| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
Abnormal morphogenesis of the neural tube leads to neural tube defects including spina bifida and anencephaly. We have revealed that WGEF, a novel guanine nucleotide exchanging factor, mediates Wnt-PCP pathway during morphogenic movements of neural tube in the amphibian embryo. In this study, I attempted to generate a conditional knockout mouse of WGEF gene as a mammalian model of neural tube defects. So far, several conditional knockout allele on the WGEF locus was introduced by homologous recombination in the ES cells. Using this ES cell lines, chimera mice carrying WGEF conditional knockout cells have been generated.
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