IN SERUM-FREE MONOLAYER CULTURE CONDITION2011

    • Author(s)
      丹羽明
    • Organizer
      ISSCR 8^<th> Annual Meeting
    • Place of Presentation
      サンフランシスコ
    • Year and Date
      2011-02-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト多能性幹細胞を用いた、二次元無血清培養による段階的造血分化誘導2010

    • Author(s)
      丹羽明, 平家俊男, 梅田雄嗣, 大嶋宏一, 加藤格, 末盛博文, 中畑龍俊, 斎藤潤
    • Organizer
      日本血液学会
    • Place of Presentation
      横浜
    • Year and Date
      2010-10-11
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト多能性幹細胞を用いた、二次元無血清培養による段階的造血分化誘導2010

    • Author(s)
      丹羽明
    • Organizer
      第72回日本血液学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-10-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒトES/iPS細胞を用いた、二次元無血清条件による造血幹前駆細胞および成熟血球誘導の試み2010

    • Author(s)
      丹羽明
    • Organizer
      第33回日本造血細胞移植学会
    • Place of Presentation
      松山
    • Year and Date
      2010-10-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒトES/iPS細胞からのin vitro二次元無血清造血誘導における、分化過程の経時的解析2010

    • Author(s)
      丹羽明
    • Organizer
      第31回日本炎症・再生医学会
    • Place of Presentation
      東京
    • Year and Date
      2010-08-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒトES/iPS細胞からのin vitro二次元無血清造血誘導における分化過程の経時的解析2010

    • Author(s)
      丹羽明, 平家俊男, 梅田雄嗣, 大嶋宏一, 加藤格, 末盛博文, 中畑龍俊, 斎藤潤
    • Organizer
      炎症再生医学会
    • Place of Presentation
      東京
    • Year and Date
      2010-07-09
    • Related Report
      2011 Final Research Report
  • [Presentation] In Vitro Orderly Haematopoiesis From Human Pluripotent Cells Traced In a Novel Monolayer Serum-Free Culture2010

    • Author(s)
      Niwa A, Heike T, Umeda K, Oshima K, KatoI, Sakai H, Suemori H, Nakahata T and Saito M
    • Organizer
      American Society of Hematology, 52st Annual Meeting
    • Place of Presentation
      Orlando, Florida, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Primitive and definitive hematopoiesis of human ES and iPS cells in serum-free monolayer culture condition2010

    • Author(s)
      Niwa A, Saito M1, Kato I, Oshima K, Heike T, Nakahata T
    • Organizer
      ISSCR, Annual Meeting
    • Place of Presentation
      SanFrancisco, USA
    • Related Report
      2011 Final Research Report
  • [Book] 幹細胞の体外増幅法の現状と将来医学のあゆみ2012

    • Author(s)
      丹羽明、中畑龍俊
    • Publisher
      医歯薬出版
    • Related Report
      2011 Final Research Report
  • [Book] ES, iPS細胞からの機能的赤血球産生血液フロンティア2009

    • Author(s)
      丹羽明
    • Publisher
      医薬ジャーナル社
    • Related Report
      2011 Final Research Report
  • [Book] iPS細胞からの造血分化Annual Review血液2009

    • Author(s)
      丹羽明, 中畑龍俊
    • Publisher
      中外医学社
    • Related Report
      2011 Final Research Report
  • [Book] 疾患由来iPS細胞の樹立分子細胞治療2009

    • Author(s)
      丹羽明, 中畑龍俊
    • Publisher
      メテオMBC
    • Related Report
      2011 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi