Defective erythropoiesis and ribosomal dysfunction : Analysis of zebrafish model for Diamond-Blackfan anemia.

• Project/Area Number: 22790989
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: University of Miyazaki
• Principal Investigator: UECHI Tamayo 宮崎大学, フロンティア科学実験総合センター, 研究員 (10381104)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: リボソームタンパク質遺伝子 / 造血異常 / ゼブラフィッシュ / リボソームタンパク質 / ダイヤモンド・ブラックファン貧血 / モルフォリノアンチセンスオリゴ / 翻訳異常 / 赤芽球 / リボソーム病

Research Abstract

To investigate the molecular mechanism underlying the pathogenesis of Diamond-Blackfan anemia, we have developed a zebrafish model and carried out microarray analyses to quantify translating mRNAs from polysomal fractions. We found that translational efficiency of some genes required for erythropoiesis had affected.

Research Products

• [Journal Article] Loss of ribosomal RNA modification causes developmental defects in zebrafish (2012)
  • Author(s): Higa-Nakamine S, Suzuki T, Uechi T, Chakraborty A, Nakajima Y, Nakamura M, Hirano N, Suzuki T, Kenmochi N
  • Journal Title: Nucleic Acids Res Volume: 40 Pages: 391-398
  • Peer Reviewed
• [Journal Article] Guarding the 'translation apparatus': defective ribosome biogenesis and the p53 signaling pathway (2011)
  • Author(s): Chakraborty A, Uechi T, Kenmochi N
  • Journal Title: Wiley Interdiscip Rev RNA Volume: 2 Pages: 507-522
  • Peer Reviewed
• [Journal Article] Erythropoiesis failure due to RPS19 deficiency is independent of an activated p53 response in a zebrafish model of Diamond-Blackfan anaemia (2011)
  • Author(s): Torihara H, Uechi T, Chakraborty A, Shinya M, Sakai N, Kenmochi N
  • Journal Title: Br. J. Haematol Volume: 152 Pages: 648-654
  • Peer Reviewed
• [Journal Article] The NPC motif of aquaporin-11, unlike the NPA motif of known aquaporins, is essential for full expression of molecular function (2011)
  • Author(s): Ikeda M, Andoo A, Shimono M, Takamatsu N, Taki A, Muta K, Matsushita W, Uechi T, Matsuzaki T, Kenmochi N, Takata K, Sasaki S, Ito K, Ishibashi K
  • Journal Title: J Biol Chem Volume: 286 Pages: 3342-3350
  • Peer Reviewed
• [Journal Article] Loss of ribosomal RNA modification causes developmental defects in zebrafish (2011)
  • Author(s): Higa-Nakamine S, Suzuki T (第2著者) 他7名
  • Journal Title: Nucleic Acids Research Volume: 40 Issue: 1 Pages: 391-398
  • DOI: 10.1093/nar/gkr700
  • Peer Reviewed
• [Journal Article] Erythropoiesis failure due to RPS19 deficiency is independent of an activated Tp53 response in a zebrafish model of Diamond-Blackfan anaemia. (2011)
  • Author(s): Hidetsugu Torihara
  • Journal Title: The British Journal of Haematology Volume: 152 Pages: 648-654
  • Peer Reviewed
• [Journal Article] The NPC motif of aquaporin-11, unlike the NPA motif of known aquaporins, is essential for full expression of molecular function. (2011)
  • Author(s): Masahiro Ikeda
  • Journal Title: The Journal of Biological Chemistry Volume: 286 Pages: 3342-3350
  • Peer Reviewed
• [Journal Article] Guarding the 'translation apparatus': defective ribosome biogenesis and the p53 signaling pathway.
  • Author(s): Anirban Chakraborty
  • Journal Title: WIREs RNA Volume: (In press)
  • Peer Reviewed
• [Presentation] リボソームの異常と赤血球形成不全:ゼブラフィッシュを用いた解析 (2011)
  • Author(s): 上地珠代
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] ボソームの異常と赤血球形成不全:ゼブラフィッシュを用いた解析 (2011)
  • Author(s): 上地珠代
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Defective erythropoiesis and ribosomal dysfunction in zebrafish model of Diamond-Blackfan anemia (2011)
  • Author(s): Uechi T
  • Organizer: RNA Society Meeting "RNA 2011 Kyoto"
  • Place of Presentation: 京都
  • Year and Date: 2011-06-14
• [Presentation] Defective erythropoiesis and ribosomal dysfunction in zebrafish model of Diamond-Blackfan anemia (2011)
  • Author(s): Tamayo Uechi
  • Organizer: RNA Society Meeting
  • Place of Presentation: 京都
  • Year and Date: 2011-06-14
• [Presentation] ゼブラフィッシュを用いた初期造血異常とリボソームの翻訳調節機構の解析 (2010)
  • Author(s): 上地珠代
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] 造血異常を引き起こすリボソームの翻訳調節機構の解析 (2010)
  • Author(s): 上地珠代
  • Organizer: 日本RNA学会年会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-28
• [Presentation] Ribosomal dysfunction and bone marrow failure : Analysis of zebrafish model for Diamond-Blackfan anemia (2010)
  • Author(s): Uechi T
  • Organizer: 15th Annual Meeting of the RNA Society
  • Place of Presentation: シアトル,米国
  • Year and Date: 2010-06-24
• [Presentation] Ribosomal dysfunction and bone marrow failure : Analysis of zebrafish model for Diamond-Blackfan anemia. (2010)
  • Author(s): Tamayo Uechi
  • Organizer: 15th Annual Meeting of the RNA Society
  • Place of Presentation: シアトル、米国
  • Year and Date: 2010-06-24