Identification of tumor stem-specific transcription factor in neuroblastoma sphere

• Project/Area Number: 22791017
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: Chiba Cancer Center (Research Institute)
• Principal Investigator: TAKENOBU Hisanori 千葉県がんセンター(研究所), 発がん制御研究部, 研究員 (60392247)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 幹細胞 / 発がん / 分化

Research Abstract

We reported that CD133 suppressed differentiation in NB cells and CD133 was up-regulated in NB sphere (ONCOGENE, 2011). To identify the sphere specific transcription factors, we examined expression of several transcription factors in NB spheres. The expression pattern of the TFX transcription factor was correlated with that of CD133 during NB tumor sphere formation. Furthermore, TFX directly bound to CD133 promoter region and up-regulated CD133 expression, cell proliferation and NB sphere forming efficiency. These results suggest that tumor sphere specific transcription factor TFX may contribute to maintenance of tumor initiating cells and their characteristics.

Research Products

• [Journal Article] CD133 suppresses neuroblastoma cell differentiation via signal pathway modification (2011)
  • Author(s): Takenobu H, Shimozato 0, Nakamura T, Ochiai H, Yamaguchi Y, Ohira M, Nakagawara A, Kamijo T
  • Journal Title: Oncogene 2011 Volume: 6;30(1) Pages: 97-105
• [Journal Article] CD133 suppresses neuroblastoma cell differentiation via signal pathway modification (2011)
  • Author(s): Takenobu H, Shimozato O, Nakamura T, Ochiai H, Yamaguchi Y, Ohira M, Nakagawara A, Kamijo T
  • Journal Title: Oncogene Volume: 30 Issue: 1 Pages: 97-105
  • DOI: 10.1038/onc.2010.383
  • Peer Reviewed
• [Journal Article] Bmi1 regulates cell fate via tumor suppressor WWOX repression in small cell lung cancer cells (2011)
  • Author(s): Kimura M, Takenobu H, Akita N, Nakazawa A, Ochiai H, Shimozato O, Fujimura YI, Koseki H, Yoshino I, Kimura H, Nakagawara A, Kamijo T
  • Journal Title: Cancer Sci Volume: 102 Issue: 5 Pages: 983-990
  • DOI: 10.1111/j.1349-7006.2011.01891.x
  • NAID: 10029293384
  • Peer Reviewed
• [Journal Article] CD133 suppresses neuroblastoma cell differentiation via signal pathway modification. (2011)
  • Author(s): Takenobu H, Shimozato O, Nakamura T, Ochiai H, Yamaguchi Y, Ohira M, Nakagawara A, Kamijo T.
  • Journal Title: Oncogene Volume: 30 Pages: 97-105
  • Peer Reviewed
• [Journal Article] Bmi1 regulates cell fate via tumor suppressor WWOX repression in small-cell lung cancer cells. (2011)
  • Author(s): Kimura M, Takenobu H, Akita N, Nakazawa A, Ochiai H, Shimozato O, Fujimura YI, Koseki H, Yoshino I, Kimura H, Nakagawara A, Kamijo T.
  • Journal Title: Cancer Science Volume: 102 Pages: 983-990
  • NAID: 10029293384
  • Peer Reviewed
• [Journal Article] Bmil is a MYCN target gene that regulates tumorigenesis through repression of KIF1Bbeta and TSLC1 in neuroblastoma. (2010)
  • Author(s): Ochiai H, Takenobu H, Nakagawa A, Yamaguchi Y, Kimura M, Ohira M, Okimoto Y, Fujimura Y, Koseki H, Kohno Y, Nakagawara A, Kamijo T.
  • Journal Title: Oncogene Volume: 29 Pages: 2681-2690
  • Peer Reviewed
• [Journal Article] Polycomb group molecule PHC3 regulates polycomb complex composition and prognosis of osteosarcoma (2010)
  • Author(s): Iwata S, Takenobu H, Kageyama H, Koseki H, Ishii T, Nakazawa A, Tatezaki S, Nakagawara A, Kamijo S.
  • Journal Title: Cancer Science Volume: 101 Pages: 1646-1652
  • NAID: 10026594687
  • Peer Reviewed
• [Journal Article] HDM2 impairs Noxa transcription and affects apoptotic cell death in a p53/p73-dependent manner in neuroblastoma. (2010)
  • Author(s): Shi Y, Takenobu H, Kurata K, Yamaguchi Y, Yanagisawa R, Ohira M, Koike K, Nakagawara A, Jiang LL, Kamijo T.
