Analysis of risk factors to be symptomatic congenital cytomegalovirus infection

• Project/Area Number: 22791034
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Fukushima Medical University
• Principal Investigator: IKUTA Kazufumi 福島県立医科大学, 医学部微生物学講座, 助教 (60512184)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 先天感染 / サイトメガロウイルス / 血清疫学 / ELISA 法 / リアルタイム PCR 法 / 先天性感染 / ELISA法 / リアルタイムPCR法

Research Abstract

We established Cytomegalovirus (CMV) strain-specific real time PCR, and found that only a single genotype determined by CMV gH neutralizing epitope was found in the urine from neonates with congenital CMV infection, even though the case possessed antibodies against multiple CMV strains. Our results showed that CMV reinfection also caused congenital CMV infection.

Research Products

• [Journal Article] Herpes simplex virus type 1 virionderived US11 inhibits type 1 interferon-induced protein kinase R phosphorylation. (2013)
  • Author(s): Ishioka K, Ikuta K, Sato Y, Kaneko H, Sorimachi K, Fukushima E, Saijo M, Suzutani T
  • Journal Title: Microbiol Immunol
  • Peer Reviewed
• [Journal Article] Anti influenza virus activity of two extracts of the Blackcurrant (Ribes nigrum L.) from New Zealand and Poland (2013)
  • Author(s): Ikuta K, Mizuta K, Suzutani T
  • Journal Title: Fukushima J Med Sci
  • Peer Reviewed
• [Journal Article] A case report of a patient in whom antibodies against the 2009 pandemic influenza A/H1N1 virus have been present since June 1999 (2013)
  • Author(s): Yoshida M, Sugino H, Iizuka T, Xiaofang L, Suzuki A, Oshitani H, Suzutani T, Ikuta K
  • Journal Title: Hum Vaccin Immunother
  • Peer Reviewed
• [Journal Article] Relationship between polyphenol content and anti-influenza viral effects in Berries (2013)
  • Author(s): Sekizawa H, Ikuta K, Mizuta K, Takechi S, Suzutani T
  • Journal Title: J Sci Food Agric
  • Peer Reviewed
• [Journal Article] Relationship between polyphenol content and anti-influenza viral effects in Berries. (2013)
  • Author(s): Sekizawa H, Ikuta K, Mizuta K, Takechi S, Suzutani T.
  • Journal Title: Journal of the Science of Food and Agriculture Volume: in press
  • Peer Reviewed
• [Journal Article] Case in which antibody against 2009 pandemic influenza A/H1N1 virus has been present since June 1999. (2013)
  • Author(s): Yoshida M, Sugino H, Iizuka T, Xiaofang L, Suzuki A, Oshitani H, Suzutani T, Ikuta K.
  • Journal Title: Human Vaccines and Immunotherapeutics Volume: in press
  • Peer Reviewed
• [Journal Article] Anti influenza virus activity of two extracts of the Blackcurrant (Ribes nigrum L.) from New Zealand and Poland. (2013)
  • Author(s): Ikuta K, Mizuta K, Suzutani T.
  • Journal Title: Fukushima Journal of Medical Science Volume: in press
  • Peer Reviewed
• [Journal Article] Herpes simplex virus type 1 virion-derived US11 inhibits type 1 interferon-induced protein kinase R phosphorylation. (2013)
  • Author(s): Ishioka K, Ikuta K, Sato Y, Kaneko H, Sorimachi K, Fukushima E, Saijo M, Suzutani T.
  • Journal Title: Microbiology and Immunology Volume: in press
  • Peer Reviewed
• [Journal Article] Anti-viral and anti-bacterial activities of an extract of the Blackcurrant (Ribes nigrum L.) (2012)
  • Author(s): Ikuta K, Hashimoto K, Kaneko H, Mori S, Ohashi K, Suzutani T.
  • Journal Title: Microbiol Immunol Volume: 56(12) Pages: 805-9
