Regulation of morphogenetic movements by a guanine nucleotide-exchange factor involved in Wnt-PCP pathway
| Project/Area Number |
22791043
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | 公益財団法人東京都医学総合研究所 (2012)
Tokyo Metropolitan Organization for Medical Research (2010-2011) |
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Principal Investigator |
TANEGASHIMA Kosuke 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 主任研究員 (20507678)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 先天異常学 / 神経管閉鎖障害 / 発生・分化 / 脳・神経 / ノックアウトマウス / WGEF / シグナル伝達 / ES細胞 / 相同組み替え |
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| Research Abstract |
Abnormal morphogenesis of the neural tube leads to neural tube defects including spina bifida and anencephaly. We have revealed that WGEF, a novel guanine nucleotide exchanging factor, mediates Wnt-PCP pathway during morphogenic movements of neural tube in the amphibian embryo. In this study, I attempted to generate a conditional knockout mouse of WGEF gene as a mammalian model of neural tube defects. So far, several conditional knockout allele on the WGEF locus was introduced by homologous recombination in the ES cells. Using this ES cell lines, chimera mice carrying WGEF conditional knockout cells have been generated.
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Report
(4 results)
Research Products
(23 results)