  • Journal Title: European Journal of Cancer Volume: 46 Pages: 2324-2334
  • Peer Reviewed
• [Journal Article] Polycomb group molecule PHC3 regulates polycomb complex composition and prognosis of osteosarcoma
  • Author(s): Iwata S, Takenobu H, Kageyama H, Koseki H, Ishii T, Nakazawa A, Tatezaki S, Nakagawara A, Kamijo T
  • Journal Title: Cancer Sci Volume: 101(7) Pages: 1646-1652
  • NAID: 10026594687
• [Journal Article] HDM2 impairs Noxa transcription and affects apoptotic cell death in a p53/p73-dependentmanner in neuroblastoma
  • Author(s): Shi Y, Takenobu H, Kurata K, Yamaguchi Y, Yanagisawa R, Ohira M, Koike K, Nakagawara A, Jiang LL, Kamijo T
  • Journal Title: Eur J Cancer Volume: 46(12) Pages: 2324-2334
• [Journal Article] Bmil regulates cell fate via tumor suppressor WWOX repression in small-cell lung cancer cells
  • Author(s): Kimura M, Takenobu H, Akita N, Nakazawa A, Ochiai H, Shimozato 0, Fujimura Y, Koseki H, Yoshino I, Kimura H, Nakagawara A, Kamijo T
  • Journal Title: Cancer Sci Volume: 102(5) Pages: 983-990
• [Presentation] Tumor sphere specific CD133 regulation in neuroblastoma (2012)
  • Author(s): 竹信尚典,下里修,秋田直洋,山口陽子,上原悠,藁谷美雪,吉田早哉香,落合秀匡,大平美紀,中川原章,上條岳彦
  • Organizer: Advances of Neuroblastoma Research 2012
  • Place of Presentation: トロント
• [Presentation] Tumor sphere specific CD 133regulation in neuroblastoma (2012)
  • Author(s): 竹信尚典,下里修,秋田直洋,山口陽子,上原悠,藁谷美雪,吉田早哉香,落合秀匡,大平美紀,中川原章,上條岳彦
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] Identification of key molecules that regulate neuroblastoma sphere formation (2012)
  • Author(s): 竹信尚典,下里修,秋田直洋,山口陽子,上原悠,藁谷美雪,吉田早哉香,落合秀匡,大平美紀,中川原章,上條岳彦
  • Organizer: 第54回日本小児血液・がん学会学術集会
  • Place of Presentation: 横浜
• [Presentation] CD133 suppresses neuro-blastoma cell differentiation via signal pathway modification, (2012)
  • Author(s): 竹信尚典
  • Organizer: 第54回日本小児血液・がん学会学術集会
  • Place of Presentation: 横浜
• [Presentation] MYCN-DEPENDENT ONCOGENIC BMI1 INDUCTION IN NEUROBLA STOMA AND FIBROBLASTS (2012)
  • Author(s): Hidemasa Ochiai, Hisanori Takenobu, Yohko Yamaguchi, Nobuhiro Akita, Takehiko Kamijo
  • Organizer: 第7回 International Society of Paediatric Oncology (SIOP) Asia 2012
  • Place of Presentation: ジョクジャカルタ(インドネシア)
• [Presentation] Functional analysis of a novel 1p tumor suppressor DMAP1 (2012)
  • Author(s): Yohko Yamaguchi, Hisanori Takenobu, Sayaka Yoshida, Nobuhiro Akita, Miki Ohira, Atsuko Nakazawa, Akira Nakagawara, Takehiko Kamijo
  • Organizer: Advances of Neuroblastoma Research 2012
  • Place of Presentation: トロント(カナダ)
• [Presentation] Tumor sphere specific CD133 regulation in neuroblastoma (2012)
  • Author(s): Hisanori Takenobu, Osamu Shimozato, Nobuhiro Akita, Yohko Yamaguchi, Haruka Uehara, Miyuki Waraya, Sayaka Yoshida, Akira Nakagawara, Takehiko Kamijo
  • Organizer: Advances of Neuroblastoma Research 2012
  • Place of Presentation: トロント(カナダ)
• [Presentation] BMI1 regulates neuroblastoma apoptotic cell death and differentiation (2012)
  • Author(s): Nobuhiro Akita, Hisanori Takenobu, Atsuko Nakazawa, Hidemasa Ochiai, Akira Nakagawara, Takehiko Kamijo
  • Organizer: Advances of Neuroblastoma Research 2012
  • Place of Presentation: トロント(カナダ)
• [Presentation] The role of Cancer Stem Cell marker CD133 in tumorigenesis (2012)