  • Peer Reviewed
• [Journal Article] A novel real-time PCR method for the determination and quantification of each cytomegalovirus (CMV) gH-subtype in clinical samples (2012)
  • Author(s): Ikuta K, Ishioka K, Sato Y, Imamura T, Asano K, Koyano S, Inoue N, Suzutani T
  • Journal Title: J Clin Microbiol Volume: 50(2) Pages: 499-501
  • Peer Reviewed
• [Journal Article] Anti-viral and anti-bacterial activities of an extract of the Blackcurrant (Ribes nigrum L.) (2012)
  • Author(s): Ikuta K, Hashimoto K, Kaneko H, Mori S, Ohashi K, Suzutani T.
  • Journal Title: Microbiology and Immunology Volume: 56 Pages: 805-809
  • Peer Reviewed
• [Journal Article] A novel real-time PCR method for the determination and quantification of each cytomegalovirus (CMV)gH-subtype in clinical samples (2012)
  • Author(s): Ikuta K, Ishioka K, Sato Y, Imamura T, Asano K, Koyano S, Inoue N, Suzutani T
  • Journal Title: Journal of Clinical Microbiology Volume: 2 Pages: 499-501
  • Peer Reviewed
• [Journal Article] Japanese Congenital Cytomegalovirus Study Group (2011)
  • Author(s): Koyano S, Inoue N, Oka A, Moriuchi H, Asano K, Ito Y, Yamada H, Yoshikawa T, Suzutani T
  • Journal Title: Screening for congenital cytomegalovirus infection using newborn urine samples collected on filter paper: feasibility and outcomes from a multicentre studyBMJ Open Volume: 1(1)
  • Peer Reviewed
• [Journal Article] Epithelial cell retention of transcriptionally active, P3HR-1-derived heterogeneous (het) EBV DNA with concurrent loss of parental virus (2011)
  • Author(s): Ikuta K, Ding M, Zhang F, Sixbey JW, Scott RS
  • Journal Title: J Virol Volume: 85(15) Pages: 7634-43
  • Peer Reviewed
• [Journal Article] Inoue N and Hosoya M. Oral Valganciclovir treatment for congenital (2011)
  • Author(s): Imamura T, Suzutani T, Ogawa H, Asano K, Nomoto M, Matsui T, Momoi M, Ikuta K
  • Journal Title: Cytomegalovirus infectionPediatr Int. Volume: 53(2) Pages: 249-52
  • Peer Reviewed
• [Journal Article] Lack of antibodies against the AD2 epitope of cytomegalovirus (CMV) glycoprotein B (gB) is associated with CMV disease after renal transplantation in recipients having gH serotypes same as their donors (2011)
  • Author(s): Ishibashi K, Tokumoto T, ShirakawaH, Hashimoto K, Ikuta K, Kushida N, Yanagida T, Shishido K, Aikawa K, Toma H, Inoue N, Yamaguchi O, TanabeK, Suzutani T
  • Journal Title: Transpl Infect Dis Volume: 13(3) Pages: 318-23
  • Peer Reviewed
• [Journal Article] 腎移植の急性拒絶反応とサイトメガロウイルス (2011)
  • Author(s): 錫谷達夫、石橋 啓、生田和史
  • Journal Title: 今日の移植 Volume: 第24巻 第4号 Pages: 369-377
• [Journal Article] Oral Valganciclovir treatment for congenital Cytomegalovirus infection (2011)
  • Author(s): Imamura T, Suzutani T, Ogawa H, Asano K, Nomoto M, Matsui T, Momoi M, Ikuta K, Inoue N, Hosoya M
  • Journal Title: Pediatrics International Volume: 2 Pages: 249-52
  • Peer Reviewed
• [Journal Article] Epithelial cell retention of transcriptionally active, P3HR-1-derived heterogeneous(het)EBV DNA with concurrent loss of parental virus (2011)
  • Author(s): Ikuta K, Ding M, Zhang F, Sixbey JW, Scott RS
  • Journal Title: Journal of Virology Volume: 15 Pages: 7634-43
  • Peer Reviewed
• [Journal Article] Screening for congenital cytomegalovirus infection using newborn urine samples collected on filter paper : feasibility and outcomes from a multicentre study (2011)
