Development of antiviral treatment for paramyxovirus infection that target the L protein

Research Project

Project/Area Number	22791018
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Pediatrics
Research Institution	National Institute of Infectious Diseases
Principal Investigator	SEKI Fumio 国立感染症研究所, ウイルス第三部, 主任研究官 (20443111)
Project Period (FY)	2010 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords	小児感染症 / 麻疹ウイルス / Pタンパク質 / Lタンパク質 / タンパク質発現
Research Abstract	P protein with a deletion of amino acids between 307 and 336 (Pd307-336) decreased the interaction between P and P, and P and L protein. Expressing the Pd307-336 inhibited the virus growth. Similar effects were obtained when lacking the N-terminal amino acids of the Pd307-336. I was expressed L protein and mutant P proteins using Escherichia coli as a host.