  • Author(s): Takehiko Kamijo
  • Organizer: Japan-China Symposium on Cancer Research 2012
  • Place of Presentation: 千葉
• [Presentation] Functional analysis of a novel 1p tumor suppressor DMAP1 (2012)
  • Author(s): Yohko Yamaguchi, Hisanori Takenobu, Sayaka Yoshida, Nobuhiro Akita, Miki Ohira, Akira Nakagawara, Takehiko Kamijo
  • Organizer: Japan-China Symposium on Cancer Research 2012
  • Place of Presentation: 千葉
• [Presentation] ANALYSIS OF MYCN-DEPENDENT APOPTOTIC CELL DEATH PATHWAY (2012)
  • Author(s): Yohko Yamaguchi, Hisanori Takenobu, Nobuhiro Akita, Takehiko Kamijo
  • Organizer: 第44回International Society of Paediatric Oncology (SIOP) 2012
  • Place of Presentation: ロンドン(イギリス)
• [Presentation] Tumor sphere specific CD133 regulation in neuroblastoma (2012)
  • Author(s): Hisanori Takenobu, Osamu Shimozato, Nobuhiro Akita, Yohko Yamaguchi, Haruka Uehara, Miyuki Waraya, Sayaka Yoshida, Akira Nakagawara, Takehiko Kamijo
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] Sphere formation produces multi-drug resistance in humancolon cancer cells resulting via histone modification of ABCB1promoter (2012)
  • Author(s): Osamu Shimozato, Haruka Uehara, Miyuki Waraya, Hisanori Takenobu, Akira Nakagawara , Takehiko Kamijo
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] BMI1 regulates neuroblastoma apoptotic cell death and differentiation (2012)
  • Author(s): Nobuhiro Akita, Hisanori Takenobu, Hidemasa Ochiai, Atsuko Nakazawa, Akira Nakagawara, Takehiko Kamijo
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] Polycomb group protein BMI1 prevents neuroblastoma cells from DNA-damage-induced apoptotic cell death (2012)
  • Author(s): Nobuhiro Akita, Hisanori Takenobu, Hidemasa Ochiai, Atsuko Nakazawa, Akira Nakagawara, Takehiko Kamijo
  • Organizer: 第54回日本小児血液・がん学会学術集会
  • Place of Presentation: 横浜
• [Presentation] CD133 suppresses neuroblastoma cell differentiation via signal pathway modification (2012)
  • Author(s): Hisanori Takenobu
  • Organizer: 第54回日本小児血液・がん学会学術集会
  • Place of Presentation: 横浜
• [Presentation] DMAP1 acts as a tumor suppressor through ATM activation in neuroblastoma various regulation mechanisms of ATM family kinases and their tissue- or species-specific roles (2012)
  • Author(s): Yohko Yamaguchi, Hisanori Takenobu, Sayaka Yoshida, Nobuhiro Akita, Miki Ohira, Atsuko Nakazawa, Akira Nakagawara, Takehiko Kamijo
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 1番染色体短腕に位置するがん抑制遺伝子DMAP1の転写制御機構の解析 (2012)
  • Author(s): Sayaka Yoshida, Yohko Yamaguchi, Hisanori Takenobu, Akira Nakagawara, Takehiko Kamijo
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 幹細胞マーカーCD133は細胞内シグナル活性化を介して神経芽腫の増殖と分化を制御する (2011)
  • Author(s): 竹信尚典,上條岳彦
  • Organizer: 第116回小児血液腫瘍懇話会
  • Place of Presentation: 東京
• [Presentation] CD133 plays roles in keeping the undifferentiated status of human colon cancer cells via PI3K/AKT activation (2011)
  • Author(s): 竹信尚典,下里修,秋田直洋,山口陽子,吉田早哉香,中川原章,上條岳彦
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] 神経芽腫/脳腫瘍スフェア特異的なCD133発現調節機構 (2011)
  • Author(s): 竹信尚典,下里修,秋田直洋,山口陽子,吉田早哉香,落合秀匡,大平美紀,中川原章,上條岳彦
  • Organizer: 第53回日本小児血液・がん学会
  • Place of Presentation: 前橋
• [Presentation] Bmi1 regulates cell fate via tumor suppressor WWOX repression in small cell lung cancer cells (2011)
