Diclofenac enhances proinflammatory cytokine-induced phagocytosis through nitric oxide production in cultured microglia

• Project/Area Number: 22790993
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: Nagoya City University
• Principal Investigator: KAKITA Hiroki 名古屋市立大学, 大学院・医学研究科, 助教 (40528949)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: インフルエンザ脳症 / グリア細胞 / ジクロフェナック / 一酸化窒素 / ミクログリア / 血液脳関門 / アストロサイト / アクアポリン

Research Abstract

this study demonstrates that iNOS and NO are induced in microglia cultures by proinflammatory cytokines. The addition of DCF further augments NO production. This effect is mediated via NF-B signaling and induced microglia activity and phagocytosis.

Research Products

• [Journal Article] Hypothermia attenuates the severity of oxidative stress development in asphyxiated newborns (2012)
  • Author(s): Kakita H, et al
  • Journal Title: J Crit Care Volume: (In press)
  • Peer Reviewed
• [Journal Article] Endogenous erythropoietin from astrocyte protects the oligodendrocyte precursor cell against hypoxic and reoxygenation injury (2011)
  • Author(s): Kato S, Aoyama M, Hida, H, et al. 11名4番目
  • Journal Title: J Neurosci. Res. Volume: 89 Issue: 10 Pages: 1566-74
  • DOI: 10.1002/jnr.22702
  • Peer Reviewed
• [Journal Article] Endogenous erythropoietin from astrocyte protects the oligodendrocyte precursor cell against hypoxic and reoxygenation injury (2011)
  • Author(s): Kato S, Kakita H, et al
  • Journal Title: J Neurosci Res Volume: 89 Pages: 1566-1574
  • Peer Reviewed
• [Journal Article] Atypical social development in NICU survivors at 12 months (2011)
  • Author(s): Yamda Y, Kakita H, et al
  • Journal Title: Pediatr Int Volume: 53 Pages: 858-866
  • Peer Reviewed
• [Journal Article] Atypical social development in NICU survivors at 12 months. (2011)
  • Author(s): Yamada Y
  • Journal Title: Pediatrics international Volume: (in Press)
  • Peer Reviewed
• [Journal Article] Edaravone, a hydroxyl radical scavenger, ameliorates the severity of pulmonary hypertension in a porcine model of neonatal sepsis. (2011)
  • Author(s): Yamaguchi S
  • Journal Title: Tohoku J Exp Med Volume: 223 Pages: 235-241
  • Peer Reviewed
• [Journal Article] Oxidative stress after living related liver transplantation subsides with time in pediatric patients (2011)
  • Author(s): Hussein MH
  • Journal Title: Pediatr Surg Int Volume: 27 Pages: 17-22
  • Peer Reviewed
• [Journal Article] Endothelin receptor antagonist attenuates inflammatory response and prolongs the survival time in a neonatal sepsis model. (2010)
  • Author(s): Goto T
  • Journal Title: Intensive Care Med Volume: 36 Pages: 2132-9
  • Peer Reviewed
• [Journal Article] Endothelin receptor antagonist attenuates inflammatory response and prolongs the survival time in a neonatal sepsis model. (2010)
  • Author(s): Hussein MH
  • Journal Title: Free Radic Res Volume: 44 Pages: 422-9
  • Peer Reviewed
• [Journal Article] Effect of polymyxin B-immobilized fiber hemoperfusion on respiratory impairment, hepatocellular dysfunction, and leucopenia in a neonatal sepsis model. (2010)
  • Author(s): Hussein MH
  • Journal Title: Pediatr Surg Int Volume: 26 Pages: 187-93
  • Peer Reviewed
• [Journal Article] Atypical social development in NICU survivors at 12 months
  • Author(s): Yamada Y, Yoshida F, Hemmi H, Ito M, Kakita H, Yoshikawa T, Hishida M, Iguchi T, Seo T, Nakanishi K
  • Journal Title: Pediatr Int Volume: (in press)
  • Peer Reviewed
• [Presentation] アストロサイト由来エリスロポエチンがオリゴデンドロサイト前駆細胞に与える影響 (2011)
  • Author(s): 垣田博樹
  • Organizer: 第47回日本周産期・新生児学会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-07-11
• [Presentation] アストロサイト由来エリスロポエチンがオリゴデンドロサイト前駆細胞に与える影響 (2011)
  • Author(s): 垣田博樹, 他
  • Organizer: 第47回日本周産期新生児学会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] 脳低温療法が酸化ストレスマーカーに及ぼす影響についての検討 (2010)
  • Author(s): 垣田博樹
  • Organizer: 第55回日本未熟児新生児学会
  • Place of Presentation: 神戸(国際会議場)
• [Presentation] ジクロフェナックによるNFκBシグナルを介したアストロサイトでのNOx産生増強 (2010)
  • Author(s): 垣田博樹
  • Organizer: 第113回日本小児科学会
  • Place of Presentation: 盛岡(市民文化ホール)
• [Presentation] Diclofenac has a synergistic effect on the production of nitric oxide through NF-κB signaling in cultured astrocytes stimulated by proinflammatory cytokines (2010)
  • Author(s): 青山峰芳
  • Organizer: 第53回日本神経化学会大会
  • Place of Presentation: 神戸(国際会議場)
• [Remarks]
  • URL: http://www.med.nagoya-cu.ac.jp/w3med/research/reports/results/002.html
• [Remarks]
• [Remarks]
  • URL: http://www.med.nagoya-cu.ac.jp/w3med/research/reports/results/002.html