  • Author(s): Koyano S, Inoue N, Oka A, Moriuchi H, Asa no K, Ito Y,Yamada H, Yoshikawa T, Suzutani T, Japanese Congenital Cytomegalovirus study Group
  • Journal Title: BMJ Open Volume: 1
  • Peer Reviewed
• [Journal Article] 腎移植の急性拒絶反応とサイトメガロウイルス (2011)
  • Author(s): 錫谷達夫、石橋啓、生田和史
  • Journal Title: 今日の移植 Volume: 24 Pages: 369-377
• [Journal Article] The lack of antibodies against the AD2 epitope of cytomegalovirus (CMV) glycoprotein B (gB) is associated with CMV disease after renal transplantation in recipients having gH serotypes same as their donors. (2010)
  • Author(s): Ishibashi K, Tokumoto T, Shirakawa H, Hashimoto K, Ikuta K, Kushida N, Yanagida T, Shishido K, Aikawa K, Toma H, Inoue N, Yamaguchi O, Tanabe K, Suzutani T.
  • Journal Title: Transplant Infectious Disease Volume: Aug 30
  • Peer Reviewed
• [Presentation] 聴覚障害を引き起こすサイトメガロウイルス感染:モデルマウスを用いた解析 (2012)
  • Author(s): 生田和史、小川洋、新井義文、小杉伊三夫、大森孝一、錫谷達夫
  • Organizer: 第66回日本細菌学会東北支部会総会
  • Place of Presentation: 仙台
• [Presentation] A genotypic and serologic study of Cytomegalovirus (CMV) reinfection in mothers and neonates with congenital CMV infection in Japan (2012)
  • Author(s): Kazufumi Ikuta, Ken Ishioka, Takashi Imamura, Kimisato Asano, Tetsushi Yoshikawa, Hiroyuki Moriuchi, Shigeyoshi Fujiwara, Takahiko Kubo, Shin Koyano, Naoki Inoue and Tatsuo Suzutani
  • Organizer: International Union of Microbiological Societies 2011CongressesXV, International Congress of Virology
  • Place of Presentation: Sapporo
• [Presentation] 聴覚障害を引き起こすサイトメガロウイルス感染：モデルマウスを用いた解析 (2012)
  • Author(s): 生田和史、小川洋、新井義文、小杉伊三夫、大森孝一、錫谷達夫
  • Organizer: 第６６回日本細菌学会東北支部会総会
  • Place of Presentation: 仙台
• [Presentation] 先天性サイトメガロウイルス感染におけるウイルス抗体価ならびに DNA 量の型別解析 (2011)
  • Author(s): 生田和史、石岡 賢、佐藤友香、石橋啓、浅野仁覚、今村 孝、藤原成悦、久保隆彦、中井英剛、吉川哲史、森内浩幸、古谷野伸、井上直樹、錫谷達夫
  • Organizer: 第65回日本細菌学会東北支部会総会
  • Place of Presentation: 山形
• [Presentation] リアルタイムPCR法によるサイトメガロウイルスの型別定量判別 (2011)
  • Author(s): 生田和史、石岡 賢、佐藤友香、石橋啓、浅野仁覚、今村 孝、藤原成悦、久保隆彦、中井英剛、吉川哲史、森内浩幸、古谷野伸、井上直樹、錫谷達夫
  • Organizer: 第26回 ヘルペスウイルス研究会
  • Place of Presentation: 大阪
• [Presentation] リアルタイムPCR法によるサイトメガロウイルスの型別定量判別 (2011)
  • Author(s): 生田和史、石岡賢、佐藤友香、石橋啓、浅野仁覚、今村孝、藤原成悦、久保隆彦、中井英剛、吉川哲史、森内浩幸、古谷野伸、井上直樹、錫谷達夫
  • Organizer: 第26回ヘルペスウイルス研究会
  • Place of Presentation: 大阪
• [Presentation] 先天性サイトメガロウイルス感染におけるウイルス抗体価ならびにDNA量の型別解析 (2011)
  • Author(s): 生田和史、石岡賢、佐藤友香、石橋啓、浅野仁覚、今村孝、藤原成悦、久保隆彦、中井英剛、吉川哲史、森内浩幸、古谷野伸、井上直樹、錫谷達夫
  • Organizer: 第65回日本細菌学会東北支部会総会
  • Place of Presentation: 山形
• [Presentation] A genotypic and serologic study of Cytomegalovirus (CMV) reinfection in mothers and neonates with congenital CMV infection in Japan (2011)
  • Author(s): Kazufumi Ikuta, Ken Ishioka, Takashi Imamura, Kimisato Asano, Tetsushi Yoshikawa, Hiroyuki Moriuchi, Shigeyoshi Fujiwara, Takahiko Kubo, Shin Koyano, Naoki Inoue, Tatsuo Suzutani
  • Organizer: International Union of Microbiological Societies 2011 Congresses XV, International Congress of Virology
  • Place of Presentation: 札幌
• [Presentation] リアルタイムPCR法を用いたサイトメガロウイルスの型別定量判定法 (2010)
  • Author(s): 生田和史、石岡賢、佐藤友香、金子久俊、古谷野伸、井上直樹、錫谷達夫
  • Organizer: 第58回日本ウイルス学会
  • Place of Presentation: 徳島
• [Presentation] 先天性サイトメガロウイルス感染児の母親のウイルス型別抗体検出法を用いた感染パターンの解析 (2010)
  • Author(s): 生田和史、今村孝、浅野仁覚、古谷野伸、藤原成悦、中井英剛、吉川哲史、森内浩幸、井上直樹、錫谷達夫
  • Organizer: 第25回ヘルペスウイルス研究会
  • Place of Presentation: 浜松