  • Author(s): Masaki Kimura, Hisanori Takenobu, Nobuhiro Akita, Osamu Shimozato, Hideki Kimura, Akira Nakagawara, Takehiko Kamijo
  • Organizer: AACR 102nd Annual Meeting 2011
  • Place of Presentation: フロリダ(アメリカ)
• [Presentation] MDM2 impairs NOXA transcription and affects apoptotic cell death in a p53/p73-dependent manner in neuroblastoma (2011)
  • Author(s): Yun Shi, Hisanori Takenobu, Kenji Kurata, Yohko Yamaguchi, Ken-ichi Koike, Akira Nakagawara, Takehiko Kamijo
  • Organizer: The 5 th International p63/p73 Workshop
  • Place of Presentation: リヨン(フランス)
• [Presentation] DMAP1 works as a novel 1p tumor suppressor via ATM/p53 activation (2011)
  • Author(s): Yohko Yamaguchi, Hisanori Takenobu, Sayaka Yoshida, Miki Ohira, Atsushi Iwama, Akira Nakagawara, Takehiko Kamijo
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] BMI1 regulates neuroblastoma apoptotic cell death and differentiation (2011)
  • Author(s): Nobuhiro Akita, Hisanori Takenobu, Hiroki Nagase, Takehiko Kamijo
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Acquired multi-drug resistance in human colon cancer cells by sphere formation (2011)
  • Author(s): Kumiko Nakashima, Osamu Shimozato, Hisanori Takenobu, Toshinori Ozaki, Akira Nakagawara, Takehiko Kamijo
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] BMI1発現抑制による難治性神経芽腫の細胞死・分化誘導療法の開発 (2011)
  • Author(s): 秋田直洋, 竹信尚典, 落合秀匡, 永瀬浩喜, 中川原章, 上條岳彦
  • Organizer: 第53回日本小児血液・がん学会
  • Place of Presentation: 前橋
• [Presentation] CD133はシグナル経路依存的に受容体RETの転写抑制を引き起こし、神経芽腫の増殖と分化を制御する (2010)
  • Author(s): 竹信尚典, 下里修, 山口陽子, 秋田直洋, 吉田早哉香, 落合秀匡, 大平美紀, 中川原章, 上條岳彦
  • Organizer: 第26回日本小児がん学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-12-18
• [Presentation] CD133 regulates neuroblastoma stemness via p38MAPK and PI3K/Akt activation (2010)
  • Author(s): 竹信尚典, 下里修, 落合秀匡, 山口陽子, 大平美紀, 中川原章, 上條岳彦
  • Organizer: 第69回 日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-23
• [Presentation] CD133 regulates signal transduction pathways and prevents differentiation via RET suppression in neuroblastoma cells (2010)
  • Author(s): 竹信尚典, 下里修, 落合秀匡, 山口陽子, 大平美紀, 中川原章, 上條岳彦
  • Organizer: Advances of Neurobiastoma Research 2010
  • Place of Presentation: ストックホルム
  • Year and Date: 2010-06-22
• [Presentation] CD133 regulates signal transduction pathways and prevents differentiation via RET suppression in neuroblastoma cells (2010)
  • Author(s): 竹信尚典,下里修,落合秀匡,山口陽子,大平美紀,中川原章,上條岳彦
  • Organizer: Advances of Neuroblastoma Research 2010
  • Place of Presentation: ストックホルム
• [Presentation] CD133 regulates neuroblastoma stemness via p38MAPK and PI3K/Akt activation (2010)
  • Author(s): 竹信尚典,下里修,落合秀匡,山口陽子,大平美紀,中川原章,上條岳彦
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] CD133はシグナル経路依存的に受容体RETの転写抑制を引き起こし、神経芽腫の増殖と分化を制御する (2010)
  • Author(s): 竹信尚典,下里修,山口陽子,秋田直洋,吉田早哉香,落合秀匡,大平美紀,中川原章,上條岳彦
  • Place of Presentation: 大阪
• [Remarks]
  • URL: http://www.chiba-cc.jp/inst/jp/organization/005.htmlhttp://kamijo-labo.jp/
• [Remarks]
  • URL: http://www.chiba-cc.jp/inst/jp/organization/005.html
• [Remarks]
  • URL: http://kamijo-labo.jp
• [Remarks]
  • URL: http://www.chiba-cc.jp/inst/jp/organization/005.html
• [Remarks]
  • URL: http://kamijo-labo.jp/